Comparaison des versions

Ancienne version 1

changes.mady.by.user Nicolas Stifani

Sauvegardée le

comparé à

Nouvelle version 2

changes.mady.by.user Nicolas Stifani

Sauvegardée le

Légende

  • Ces lignes ont été ajoutées. Ce mot a été ajouté.
  • Ces lignes ont été supprimées. Ce mot a été supprimé.
  • La mise en forme a été modifiée.

Inclure page
_head-en
_head-en

Button Hyperlink
iconbuild
titleFrançais
typeprimary
urlhttps://wiki.umontreal.ca/display/Microscopie/Nikon+Eclipse+E600




Tabs Container
idInstrument Tabs
titleNikon Eclipse E600
directionhorizontal

Tabs Page
idDescription
titleDescription

Nikon

Eclipse E-600 upright

Ti2-E fully motorized inverted microscope

Roger Gaudry NR-620619

  • Applications

    • Brightfield

    • Phase Contrast

    • Polarized light

    • Fluorescence

  • Light sources

    • Halogen LED lamp for transmitted light

    • Mercury lamp (350~600nm) for fluorescence 

    • Lumencor SpectraX laser fluorescence 

Développer
titleLumencor SpectraX complete specifications


Filter nameExcitation FilterTypeWorking distance (mm)Transmittance
(% [nm])
R

640/30-25

Plan Fluor2.1>75% [400-800]
G

470/24-25


Excitation FilterDichroic mirrorEmission Filter
B

440/20-25

350/50x [325-375]400LP460/50m [435-485]
NT

510/25-25

Plan Apo Lambda0.13
V

395/25-25




GY550\15-25Plan Apo Lambda

TBD


Storage

GY

575\25-25



  • Objectives

    • 20x/0.5 Air Ph1
    • 60x/1.4 Oil DIC WD 0.13
    • 100x/1.45 Oil Ph3 WD 0.13
    • 100x/1.45 Oil DIC WD 0.13
    • Empty
    • 20x\0.75 Air DIC 

    Objectives

    • 10x/0.3 Air Ph1 WD 16

    • 40x/0.75 Air Ph2 WD 0.75

    • 100x/1.3 Oil Ph3 WD 0.2
Développer
titleObjectives complete specifications


PositionNameBrandIDMagnificationNumerical ApertureImmersionTypeWorking distance (mm)Transmittance
(% [nm])		TechniqueCover glass thickness (mm)
110x20x/0.3 5 Air Ph1Nikon10x

20x/0.

3 Air

5 Plan Fluor

Ph1 DLL

WD 2.1 Ph1? Air

20x10x0.35AirPlan Fluor162.1>75% [400-800]BF, Pol, PhC, Fluo0.17
2

40x60x/0.75 Air1.4 Oil DIC

Nikon40x60x/01.75 Air Plan Fluor Ph2 DLL40x

0.75

Air

Plan Fluor4 Plan Apo Lambda DIC N260x

1.4


Remarque
Oil


Plan Apo Lambda0.130.72>80% [400-750]BF, Pol, PhCDIC, Fluo0.17
3

100x/1.345 Oil Ph3

Nikon100x/1.3 Oil 45 Plan Fluor Apo Lambda Ph3 DLL

100x

1.345


Remarque
Oil


Plan FluorPPlan Apo Lambda0.213>75% [400-800]BF, Pol, PhCDIC, Fluo0.17
4Empty100x/1.45 Oil DICNikon100x/1.45 Plan Apo Lambda DIC N2

100x

1.45


Remarque
Oil


Plan Apo Lambda0.13
BF, Pol, DIC, Fluo
5Empty









6Empty20x/0.75 Air DICNikon20x/0.75 Plan Apo Lambda DIC N220x0.75AirPlan Apo Lambda

TBD


BF, Pol, DIC, Fluo


  • Filter cubes

    • DAPI/Hoechst/AMCA

    • FITC/EGFP

    • TRITC/Rhodamine

    • TexasRed

Développer
titleFilters complete specifications


PositionCube nameBrandIDExcitation FilterDichroic mirrorEmission Filter
1DAPI/Hoechst/AMCAChromaCube set 31000v2350/50x [325-375]400LP460/50m [435-485]
2FITC/EGFPChromaCube set 41001

480/40x [420-500]

505LP

535/50m [510-560]
3TRITC/RhodamineChroma

Cube set 41002c

545/30x [530-560]570LP620/60m [590-650]
4Texas RedChroma

Cube set 41004

560/55x [532-588]

595LP645/75m [607-682]
5Empty




6Empty





  • Detector

    • Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6fps at full frame


Tabs Page
idUser Guide
titleUser Guide


UI Expand
expandedtrue
titleStartup
  1. Turn on the computer
  2. Turn on the microscope power bar

  3. If fluorescence is required, turn on the mercury lamp power supply unit (3A) and press ignition (3B)


    Avertissement

    Mercury lamp must be on for at least 30 min before being turned off and vice-versa


  4. Log in Windows using your UdM credentials
  5. Start-up NIS-Elements
Info

The first time you log in the computer, you need to import the microscope settings. To do this follow the instructions Setting-up NIS-Elements



UI Expand
titleShutdown
  1. Save your data
  2. Close NIS-Elements
  3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  4. Get your samples from the microscope
  5. If used, clean the oil objective with lens cleaner and lens paper
  6. If fluorescence was used, turn off the mercury lamp power supply unit (3A)
  7. Turn off the microscope power bar (2)
  8. Wait until the lamps are cool and cover the microscope
Remarque
titleImportant Reminders
  • Take back your samples including ones in the microscope
  • Leave the microscope and the working area clean
  • Mercury lamp must be on for at least 30 min before being turned off and vice-versa



UI Expand
titleStorage Management
  • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from the local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
Remarque

In any case, your files should be removed from the C: drive.



UI Expand
titleSetting-up NIS-Elements

Ancre
Setting-up NIS-Elements
Setting-up NIS-Elements

This process is required the first time you are using the instrument. You will usually do it during the training session. It can also be performed if something is not working properly right or if you want to refresh the software interface.

Remarque

This process will delete all experiment protocols and restore the parameters for the microscope.

  1. Close NIS-Elements
  2. Wait until the complete closure of NIS-Elements
  3. Open the folder Desktop\Logiciels
  4. Open the software NIS Settings Utility
  5. Click on the Import tab
  6. Click on Browse
  7. Navigate to your desktop
  8. Select the file Nikon-E600 Settings.bin
  9. Click Select
  10. Select all items
  11. Click Import
  12. Click OK
  13. Close the NIS Settings
  14. Open NIS-Elements



Tabs Page
idLightpath
titleLight path

Light path

Download the schematics to follow Transmitted light (Bright field, Phase Contrast, Polarized light) and Reflected light (fluorescence) paths.

Light path schematics.pdf


Tabs Page
idManuals
titleManuals

Manuals


Tabs Page
idLog
titleLog


UI Expand
expandedtrue
titleTo do
  • Adjust focus drive jitter
  • Add a holder for polarized light analyzer


UI Expand
title2018-04-04
  • Nikon Preventive Maintenance
  • Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
  • 100x slightly damaged but not the lens
  • Fluorescence cubes damaged: FITC TexasRed
  • Focus drive has a jitter


UI Expand
title2021-07-20
  • Replacement mercury lamp bulb HBO 1003W/2
  • 256 GB SSD added in workstation for OS
  • Windows 10 Installation
  • BIOS updated to v2.47
  • Camera Firmware updated to v2.11
  • NIS-Elements Basic Research v4.6 64-bits installed
  • Creation NIS Elements Settings
  • FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK



Tabs Page
idTechnical Datasheet
titleTechnical Datasheet

Stand

  • Nikon Eclipse E600 upright Serial 725540

Light sources

  • Transmitted light Halogen 12V 100W Serial 01875599
  • Reflected light
    • Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
    • Bulb housing model LH M100C-1 Serial 039326

Condenser

  • Condenser Universal C-CU Serial 081901
  • Lens Dry NA 0.9
  • Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty

Objectives

  • 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
  • 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
  • 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
  • Empty
  • Empty
  • Empty
Développer
titleObjectives complete specifications


PositionNameBrandIDMagnificationNumerical ApertureImmersionTypeWorking distance (mm)Transmittance
(% [nm])		TechniqueCover glass thickness (mm)
110x/0.3 AirNikon10x/0.3 Air Plan Fluor Ph1 DLL10x0.3AirPlan Fluor16>75% [400-800]BF, Pol, PhC, Fluo0.17
2

40x/0.75 Air

Nikon40x/0.75 Air Plan Fluor Ph2 DLL40x

0.75

Air

Plan Fluor0.72>80% [400-750]BF, Pol, PhC, Fluo0.17
3

100x/1.3 Oil

Nikon100x/1.3 Oil Plan Fluor Ph3 DLL

100x

1.3


Remarque
Oil


Plan Fluor0.2>75% [400-800]BF, Pol, PhC, Fluo0.17
4Empty









5Empty









6Empty










Filters

  • DAPI/Hoechst/AMCA

  • FITC/EGFP

  • TRITC/Rhodamine

  • TexasRed

Développer
titleFilters complete specifications


PositionCube nameBrandIDExcitation FilterDichroic mirrorEmission Filter
1DAPI/Hoechst/AMCAChromaCube set 31000v2350/50x [325-375]400LP460/50m [435-485]
2FITC/EGFPChromaCube set 41001

480/40x [420-500]

505LP

535/50m [510-560]
3TRITC/RhodamineChroma

Cube set 41002c

545/30x [530-560]570LP620/60m [590-650]
4Texas RedChroma

Cube set 41004

560/55x [532-588]

595LP645/75m [607-682]
5Empty




6Empty





Detector

  • Color Camera Nikon DS-Ri2 Serial 701234

Workstation

  • HP Z440 Workstation
  • Intel Xeon E5-1630 v3 @ 3.7GHz
  • RAM 32 GB DDR4 2133 MHz  (4 x 8 GB)
  • OS 256GB SSD 550 MBs
  • 2TB HD Data Storage 150 MBs
  • Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
  • Monitor HP Z24i display 24' 1920x1200

Consumables


Tabs Page
idFAQ
titleTroubleshooting & FAQ


UI Expand
expandedtrue
titleTroubleshooting

NIS-Elements shows an error: Camera Driver...

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

  • Turn off NIS-Elements
  • Turn on the camera (the microscope power bar and the camera switch on the top of the camera)
  • Check the USB conexion between the camera and the computer
  • Turn on NIS-Elements


UI Expand
titleFAQ

Can I use this microscope to look at cell in a dish?

  • No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.





Tabs Container
idDemo Image
directionhorizontal


Tabs Page
idDemo Images
titleDemo Images

Image Removed

Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)

Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)

Image Added