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BD FACS Canto II
Roger Gaudry V-620
FACS Canto II has 3 solid-state laser sources. It can analyze up to 10 parameters and 8 fluorochromes simultaneously (4-2-2 configuration) .
- Applications
- Cytometry
Light Sources
405nm, 60mW
488nm, 20mW
633nm, 20mW
Resolution
FSC: 1 µm
SSC: 0.5 µm
Detectors
2 detectors for 405nm laser line
5 detectors for 488nm laser line
2 detectors for633nm laser line
405nm solid state 60mW trigon
PMT | Dichroic Mirror | Emission Filter | Compatible Fluorochromes |
---|---|---|---|
A | 502LP | 510/50 [485-535] | BV510, Pacific orange, Horizon V500, AmCyan, Autres BV* |
B | - | 450/50 [425-475] | BV421, Pacific Blue, Horizon V450, Alexa Fluor 405, eFluor450 |
C | - | - | - |
488nm solid state 20mW octagon
PMT | Dichroic Mirror | Emission Filter | Compatible Fluorochromes |
---|---|---|---|
A | 735LP | 780/60 [750-810] | PE-Cy7 |
B | 655LP | 450/50 [425-475] | PerCP-Cy5.5, PerCP, PerCP-eFluor 710 |
C | - | - | - |
D | 556LP | 585/42 [564-606] | PE, PI, Cy3 |
E | 502LP | 530/30 [515-545] | FITC, BB515, Alexa Fluor 488, eGFP, CFSE |
F | - | 488/10 [485-493] | SSC |
G | - | - | - |
H | - | - | - |
633nm HeNe 20mW trigon
PMT | Dichroic Mirror | Emission Filter | Compatible Fluorochromes |
---|---|---|---|
A | 735LP | 780/60 [750-810] | APC-Cy7, APC/Fire750, APC-AlexaFluor 750, APC-eFluor780 |
B | - | - | - |
C | - | 660/20 [650-670] | APC, Alexa Fluor 647, Alexa Fluor 633, eFluor660 |
- Instrument calibration is possible upon request. Please contact us.
- Turn on the computer
- Turn on the instrument
Log in Windows using your UdM credentials
- Start-up FacsDIVA
- Save your data
- Close FacsDIVA
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
Rappels Importants
- Take back your samples including ones in the instrument
- Leave the instrument and the working area clean
- Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
In any case, your files should be removed from the C: drive
Manuals
- Test the new workstation
- BD Fixe connection issue between FACSDIVA and Canto
To be completed
FACSDIVA does not start...
This can happen when the software can't connect to the instrument.
- Close FACSDIVA
- make sure the instrument is turned on
- Check the ethernet connection between the instrument and the computer
- Open FACSDIVA
Can I use this instrument remotely?
- No
- Aucune étiquette