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It is always good to acquire a larger field of view of the recorded area to make sure no photo-toxicity has occurred.
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With a power meter you can also evaluate the amount of energy that your cells are comfortable with.
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Here is another discrete example.
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Cells were imaged for 100ms with 70mW of 550nm light every 5 minutes. Cells are dividing faster than the effect of photo-toxicity that is occurring. Again here the best way to control is to take an overview image at the end of the acquisition and compare exposed cells to non-exposed cells.
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