CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6fps at full frame
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User Guide
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User Guide
Startup
Turn on the computer
Turn on the microscope power bar
If fluorescence is required, turn on the mercury lamp power supply unit (3A) and press ignition(3B)
Warning
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
Log in Windows using your UdM credentials
Start-up NIS-Elements
Info
The first time you log in the computer, you need to import the microscope settings. To do this follow the instructions Nikon Eclipse E600-en
Shutdown
Save your data
Close NIS-Elements
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
Get your samples from the microscope
If used, clean the oil objective with lens cleaner and lens paper
If fluorescence was used, turn off the mercury lamp power supply unit (3A)
Turn off the microscope power bar (2)
Wait until the lamps are cool and cover the microscope
Important Reminders
Take back your samples including ones in the microscope
Leave the microscope and the working area clean
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
Storage Management
Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
In any case, your files should be removed from the C: drive.
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Setting-up NIS-Elements
Setting-up NIS-Elements
Setting-up NIS-Elements
This is required the first time you are using the instrument. You will usually do it during the training session. It can be performed if something is not working properly right or if you want to refresh the software interface.
Note
This process will delete all experiment protocols and restore the parameters for the microscope
Close NIS-Elements
Wait until the complete closure of NIS-Elements
Open the folder Desktop\Logiciels
Open the software NIS Settings Utility
Click on the Importtab
Click on Browse
Navigate to your desktop
Select the file Nikon-E600 Settings.bin
Click Select
Select all items
Click Import
Click OK
Close the NIS Settings
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Lightpath
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Lightpath
Follow the schematics below for Transmitted light (Bright field, Phase Contrast, Polarized light) and transmitted light (fluorescence).