...
Another advantage of the calibo1p1 program is that you can assess the quality of your sample by looking at the 1D proton spectrum it produces (see above). Well dispersed peaks, particularly in the amide protons region (6-10 ppm) are indicative of a diverse chemical environment around these hydrogen atoms, which typically correlates with the presence of structural elements.
Data acquisition
Once everything is locked, tuned, matched, shimmed and calibrated for optimal signal intensity and quality, it is time to load a new experiment. In the case of a 1D proton experiment, the default acquisition parameters for a protein sample are saved as PROT_1DESNIG. To load them in a new experiment, first create a new experiment using the new command. In the dialog box, select the acquisition parameters PROT_1DESNIG s