nVidia RTX A2000 with 12 GB GDDR6 dedicated memory
Monitor
Viewsonic VP3268a-4K native resolution 3840 x 2160
Softwares
ImageJ
FIJI
Zen Desk 3.11
Arivis Cloud Basic and Advanced
Intellesis Denoising and Object Classification
Direct and Advanced Processing, Extended Focus
2D and 3D Deconvolution
Image Analysis, Measurement and Colocalization
If not already done, turn on the computer (#1) and log in to Windows using your UdeM credentials
Start-up Zen
Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
In any case, your files should be removed from the C: drive.
Save your data
Close Zen
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
Direct Processing, Advanced Processing, Extended Focus, Deconvolution
Image Analysis, Measurement, Colocalization
Troubleshooting
This usually happens when the image files do not contain the correct metadata.
Make sure to save the files with all available metadata on the microscope used for the acquisition. This usually requires to save the files in the software proprietary format (.nd for NIS-Elements and Metamorph, .czi for Zen,...)
You can always add the correct calibration values after acquisition (pixel size in XY and Z, wavelength, Z-step)