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These are cells exposed to 1 second of 561nm 70mW of 550nm light every 5 minutes. The total movie is about 3h. The cells retract, detach and eventually die.
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This is another example of cells exposed to 1 second of 59mW of 405nm 395nm and 1s of 561nm 70mW of 550nm light every 5 minutes. The total duration is about 8h.
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If you step back then you can see that the damaged are actually limited to the exposed area. Note that the recorded area is smaller than the exposed area.
What are the important factors when talking about photo-toxicity?
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The wavelength: The energy carried by laser light is dependant dependent of the wavelength. Shorter wavelength have more energy and are more deleterious
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With a power meter you can also evaluate the amount of energy that your cells are confortable comfortable with.
To do this measure the power at the objective using your regular imaging settings.
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