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| Nikon Eclipse E-600 upright microscope Roger Gaudry N-620 Applications Brightfield Phase Contrast Polarized light Fluorescence
Light sources Objectives 10x/0.3 Air Ph1 WD 16 40x/0.75 Air Ph2 WD 0.75 100x/1.3 Oil Ph3 WD 0.2
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| title | Objectives complete specifications |
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| Position | Name | Brand | ID | Magnification | Numerical Aperture | Immersion | Type | Working distance (mm) | Transmittance
(% [nm]) | Technique | Cover glass thickness (mm) |
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| 1 | 10x/0.3 Air | Nikon | 10x/0.3 Air Plan Fluor Ph1 DLL | 10x | 0.3 | Air | Plan Fluor | 16 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 | | 2 | 40x/0.75 Air | Nikon | 40x/0.75 Air Plan Fluor Ph2 DLL | 40x | 0.75 | Air | Plan Fluor | 0.72 | >80% [400-750] | BF, Pol, PhC, Fluo | 0.17 | | 3 | | Nikon | 100x/1.3 Oil Plan Fluor Ph3 DLL | 100x | 1.3 | | Plan Fluor | 0.2 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 | | 4 | Empty |
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Filter cubes DAPI/Hoechst/AMCA FITC/EGFP TRITC/Rhodamine TexasRed
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| title | Filters complete specifications |
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| id | User Guide |
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| title | User Guide |
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| - Turn on the computer
- Turn on the microscope power bar
If fluorescence is required, turn on the mercury lamp power supply unit (3A) and press ignition (3B)
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Mercury lamp must be on for at least 30 min before being turned off and vice-versa |
- Log in Windows using your UdM credentials
- Start-up NIS-Elements
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The first time you log in the computer, you need to import the microscope settings. To do this follow the instructions Setting-up NIS-Elements |
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| - Save your data
- Close NIS-Elements
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
- Get your samples from the microscope
- If used, clean the oil objective with lens cleaner and lens paper
- If fluorescence was used, turn off the mercury lamp power supply unit (3A)
- Turn off the microscope power bar (2)
- Wait until the lamps are cool and cover the microscope
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| - Take back your samples including ones in the microscope
- Leave the microscope and the working area clean
- Mercury lamp must be on for at least 30 min before being turned off and vice-versa
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| - Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
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In any case, your files should be removed from the C: drive. |
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| title | Setting-up NIS-Elements |
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| Setting-up NIS-Elements |
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| Setting-up NIS-Elements |
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This process is required the first time you are using the instrument. You will usually do it during the training session. It can also be performed if something is not working properly right or if you want to refresh the software interface. | Note |
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This process will delete all experiment protocols and restore the parameters for the microscope. |
- Close NIS-Elements
- Wait until the complete closure of NIS-Elements
- Open the folder Desktop\Logiciels
- Open the software NIS Settings Utility
- Click on the Import tab
- Click on Browse
- Navigate to your desktop
- Select the file Nikon-E600 Settings.bin
- Click Select
- Select all items
- Click Import
- Click OK
- Close the NIS Settings
- Open NIS-Elements
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| id | Lightpath |
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| title | Light path |
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| Follow the schematics below for Transmitted light (Bright field, Phase Contrast, Polarized light) and transmitted light (fluorescence). Light path schematics.pdf |
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| - Adjust focus drive jitter
- Add a holder for polarized light analyzer
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| - Nikon Preventive Maintenance
- Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
- 100x slightly damaged but not the lens
- Fluorescence cubes damaged: FITC TexasRed
- Focus drive has a jitter
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| - Replacement mercury lamp bulb HBO 1003W/2
- 256 GB SSD added in workstation for OS
- Windows 10 Installation
- BIOS updated to v2.47
- Camera Firmware updated to v2.11
- NIS-Elements Basic Research v4.6 64-bits installed
- Creation NIS Elements Settings
- FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK
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| id | Technical Datasheet |
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| title | Technical Datasheet |
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| Stand - Nikon Eclipse E600 upright Serial 725540
Light sources - Transmitted light Halogen 12V 100W Serial 01875599
- Reflected light
- Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
- Bulb housing model LH M100C-1 Serial 039326
Condenser - Condenser Universal C-CU Serial 081901
- Lens Dry NA 0.9
- Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty
Objectives - 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
- 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
- 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
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- Empty
- Empty
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| title | Detailed specifications |
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| Position | Name | Brand | ID | Magnification | Numerical Aperture | Immersion | Type | Working distance (mm) | Transmittance
(% [nm]) | Technique | Cover glass thickness (mm) |
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| 1 | 10x/0.3 Air | Nikon | 10x/0.3 Air Plan Fluor Ph1 DLL | 10x | 0.3 | Air | Plan Fluor | 16 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 | | 2 | 40x/0.75 Air | Nikon | 40x/0.75 Air Plan Fluor Ph2 DLL | 40x | 0.75 | Air | Plan Fluor | 0.72 | >80% [400-750] | BF, Pol, PhC, Fluo | 0.17 | | 3 | | Nikon | 100x/1.3 Oil Plan Fluor Ph3 DLL | 100x | 1.3 | | Plan Fluor | 0.2 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 | | 4 | Empty |
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Turret DAPI/Hoechst/AMCA FITC/EGFP TRITC/Rhodamine TexasRed
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| title | Detailed specifications |
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Detector - Color Camera Nikon DS-Ri2 Serial 701234
Workstation - HP Z440 Workstation
- Intel Xeon E5-1630 v3 @ 3.7GHz
- RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
- OS 256GB SSD 550 MBs
- 2TB HD Data Storage 150 MBs
- Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
- Monitor HP Z24i display 24' 1920x1200
Consumables |
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| id | FAQ |
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| title | Troubleshooting & FAQ |
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| title | Troubleshooting |
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| NIS-Elements shows an error: Camera Driver... This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on. - Turn off NIS-Elements
- Turn on the camera (the microscope power bar and the camera switch on the top of the camera)
- Check the USB conexion between the camera and the computer
- Turn on NIS-Elements
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| Can I use this microscope to look at cell in a dish? - No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.
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