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In the present case, d should be at least 15 mm, ideally 20 mm. This corresponds to about 500 550 µl in a 5 mm tube. Do not hesitate to confirm with the NMR manager that your tube is properly positioned.
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Then, in TopSpin, on the control computer, enter the command ej to activate the air lift and if it is the case, eject the sample already present in the magnet. Climb up the stairs and replace the previous sample in the bore (red touretturret) with yours. Make sure the air lift is activate activated so that your sample does not fall in the bore. The pneumatic system should be powerful enough to maintain your sample on top.
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The temperature set on the computer is slightly different than from the actual temperature in the magnet.
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Modern NMR magnets do not drift much beyond 4 to 5 Hz per hour. Yet, this would be sufficient to lose spectral resolution (eg J-coupling) over a 1-hour experiment. As a reference, below are the current (as of February 2020) magnet drifts for our NMR spectrometers.
| Magnet | Drift, Hz/h |
|---|---|
| 500 MHz | 2.4 |
| 600 MHz | 0.2 |
| 700 MHz | 0.2 |
Tune and match
Shim
Data acquisition
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