1. If not already done, turn on the computer (#1) and log in to Windows using your UdeM credentials
2. Remove the dust cover from the microscope
3. Turn on the microscope power bar (#2) on the left of the computer

4. When using the instrument for the first time, it is necessary to import the microscope configuration before starting the software. See the First Use section below.

5. Start-up NIS-Elements

When using the instrument for the first time, it is necessary to import the microscope configuration into the software. This procedure is usually carried out during the training session. However, it can also be used to reset the software if it does not display correctly, for example.

1. If open, close NIS-Elements and wait until it is completely closed (up to 30 seconds)
2. On your Desktop open the Softwares folder
3. Open NIS Settings Utility
4. Click on the Import tab
5. Click on Browse
6. Navigate to your C:\Users\Public\Documents
7. Select the file Nikon_Ti2-Fura_NIS Settings.bin
8. Click Select
9. Select all items
10. Click Import
11. Click OK
12. Close the NIS Settings Utility
13. You can now reopen NIS-Elements

During this procedure, you will:

• Set the microscope in a safe configuration

• Load your sample

• Find and adjust the focus

Once completed, your sample will be ready for acquisition.

1. Save your data
2. Close NIS-Elements software
   
3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
4. Turn off the computer
5. If the oil objective was used, clean it with lens cleaner and lens paper (not Kimwipes)
6. Wait until the Spectra has cooled down and turn off the microscope power bar (#2)
7. Cover the microscope

• Files can be saved temporarily (during acquisition) to local C: drive (desktop)
• At the end of each session, copy your data to your external drive and delete it from local C: drive
  
• You can store your files on the D: drive (Data Storage). If you do, please create one folder per laboratory using the principal investigator's last name. Inside, create a folder per user using the following nomenclature (First Name_Last Name).

When using the microscope for the first time, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

1. If open, close NIS-Elements and wait until it is completely closed (up to 30 seconds)
2. On your Desktop open the Softwares folder
3. Open NIS Settings Utility
4. Click on the Import tab
5. Click on Browse
6. Navigate to your Desktop
7. Select the file Nikon Ti2-Fura settings for NIS.bin
8. Click Select
9. Select all items
10. Click Import
11. Click OK
12. Close the NIS Settings Utility
13. You can now reopen NIS-Elements

• Fix camera adapter
• Purchase 3mm adapter for Lapp
• Purchase multiband pass cube for Spectra
• Fix stage inserts
• Add Stage insert screws

• Complete installation
• Complete cleaning

This can happen when the acquisition software is turned on before the camera has fully initialized.

• Turn off NIS-Elements
• Ensure the Initialize light at the back of the camera no longer blinks
• Start NIS-Elements

• Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
• The objectives are optimized to image through thin glass bottom multi-well plates
• You may also image specimen mounted between a slide and a 0.17mm thick coverslip
• For long timelapse, be aware of photo-toxicity