Nikon TE2000-E inverted microscope

 Also available

Filter cubes

 Excitation filters

Emission filters

 Also available

1. Turn on the computer (#1)
2. Remove the cover from the microscope
3. Turn on the X-Cite exacte light (#2)
4. Turn on the microscope power bar (#3)
5. Use your UdeM credentials to log in to Windows
6. The first time you use the instrument, you need to import the microscope settings into the software. To do this follow the instructions First use protocol.
   
7. Start the software NIS

1. Save your data
2. If the oil objective was used, clean it with lens cleaner and lens paper (not Kimwipes)
3. Select the lowest magnification objective
4. Close the software NIS
   
5. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
6. Turn off the computer
7. Turn off the X-Cite exacte light (#2)
8. Turn off the microscope power bar (#3)
9. Cover the microscope

• Files can be saved temporarily (during acquisition) to local C: drive (desktop)
• At the end of each session, copy your data to your external drive and delete it from local C: drive
  
• You can store your files on the D: drive (Data Storage). If you do, please create one folder per laboratory using the principal investigator's last name. Inside, create a folder per user using the following nomenclature (First Name_Last Name).

When using the microscope for the first time, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

1. If open, close the software NIS and wait until it is completely closed (up to 30 seconds)
2. On your Desktop open the Softwares folder
3. Open NIS Settings Utility
4. Click on the Import tab
5. Click on Browse
6. Navigate to your Documents
7. Select the file NIS Settings for Nikon TE2000E.bin
8. Click Select
9. Select all items
10. Click Import
11. Click OK
12. Close NIS Settings Utility
13. You can now reopen NIS

• Perfect Focus avec LED de 780 nm et avec filtre dans le trajet lumineux LP 750 nm (inadéquat pour le Cy5.5)

• En lumière transmise, pour que le PFS fonctionne, il faut insérer 2 filtres IR dans le trajet lumineux: celui du PFS et celui du statif près de la lampe halogène

• Complete installation
• Complete cleaning

This can happen when the acquisition software is turned on before the camera has fully initialized.

• Turn off NIS-Elements
• Ensure the Initialize light at the back of the camera no longer blinks
• Start NIS-Elements

• Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
• The objectives are optimized to image through thin glass bottom multi-well plates
• You may also image specimen mounted between a slide and a 0.17mm thick coverslip
• For long timelapse, be aware of photo-toxicity