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icon	time
id	Booking
title	Booking
type	standard
url	https://faces.ccrc.uga.edu/login0.php?account=Microscopie_UdeM
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Tabs Container

id	Instrument Tabs
title	Nikon Eclipse E600
direction	horizontal

Tabs Page

id	Description
title	Description

Nikon Eclipse E-600 upright microscope

Roger Gaudry Building, Room N-620
Simple Microscope usage price

Applications
- BrightfieldBright-field
- Phase Contrast
- Polarized light
- Fluorescence
- Color camera
Light sources
- Halogen lamp for 30 W transmitted light
- Mercury lamp (350~600nm) for fluorescence
- Lumencor SOLA V-nIR

Développer

title	Lumencor SOLA V-nIR complete specifications

Emission peak (nm)	Power (mW)
395	40
430	35
475	15
510	20
550	20
580	20
640	20
730	10

Lumencor SOLA manual.pdf
Lumencor SOLA datasheet.pdf
Lumencor SOLA recommended filter sets.pdf

Objectives
- 4x/0.13 Air WD 17.1
Objectives:
- 10x/0.3 Air Ph1 WD 16.0
- 20x/0.5 Air DIC 2.1
- 40x/0.75 Air Ph2 WD 0.72
- 60x/0.85 Air WD 0.3
- 100x/1.3 Oil Ph3 WD 0.2

Développer

title	Detailed Objectives complete specifications

Position

Name

Brand

ID

MagnificationFull name

Numerical Aperture

Immersion

Type

Working distance (mm)

Transmittance
(% [nm])

Technique

Cover glass thickness (mm)

1

4x/0.13 Air

Nikon

4x/0.3 Air Plan Fluor

0.13

Air

Plan Fluor

17.1

BF, Fluo

-

2

10x/0.3 Air

Nikon

10x/0.3 Air Plan Fluor Ph1 DLL

10x

0.3

Air

Plan Fluor

16

>75% [400-800]
Max % @500nm

BF, Pol, PhC, Fluo

0.17

3

20x/0.5

Nikon

20x/0.5 Air Plan Fluor DIC M

0.5

Air

Plan Fluor

2.1

BF, Fluo

0.17

4

40x/0.75 Air

Nikon

40x/0.75 Air Plan Fluor Ph2 DLL

40x

0.75

Air

Plan Fluor

0.72

>80% [400-750]
Max % @500nm

BF, Pol, PhC, Fluo

0.17

5

60x/0.85 Air

Nikon

60x/0.85 Air Plan Fluor

0.85

Air

Plan Fluor

0.30

BF, Fluo

Ajustable 0.11-0.23

63

100x/1.3 Oil Oil

Nikon

100x/1.3 Oil Plan Fluor Ph3 DLL

100x

1.3

Remarque
Oil

Plan Fluor

0.2

>75% [400-800]
Max % @500nm

BF, Pol, PhC, Fluo

0.17

4

Empty

5

Empty

6

Empty

Filter cubes
- DAPI/Hoechst/AMCA
- FITC/EGFP
- TRITC/Rhodamine
- TexasRed
- Empty
- Empty

Développer

title	Detailed Filters complete specifications

Position	Cube nameName	Brand	ID	Excitation Filter	Dichroic mirror	Emission Filter
1	DAPI/Hoechst/AMCA	Chroma	Cube set 31000v2	350/50x [325-375]	400LP	460/50m [435-485]
2	FITC/EGFP	Chroma	Cube set 41001	480/40x [420-500]	505LP	535/50m [510-560]
3	TRITC/Rhodamine	Chroma	Cube set 41002c	545/30x [530-560]	570LP	620/60m [590-650]
4	Texas Red	Chroma	Cube set 41004	560/55x [532-588]	595LP	645/75m [607-682]
5	Empty
6	Empty

Detector
- Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6fps 6 images/s at full frame

Tabs Page

id	User Guide
title	User Guide

halogen lamp on the right side of the microscope (#3)

Log in Windows using your UdM credentials

Start-up NIS-Elements

UI Expand

expanded	true
title	StartupStart-up

Turn on the computercomputer (#1)
Turn on the microscope power barbar (#2)
If fluorescence is required, press the mode button on the lumencor remote
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
If transmitted light is required, turn turn on the mercury lamp power supply unit (3A) and press ignition (3B)

Avertissement

Info
The first time you log in the computeruse the instrument, you need to import the microscope settings into the software. To do this follow the instructions Nikon Eclipse E600-enSoftware setup.

UI Expand

title	Shutdown

Save your data
Close NIS-Elements
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: driveGet your samples from the microscope
Turn off the computer
If oil objective was used, clean the oil objective it with lens cleaner and lens paperIf fluorescence was used, turn off the mercury lamp power supply unit (3A (not Kimwipes)
Turn off the microscope power bar (2#2)
Wait until the lamps are cool and cover the Cover the microscope

Remarque

title	Important Reminders

Take back your samples including ones in the microscope
Leave the microscope and the working area cleanMercury lamp must be on for at least 30 min before being turned off and vice-versa

UI Expand

title	Storage Managementmanagement

Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

Remarque
In any case, your files should be removed from the C: drive.

This process is required the first time you are using the instrument

. You will usually do

it

this during the training session.

It

This procedure can also be performed if something is not working properly

right or

and if you want to

refresh

reset the software

interface

to its original settings.

UI Expand

title	Setting-up NIS-Elements	Software setup

The first time you use the instrument, you need to import the microscope settings into the software

Ancre

Setting-up NIS-Elements

Remarque
This process will delete all experiment protocols and restore the parameters for the microscopereset the software to the original settings for this specific microscope.

If open, close NIS-Elements and wait until it is completely closed (up to 30 seconds)
On your Desktop open the Softwares folder
Open
Close NIS-Elements
Wait until the complete closure of NIS-Elements
Open the folder Desktop\Logiciels
Open the software NIS Settings Utility
Click on the Import tab
Click on Browse
Navigate to your desktop Desktop
Select the file Nikon-E600 Settings.bin
Click Select
Select all items
Click Import
Click OK
Close the NIS Settings
Open You can now reopen NIS-Elements

400

Tabs Page

id	Lightpath
title	Lightpath

Follow the schematics below for Transmitted light (Bright

The following schematics depict the light path for transmitted (bright-field, Phase Contrast, Polarized light) and

transmitted light

reflected (fluorescence) lights.

view-file

PDF

name	LightPath_Nikon E600.pdf

height

Tabs Page

id	Manuals
title	Manuals

Available manuals

Nikon

_

Eclipse

-

E600

_

Manual

.

_

Eclipse

-

E600

_

Polarization

_

Manual

.

_

Eclipse

-

E600

_

Epi Fluo D

_

Manual

.

_

Eclipse

-

E600

_

Epi Fluo Y

_

Manual

.

_

Camera DS-Ri2

_

Manual

.

_

BR

_

Manual

.

(pdf)

Tabs Page

id	Log
title	Log

UI Expand

expanded	true
title	To do

Adjust focus drive jitter
Add a holder for polarized light analyzer
Bring new test slides

UI Expand

expanded	true
title	2022-09-26

Added Lumencor SOLA v-nIR
Added Fluorescent light collimator

UI Expand

expanded	true
title	2022-09-26

Added 4x/0.13 Air
Added 20x/0.5 Air
Added 60x/0.85 Air
Updated E600 settings file

UI Expand

title	2021-07-20

Replacement mercury lamp bulb HBO 1003W/2
256 GB SSD added in workstation for OS
Windows 10 Installation
BIOS updated to v2.47
Camera Firmware updated to v2.11
NIS-Elements Basic Research v4.6 64-bits installed
Creation NIS Elements Settings
FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK

UI Expand

title	2018-04-04

Nikon Preventive Maintenance
Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
100x slightly damaged but not the lens
Fluorescence cubes damaged: FITC

TxRed

, TexasRed
Focus drive has a

jutter

jitter

Manual Stage

Filters

DAPI/Hoechst/AMCA

350/50x

FITC/EGFP

Cube set 41001

TRITC/Rhodamine

TexasRed

Detector

Color

Detector

Camera Nikon DS-Ri2 Serial 701234

Workstation

HP Z440 Workstation
Intel Xeon E5-1630 v3 @ 3.7GHz
RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
OS

256GB

256 GB SSD 550 MBs

2TB

2 TB HD Data Storage 150 MBs
Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
Monitor HP Z24i display 24'

1920x1200

1920 x 1200

Consumables

Mercury lamp bulb OSRAM HBO 1003W/2 100W

Tungsten Halogen Lamp OSRAM 64623 HLX 100W
12V
12V
3mm liquid light guide (Thorlabs LLG3-4Z)

Tabs Page

id	Technical Datasheet
title	Technical Datasheet

Stand

Nikon Eclipse

E600

E600W upright Serial 725540

Light sources

Transmitted light Halogen 12V 100W

Serial

Model C-LPSerial 01875599
Reflected light
Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
Bulb housing model LH M100C-1 Serial 039326
- - Lumencor SOLA v-nIR Serial 27517
  - 3mm liquid light guide (Thorlabs LLG3-4Z)
Condenser
- Condenser Universal C-CU Serial 081901
- Lens Dry NA 0.9
- Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty
Objectives
- 4x/0.13
- 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
- 20x/
1
- 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air
(Transmission curve)
- with PF40 Wollaston prism
- 60x/0.85
- 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil
(Transmission curve)
- with PF/PA 100 Oil Wollaston prism
Empty
Stage
Empty
Empty
Turret

Position

Cube name

Brand

ID

Excitation Filter

Dichroic mirror

Emission Filter

1

Chroma

Cube set 31000v2

400LP

460/50m

2

Chroma

480/40x

505LP

535/50m

3

Chroma

4

Texas Red

Chroma

5

Empty

6

Empty

Tabs Page

id	FAQ
title	Troubleshooting & FAQ

Troubleshooting

UI Expand

title	NIS-Elements shows an error: Camera Driver...

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

Turn off NIS-Elements
Turn on the camera (both the microscope power bar and the camera need to be ON
(the camera is usually ON, the switch on the top of the camera)
Check the camera is correctly connected to the computer via a USB cable
Turn on NIS-Elements

FAQ

UI Expand

title	Can I use this microscope to look at cells in a dish?

No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.

Nikon Eclipse E-600

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id	Demo Image

Tabs Container

id	Instrument Tabs
title	Nikon Eclipse E600
direction	horizontal

Tabs Page

id

direction

title	Nikon Eclipse E-600

horizontal

Tabs Page

id	Demo Image
title	Demo Image

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Image Added

Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)
Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)

Tabs Page

id	Nikon Eclipse E-600 at Roger Gaudry N-620
title	N-620

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Inclure page

	Plateformes:_foot-en
	Plateformes:_foot-en

Last modified by on

Raccourcis espace

Arborescence des pages

Comparaison des versions

Ancienne version 109

Nouvelle version Actuel

Légende

Nikon Eclipse E-600 upright microscope

The following schematics depict the light path for transmitted (bright-field, Phase Contrast, Polarized light) and

reflected (fluorescence) lights.

Filters

Detector

Workstation

Consumables

Tungsten Halogen Lamp OSRAM 64623 HLX 100W
12V
12V
3mm liquid light guide (Thorlabs LLG3-4Z)

Stand

Light sources

Condenser

Objectives

4x/0.13
10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
20x/
1
40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air
(Transmission curve)
with PF40 Wollaston prism
60x/0.85
100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil
(Transmission curve)
with PF/PA 100 Oil Wollaston prism
Empty

Stage

Troubleshooting

FAQ

Raccourcis espace

Arborescence des pages

Historique de la page

Comparaison des versions

Ancienne version 109

Nouvelle version Actuel

Légende

Nikon Eclipse E-600 upright microscope

The following schematics depict the light path for transmitted (bright-field, Phase Contrast, Polarized light) and

reflected (fluorescence) lights.

Stand

Light sources

Condenser

Objectives

4x/0.1310x/0.3 Plan Fluor Ph1 DLL WD 16 Air20x/140x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air (Transmission curve) with PF40 Wollaston prism60x/0.85100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil (Transmission curve) with PF/PA 100 Oil Wollaston prismEmpty

Stage

Filters

Detector

Workstation

Consumables

Tungsten Halogen Lamp OSRAM 64623 HLX 100W 12V 12V3mm liquid light guide (Thorlabs LLG3-4Z)

Troubleshooting

FAQ

4x/0.13
10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
20x/
1
40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air
(Transmission curve)
with PF40 Wollaston prism
60x/0.85
100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil
(Transmission curve)
with PF/PA 100 Oil Wollaston prism
Empty

Tungsten Halogen Lamp OSRAM 64623 HLX 100W
12V
12V
3mm liquid light guide (Thorlabs LLG3-4Z)