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id | Description |
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title | Description |
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| Nikon Ti2-E fully motorized inverted microscopeRoger Gaudry Building, Room R-619 Instrument awarded to Dr Paradis-Bleau the Canadian Foundation for Innovation (CFI) Advanced Microscope Tier 1 usage price
Applications - BrightfieldTransmitted light, Bright-field
- Phase contrast
Polarized light, DIC Fluorescence - Live imaging
- Long timelapse Time-lapse imaging
Light sources
Développer |
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title | Lumencor SpectraX complete specifications |
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Filter nameSource | Filter ID | Filter type | Excitation wavelengths (nm) | Typical Compatible fluorophoreluorophores | Nominal power (mW) |
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Violet | 395/25 | R | 640/30 | Bandpass | [ | 625382- | 655407] | Cy5 | G | DAPI, Hoechst | 295 | Blue | | 470/24 | Bandpass | [ | 458430- | 482450] | CFP | FITC, GFP256 | BCyan | 440470/ | 2024 | Bandpass | [ | 430458- | 450482] | CFP | FITC, GFP | 196 | Teal | NT | 510/25 | Bandpass | [497-522] | YFP | 62 | Green/Yellow | 550/15 | V | 395/25 | Bandpass | GY | 550\15 | Bandpass | [ 382-407] | DAPI, Hoechst | [542542-557] | TRITC, Cy3 | 260 | GY Green/Yellow (Storage) | 575 | \/25 | Bandpass | [562-587] | mCherry | 310 | Red | 640/30 | Bandpass | [625-655] | Cy5 | 231 |
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Objectives - 20x/0.5 Air Ph1 WD 2.1
- 60x/1.4 Oil DIC WD 0.13
- 100x/1.45 Oil Ph3 WD 0.13
- 100x/1.45 Oil DIC WD 0.13
- Empty
20x\- 4x/0.
75 Air DIC - 2 Air WD 20
- 20x/0.75 Air DIC WD 1.0
Développer |
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title | Objectives complete specifications |
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Position | Name | Brand | ID | Magnification | Numerical Aperture | Immersion | Type | Full name | Identifier | Working distance (mm) | Transmittance (% [nm]) | TechniqueTechniques | Cover glass thickness (mm) |
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1 | 20x/0.5 Air Ph1 | Nikon | 20x/0.5 Air Plan Fluor | WD 2.1 Ph1 | ? Air | 20x | 0.5 | Air | Plan Fluor | MRH10201 | 2.1 | >75% >80% [400- | 800750] | BF, Pol, PhC, Fluo | 0.17 | 2 | 60x/1.4 Oil DIC | Nikon | 60x/1.4 Oil Plan Apo Lambda DIC N260x | MRD01605 | 10.4 | Plan Apo Lambda | 0.13 | >75% [400-80013 | >80% [475-725] | BF, Pol, DIC, Fluo | 0.17 | 3 | | Nikon | 100x/1.45 Oil Plan Apo Lambda Ph3 | 100x | 1.45 | MRD31905 | PPlan Apo Lambda | 0.13 | >75% >80% [ | 400475- | 800750] | BF, Pol, | DIC PhC, Fluo | 0.17 | 4 | 100x/1.45 Oil DIC | Nikon | 100x/1.45 Oil Plan Apo Lambda DIC N2 | 100x | 1.45 | MRD01905 | Plan Apo Lambda | 0.13 | >80% [ | 400475-750] | BF, Pol, DIC, Fluo | 0.17 | 5 | Empty | 6 | 20x4x/0.2 Air | Nikon | 4x/0.2 Air Plan Apo Lambda | MRD00045 | 20 | >80% [400-1000] | BF, Fluo | 0.17 | 6 | 20x/0.75 Air DIC | Nikon | 20x/0.75 Air Plan Apo Lambda DIC N220x | MRD00205 | 1.0 .75 | Air | Plan Apo Lambda | TBD | | >80% [400-950] | BF, Pol, DIC, Fluo | 0.17 |
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FiltersFilter cubes DAPI /Hoechst/AMCA cubesFITC/EGFP cubw TRITC/Rhodamine DAPI/FITC/TRITC/Cy5 - DIC Analyser
- CFP\YFP\mCherry
6 positions motorized - Ex 383-408 DAPI-U DM 425 BA 435-485
- Semrock 96372 M349727 17
- Semrock 96376 M351081 8
- DAPI/FITC/TRITC/Cy5 77074160 Custom Quad C182279 Polychroic and quad bandpassing single bandpass filters: ET395/25x, ET470/24x, ET550/15x, ET640/30x
- Ti2CDICACL
- CFP\YFP\mCherry7707\4656 CFP\YFP\mCherry XT C197767
Développer |
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| title | Filters complete specifications |
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| Position | Cube name | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter |
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1 | DAPI/Hoechst/AMCA | Chroma | Cube set 31000v2 | 350/50x [325-375] | 400LP | 460/50m [435-485] | 2 | FITC/EGFP | Chroma | Cube set 41001 | 480/40x [420-500] | 505LP | 535/50m [510-560] | 3 | TRITC/Rhodamine | Chroma | Cube set 41002c | 545/30x [530-560] | 570LP | 620/60m [590-650] | GFP/FITC (CFP) Cy5 DAPI/GFP/Cy3/Cy5 (requires Cy3 filter in Lumencor SpectraX Green/Yellow position) - DIC Analyzer
CFP/YFP/mCherry (requires mCherry filter in Lumencor SpectraX Green/Yellow position)
Développer |
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title | Filters complete specifications |
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Position | Name | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter | Comments |
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1 | DAPI | Nikon | DAPI-U HQ | 395/25x [383-408] | 425LP | 460/50m [435-485] | C-FL-C DAPI-U HQ | 2 | GFP | Semrock | GFP-4050B-000 | 466/40x [446-486] | 495LP | 525/50m
[500-550] | Nikon ID 96372 | 3 | Cy5 | Semrock | Cy5-5070A | 617/55x [590-645] | 652LP | 697/77m [659-736] | Nikon ID 96376 | 4 | DAPI/GFP/Cy3/Cy5 | Semrock | C182279 | None | 409/493/573/652 | 432/515/595/681 | 77074160 Custom Quad C182279. Excitation filters are in the Lumencor SpectraX light source | 5 | DIC Analyzer | Nikon | Ti2-C-DICACL | Not Applicable | Not Applicable | Not Applicable | Used for DIC imaging | 6 | CFP\YFP\mCherry | Semrock | C1997767 | None | 459/526/596 | 475/543/702 | Excitation filters are in the Lumencor SpectraX light source |
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4 | Texas Red | Chroma | Cube set 41004 | 560/55x [532-588] | 595LP | 645/75m [607-682] | 5 | Empty | 6 | Empty |
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id | User Guide |
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title | User Guide |
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| - Turn on the computer (#1)
- Remove the cover from the microscope
- Turn on the microscope power bar (#2)
If incubation is required, turn on the Okolab incubation module (3A and 3B) #3A), the Lauda water bath (#3B) and open the CO2 (3C#3C) and N2 (3D#3D) tanks Avertissement |
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Make sure the humidifier and the water bath is are clean and properly filled with distilled water |
- Log in Windows using your UdM credentials
- Use your UdeM credentials to log in to Windows
- Start the software Start-up NIS-Elements
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The first time you log in the computeruse the instrument, you need to import the microscope settings into the software. To do this follow the instructions Setting-up NIS-Elementsinstructions First use protocol. |
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| - Save your data
- Close NIS-Elements software
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
- Get your samples from the microscope
- Turn off the computer
- If oil objectives were used, clean it Clean the oil objectives with lens cleaner and lens paper (not Kimwipes)
- If incubation was used, turn off the Okolab incubation module (3A and 3B#3A),Lauda water bath (#3B) and close the CO2 (3C#3C) and N2 (3D#3D) tanks
- Turn Wait until the SpectaX has cooled down and turn off the microscope power bar (2#2)
- Cover the microscope
Remarque |
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| - Take back Collect your samples including ones , especially those in the microscope
- Leave the microscope and the working area workspace clean
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title | Storage Managementmanagement |
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| - Files can be saved temporarily (during acquisition) on the to local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a one folder per laboratory using the principal investigator's last name. WithinInside, create one a folder per user (Firstname_Lastnameusing the following nomenclature (First Name_Last Name).
Remarque |
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| In any case, do not store your files should be removed from on the C: drive. |
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title | Setting-up NIS-ElementsFirst use |
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| Setting-up NIS-Elements | When using the microscope for Setting-up NIS-Elements | This process is required the first time you are using the instrument, you need to import the microscope settings into the software. You will usually do it this during the training session. It This procedure can also be performed if something is not working properly right or and if you want to refresh reset the software interfaceto its original settings. Remarque |
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This process will delete all experiment protocols and restore the parameters for the reset the software to the original settings for this specific microscope. |
- Close If open, close NIS-Elements
- Wait until the complete closure of NIS-Elements
- Open the folder Desktop\Logiciels
- and wait until it is completely closed (up to 30 seconds)
- On your Desktop open the Softwares folder
- Open Open the software NIS Settings Utility
- Click on the Import tab
- Click on Browse
- Navigate to your desktop Desktop
- Select the file Nikon-Ti2 Settings.bin
- Click Select
- Select all items
- Click Import
- Click OK
- Close the NIS Settings
- Open You can now reopen NIS-Elements
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id | LightpathLight path |
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title | Light path |
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| The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.
Light pathDownload the schematics to follow Transmitted light (Bright field, Phase Contrast, Polarized light) and Reflected light (fluorescence) paths. Light path schematics |
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ManualsAvailable manuals |
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| - Adjust focus drive jitter
- Add a holder for polarized light analyzer
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| - Nikon Preventive Maintenance
- Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
- 100x slightly damaged but not the lens
- Fluorescence cubes damaged: FITC TexasRed
- Focus drive has a jitter
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- Check condenser filters if labels match
- Check why 20x/0.75 DIC objective has no DIC prism
- Condenser DIC prism is DIC N1 while objective requires N2
- Check why at 4x Condenser gives a black shadow on the righ side of the image
- Adjust Objective XYZ offset
- Check plane because FOV is not flat
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| - Replacement mercury lamp bulb HBO 1003W/2
- 256 GB SSD added in workstation for OS
- Windows 10 Installation
- BIOS updated to v2.47
- Camera Firmware updated to v2.11
- NIS-Elements Basic Research v4.6 64-bits installed
- Creation NIS Elements Settings
- FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK
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id | Technical Datasheet |
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title | Technical Datasheet |
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| Stand- Nikon Ti2-E inverted Serial 540156 System 170110-Sys-006287
Light sources- Transmitted LED light
- ND32 filter
- Manual Polarizer
- Lumencor SpectraX 6-NII-SE Serial 9409 Filters B G R NT V
R 640\30-25 - G 470\24-25
- B 440\20-25
- NT 510\25-25
- V 395\25-25
- GY : 550\15-25
- Storage GY 575\25-25
CondenserObjectives- 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
- 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
- 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
- Empty
- Empty
- Empty
Développer |
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title | Objectives complete specifications |
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| Position | Name | Brand | ID | Magnification | Numerical Aperture | Immersion | Type | Working distance (mm) | Transmittance (% [nm]) | Technique | Cover glass thickness (mm) |
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1 | 10x/0.3 Air | Nikon | 10x/0.3 Air Plan Fluor Ph1 DLL | 10x | 0.3 | Air | Plan Fluor | 16 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 | 2 | 40x/0.75 Air | Nikon | 40x/0.75 Air Plan Fluor Ph2 DLL | 40x | 0.75 | Air | Plan Fluor | 0.72 | >80% [400-750] | BF, Pol, PhC, Fluo | 0.17 | 3 | | Nikon | 100x/1.3 Oil Plan Fluor Ph3 DLL | 100x | 1.3 | Plan Fluor | 0.2 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 | 4 | Empty | 5 | Empty | 6 | Empty | Stage- Motorized stage Ti2 SHU compatible Serial 127808
- Remote control joystick Ti2-S-JS Serial 127976
- Inserts
- Combo slide and 3cm dish with tilt adjustment insert
- Multiwell plate insert
Filters- Ex 383-408 DAPI-U DM 425 BA 435-485
- Semrock 96372 M349727 17
- Semrock 96376 M351081 8
- 77074160 Custom Quad C182279 Polychroic and quad bandpass emitter for use with the following single bandpass filters: ET395/25x, ET470/24x, ET550/15x, ET640/30x
- DIC Analyser Ti2CDICACL
- 7707\4656 CFP\YFP\mCherry XT C197767
Développer |
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title | Filters complete specifications |
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| Position | Cube name | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter |
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1 | DAPI/Hoechst/AMCA | Chroma | Cube set 31000v2 | 350/50x [325-375] | 400LP | 460/50m [435-485] | 2 | FITC/EGFP | Chroma | Cube set 41001 | 480/40x [420-500] | 505LP | 535/50m [510-560] | 3 | TRITC/Rhodamine | Chroma | Cube set 41002c | 545/30x [530-560] | 570LP | 620/60m [590-650] | 4 | Texas Red | Chroma | Cube set 41004 | 560/55x [532-588] | 595LP | 645/75m [607-682] | 5 | Empty | 6 | Empty | DetectorWorkstation- HP Z440 Workstation
- Intel Xeon E5-1630 v3 @ 3.7GHz
- RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
- OS 256GB SSD 550 MBs
- 2TB HD Data Storage 150 MBs
- Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
- Monitor HP Z24i display 24' 1920x1200
Incubation- Okolab BoldLine Temperature unit Serial 284-1058 H101 T Unit BL
- Okolab BoldLine CO2/O2 Unit 0-10/1-18 Serial 088-1102
- Okolab OkoTouch Serial 118-224
Consumables Tabs Page |
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id | FAQ |
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title | Troubleshooting & FAQ |
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| UI Expand |
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expanded | true |
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title | Troubleshooting |
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| Liquid forming in the incubation chamber This happens when the mixed-gas humidifier is overfilled. Bubbles created by the gas going through the humidification bring liquid into the gas feed line. - Turn off the Okolab module
- Remove your sample and store it properly
- Dry the incubation chamber with a clean tissue
- Carefully remove the cap of the humidifier glass bottle
Avertissement |
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The humidifier bottle is made of glass and is very fragile. Pay extra care when manipulating the humidifier bottle |
- Remove distilled water from the humidifier
Info |
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Humidifier shouldn't be more than 2/3rd filled |
- Close the humidifier by replacing the cap
- Turn on the Okolab module
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UI Expand |
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| Can I use this microscope to look at cell in a dish? - Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate as well as slides between a glass slide and a 0.17mm thick cover slip.
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