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Sample requirement
As stated previously, SAXS is a contrast method. Whereas in batch mode we need to record data for both the buffer alone and the sample in buffer, in SEC-SAXS, we continuously record SAXS images as the chromatography takes place. These images will include data from both the buffer (the actual mobile phase from the chromatography - before the sample elution for example) and from the samples in buffer (during elution).
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