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BD FACS Canto II

Roger Gaudry V-620

FACS Canto II has 3 solid-state laser sources. It can analyze up to 10 parameters and 8 fluorochromes simultaneously (4-2-2 configuration) .

  • Applications
    • Cytometry

  • Light Sources

    • 405nm, 60mW 

    • 488nm, 20mW

    • 633nm, 20mW

  • Resolution

    • FSC: 1 µm

    • SSC: 0.5 µm

  • Detectors

    • 2 detectors for 405nm laser line

    • 5 detectors for 488nm laser line

    • 2 detectors for633nm laser line

405nm solid state 60mW trigon

PMTDichroic MirrorEmission FilterCompatible Fluorochromes
A502LP510/50 [485-535]BV510, Pacific orange, Horizon V500, AmCyan, Autres BV*
B

-

450/50 [425-475]BV421, Pacific Blue, Horizon V450, Alexa Fluor 405, eFluor450
C---

488nm solid state 20mW octagon

PMTDichroic MirrorEmission FilterCompatible Fluorochromes
A735LP780/60 [750-810]PE-Cy7
B

655LP

450/50 [425-475]PerCP-Cy5.5, PerCP, PerCP-eFluor 710
C---
D556LP585/42 [564-606]PE, PI, Cy3
E502LP530/30 [515-545]FITC, BB515, Alexa Fluor 488, eGFP, CFSE
F-488/10  [485-493]SSC
G---
H---

633nm HeNe 20mW trigon

PMTDichroic MirrorEmission FilterCompatible Fluorochromes
A735LP780/60 [750-810]APC-Cy7, APC/Fire750, APC-AlexaFluor 750, APC-eFluor780
B

-

--
C-660/20 [650-670]APC, Alexa Fluor 647, Alexa Fluor 633, eFluor660
  • Instrument calibration is possible upon request. Please contact us.


  1. Turn on the computer
  2. Turn on the instrument

  3. Log in Windows using your UdM credentials

  4. Start-up FacsDIVA
  1. Save your data
  2. Close FacsDIVA
  3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive

Rappels Importants

  • Take back your samples including ones in the instrument
  • Leave the instrument and the working area clean
  • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from the local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

In any case, your files should be removed from the C: drive

  • Test the new workstation
  • BD Fixe connection issue between FACSDIVA and Canto

To be completed

FACSDIVA does not start...

This can happen when the software can't connect to the instrument.

  1. Close FACSDIVA
  2. make sure the instrument is turned on
  3. Check the ethernet connection between the instrument and the computer
  4. Open FACSDIVA

Can I use this instrument remotely?

  • No

Core Facilities CIB               Structural Biology    |    Cytometry    |    Microscopy    |    Histology