Historique de la page

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• Link Page
  name SAXS Instrumentation linkText Instrumentation type page

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  • Overview of the instrumentation - to be familiar with the equipment in the laboratory
  • Software - software used by the instrument
  • Maintenance - basic maintenance procedures

• Link Page
  name SAXS experiment linkText SAXS experiment type page

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  • Running a SAXS experiment - first steps into data acquisition
  • SEC-SAXS setup - data acquisition in SEC-SAXS mode

• Link Page
  name SAXS data processing linkText Data processing

  Data processing:
  • Basic processing of SAXS data - initial data analysis and sample quality assessment
  • Advanced processing of SAXS data - calculation of the pairwise distribution function
  • Dealing with SEC-SAXS data - analyzing your SEC-SAXS data

• Link Page
  name Ab initio modeling linkText Ab initio modeling

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  • Modeling with DAMMIF - dummy atom modeling from SAXS data using DAMMIF
  • Modeling with DENSS - electron density modeling from SAXS data using DENSS

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Alternatively, the Structural Biology Platform has computers available where recent versions are installed. Access to the computer room is controlled by access codes. Contact Normand Cyr in order to obtain a code.

Scientific literature

• Jeffries CM, et al (2016). Preparing monodisperse macromolecular samples for successful biological small-Angle X-ray and neutron-scattering experiments. Nature Protocols.
• Putnam CD, et al (2007). X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Quarterly Reviews of Biophysics
• Skou S, et al (2014). Synchrotron-based small-angle X-ray scattering of proteins in solution. Nature Protocols.
• Trewhella J, et al (2017). 2017 publication guidelines for structural modeling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallographica Section D.