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Bruker has put together a calibration routine which (1) automates the determination of the optimal O1 and P1, and (2) records a 1D proton spectrum of the sample (using the zgesgp pulse program). The AU program to launch is called calibo1p1. The program will run

• o1calib
• pulsecal
• run a 1-minute excitation sculpting 1D proton experiment (zgesgp)

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Then the values of O1 and P1 can be transferred retrieved by looking at the recording parameters in the resulting experiment (eda). Write them down and transfer them to the following experiments. You need to make sure you execute getprosol in the other experiments to calculate all the pulses based on P1.

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For example: getprosol 1H 8.60 12.614W614W

Another advantage of the calibo1p1 program is that you can assess the quality of your sample by looking at the 1D proton spectrum it produces (see above). Well dispersed peaks, particularly in the amide protons region (6-10 ppm) is indicative of a diverse chemical environment around these hydrogen atoms, typically correlated with structural elements.

Data acquisition

Processing

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