Comparaison des versions
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7. Complete filter specifications
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Position
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Nom
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Marque
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Identifiant
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Filtre d'excitation
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Miroir dichroïque
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Filtre d'émission
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Commentaire
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1
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Empty
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2
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BS_455
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Zeiss
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455LP
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To be used with FRAP 405
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3
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LSM Duo T20/R80 UV
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Zeiss
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4
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Brightfield Ref Light
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Zeiss
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5
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Set 76 CFP GFP DsRed
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Zeiss
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489076-0000-000
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390-422 484-501 549-573
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427 503 578
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448-472 512-538 585-631
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6
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Set 77 GFP/RFP/Alexa633
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Zeiss
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489077-0000-000
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469-497 552-577 629-650
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506 582 659
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The following diagrams allow you to follow the light path in transmitted light (bright field, DIC) and in reflected light (fluorescence).
To do
Check Loss of 488nm laser power
2024-04-26
(FIXED) Problem with the FRAP module:
At first could not modulate the laser intensity (yet could not find the spot on the field), rebooted and then the FRAP fiber does not carry any light (even 488)
==> Laser manipulation module was too far in x and y (noticeable as a tilt and a gap between both parts of the module).
2024-03-04
Corrected Trigger Module configuration for the BackCam
- reassigned each camera with its own trigger module in MTB config
2024-02-29
- DualCam calibration parameters set, using beads
- Realigned Lasers:
- Laser output measured in spinning disk mode at the sample with 10x objective, 1x Extended tubelens, 100% laser power and ALL lasers ON:
- 405nm: 0.70 mW before => 0.66 mW after
- with laser 488 OFF: 0.81 mW before => 0.83 mW after
- 488nm: 3.75 mW before => 4.17 mW after
- with laser 405 OFF: 3.95 mW before => 4.33 mW after
- 561nm: 2.1 mW before => 2.37 mW after
- 639nm: 0.9 mW before => 1.1 mW after
- 405nm: 0.70 mW before => 0.66 mW after
- Laser output measured in spinning disk mode at the sample with 10x objective, 1x Extended tubelens, 100% laser power and ALL lasers ON:
- NOTE: AOTF2 for FRAP
AOTF2 calibration
For FRAP, the illumination is strongest with the AOTF2 at 60% and not 100%
· Error message when switching between FRAP & SD illumination
AOTF2 Error message
FRAP/SD illumination switching triggers an error message " 'MTBAOTF2LaserLine6' is not supported by the current hardware"
2021-11-20
- Zen 2.6 updated hotfix 12
- Microsoft Windows update
- Laser output measured in spinning disk mode at the sample with 10x objective, 1x Extended tubelens, 100% laser power and ALL lasers ON:
- 405nm: 1.08 mW StdDev 0.01mW
o 488nm: 3.3 mW StdDev <0.01mW (3.7 mW if laser 405 is OFF)
405nm and 488nm Interactions
When both 405nm and 488nm laser are ON simultaneously, the output power is decreased by 11%.
- 561nm: 2.6 mW StdDev <0.01mW
- 639nm: 1.37 mW StDev 0.02 mW
· Control for vibrations during construction work on the 4th floor
2021-11-03
- Error when the definite focus is used (unexpected error the definite focus did not respond)
- To solve it:
- Turn off the Zen Software
- Turn off the microscope power bar (#3)
- Turn off the definite focus by pressing and holding the definite focus button
- Turn on the definite focus by pressing once the definite focus button
- Wait until the definite focus display shows "Detecting stand"
- Turn on the microscope power bar (#3)
- Confirm that the definite focus is showing in the microscope tactile display
- Open Zen software and run an experiment using the definite focus to confirm it is fully functional
2021-09-27
- Added to wiki
2020-07-07
- AOTF1 changed