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Button Hyperlink
iconbuild
titleFrançais
typeprimary
urlhttps://wiki.umontreal.ca/display/Microscopie/GE+InCell+Analyzer+6000


Tabs Container
idInstrument Tabs
titleInCell Analyzer
directionhorizontal


Tabs Page
idDescription
titleDescription

GE InCell Analyzer 6000 High content microscope

JA Bombardier Building, Room 3129
Instrument awarded to Dr. Steve Michnick by the Canadian Foundation for Innovation (CFI)

Advanced Microscope Tier 2 usage price

  • Applications
    • Transmitted light
    • Pseudo phase contrast and DIC
    • Fluorescence
    • High-throughput imaging
    • Long-term imaging

  • Light sources

    • LED for transmitted light

    • Toptica iChrome MLE for fluorescence

Développer
titleToptica iChrome complete caracteristicsspecifications


SourcePolychroicExcitation wavelength (nm)Compatible fluorophoresNominal Power
(mW)
405nm390/40[370-410]DAPI, Hoechst50
488nm

482/18

[473-491]FITC, GFP, YFP

25

561nm

564/9

[559-569]

Cy3, DsRed, TxRed

30
642nm640/14[632-647]

Cy5, Cy5.5

50


  • Objectives

    1. 10x/0.45 Air WD 4.0
    2. 20x/0.75 Air WD 1.0
    3. 40x/0.6 Air WD 2.7-3.7
    4. 60x/0.95 Air WD 0.15


Développer
titleCaractéristiques complètes des objectifsObjective specifications


PositionNameBrandFull nameIdentifierWorkinf distance (mm)Transmittance
(% [nm])		TechnicCoverslip thickness (mm)
110x/0.45 AirNikon

10x/0.45 Air
Plan Apo
M25x0.75

MRD001014.0>80% [TBD-TBD]BF, p-PhC, p-DIC, Fluo0.17
2

20x/0.75 Air

Nikon20x/0.75 Air
Plan Apo
DIC N2
M25x0.75		MRD002011.0>80% [TBD-TBD]BF, p-PhC, p-DIC, Fluo0.17
3

40x/0.6 Air

Nikon40x/0.6 Air
Plan Fluor ELWD
M25x0.75		MRH084302.7-3.7>80% [TBD-TBD]BF, p-PhC, p-DIC, Fluo0.17
460x/0.95 Air Nikon60x/0.95 Air 
Plan Apo
M25x0.75		MRD006000.15>80% [TBD-TBD]BF, p-PhC, p-DIC, Fluo0.17

BF: Bright-field
p-PhC: Pseudo-phase contrast
p-DIC: Pseudo-Differetial interference contrast

  • Cubes de filtres

    1. DAPI

    2. GFP

    3. Cy3

    4. Cy5

    5. Cy5.5
Développer
titleFilter specifications


PositionNomMarqueIdentifiant

Miroir polychroïque

(Transmission)

Filtre d'émissionBande passante effective
1DAPITBDTBDBP 420/20
[430-462]		455/50
[430-480]		[430-480]
2GFPTBDTBD

BP 446/32; 524/42; 600/36; 732/137

525/20
[515-535]		[515-535]
3Cy3TBD

TBD

BP 446/32; 524/42; 600/36; 732/137605/52
[579-631]		[582-618]
4

Cy5

TBD

TBD

BP 446/32; 524/42; 600/36; 732/137707/72
[671-743]		[671-743]
5

Cy5.5

TBDTBDBP 446/32; 524/42; 600/36; 732/137720/60
[690-750]		[690-750]




Tabs Page
idLog
titleLog


UI Expand
titleTo do


UI Expand
title2021-09-27
  • Added to wiki



Tabs Page
idFAQ
titleTroubleshooting & FAQ


UI Expand
titleTroubleshooting



UI Expand
titleFAQ

Can I use this microscope to look at cell in a dish?

  • No. This a microscope designed to acquire images of specimen in multi-well plates
  • You may also image specimen mounted between a slide and a 0.17mm thick coverslip





Tabs Container
idDemo Image
titleInCell Analyzer
directionhorizontal


Tabs Page
idDemo Image
titleDemo Image




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