HBO Mercury lamp emission spectra (Source)
Comparison HBO Mercury vs XCite Metal Halide Lamps (Source)
OSRAM HBO 100W/2 Mercury lamp manual (pdf)

1. Turn on the computer (#1)
2. Turn on the microscope power bar (#2)

3. If fluorescence is required, turn on the mercury lamp power supply unit (#3A) and press ignition (#3B)
   

   Avertissement
   Mercury lamp must be on for at least 30 min before being turned off and vice-versa

   
   

4. If transmitted light is required, turn on the halogen lamp on the right side of the microscope (#4)

5. Log in Windows using your UdM credentials

6. Start-up NIS-Elements

1. Save your data
2. Close NIS-Elements
3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
4. Turn off the computer
5. If used, clean the oil objective with lens cleaner and lens paper
6. If fluorescence was used, turn off the mercury lamp power supply unit (#3A)
7. If transmitted light was used, turn off the halogen lamp on the right side of the microscope (#4)
8. Turn off the microscope power bar (#2)
9. Wait until the lamps have cooled and cover the microscope

• Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
• At the end of each session, copy your data to your external drive and delete it from the local C: drive
• You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

This process is required the first time you are using the instrument. You will usually do it during the training session. It can also be performed if something is not working properly or if you want to reset the software interface.

1. Close If open, close NIS-Elements
2. Wait until the complete closure of NIS-Elements
3. Open the folder Desktop\Logiciels
4.  and wait until it is completely closed (up to 30 seconds)
5. On your Desktop open the Softwares folder
6. Open Open the software NIS Settings Utility
7. Click on the Import tab
8. Click on Browse
9. Navigate to your desktop Desktop
10. Select the file Nikon-E600 Settings.bin
11. Click Select
12. Select all items
13. Click Import
14. Click OK
15. Close the NIS Settings
16. Open You can now reopen NIS-Elements

• Adjust focus drive jitter
• Add a holder for polarized light analyzer

• Nikon Preventive Maintenance
• Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
• 100x slightly damaged but not the lens
• Fluorescence cubes damaged: FITC TexasRed
• Focus drive has a jitter

• Replacement mercury lamp bulb HBO 1003W/2
• 256 GB SSD added in workstation for OS
• Windows 10 Installation
• BIOS updated to v2.47
• Camera Firmware updated to v2.11
• NIS-Elements Basic Research v4.6 64-bits installed
• Creation NIS Elements Settings
• FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

• Turn off NIS-Elements
• Turn on the camera (both the microscope power bar and the camera need to be ON
  (the camera is usually ON, the switch on the top of the camera)
• Check the camera is correctly connected to the computer via a USB cable
• Turn on NIS-Elements

No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.