Tabs Container

id	Instrument Tabs
title	Nikon Eclipse E600
direction	horizontal

Tabs Page

id	Description
title	Description

Nikon Eclipse E-600 upright microscope

Roger Gaudry Building, Room N-620
Simple Microscope usage price

Applications
- BrightfieldBright-field
- Phase Contrast
- Polarized light
- Fluorescence
- Color camera
Light sources
- Halogen lamp for 30 W transmitted light
- Mercury lamp (350~600nm) for fluorescence
- Lumencor SOLA V-nIR

Développer

title	Lumencor SOLA V-nIR complete specifications

Emission peak (nm)	Power (mW)
395	40
430	35
475	15
510	20
550	20
580	20
640	20
730	10

Lumencor SOLA manual.pdf
Lumencor SOLA datasheet.pdf
Lumencor SOLA recommended filter sets.pdf

Objectives
Objectives
- 4x/0.13 Air WD 17.1
- 10x/0.3 Air Ph1 WD 16.0
- 20x/0.5 Air DIC 2.1
- 40x/0.75 Air Ph2 WD Ph2 WD 0.72
- 60x/0.85 Air WD 0.753
- 100x/1.3 Oil Ph3 WD 0.2

Développer

title	Objectives complete specifications

Position

Name

Brand

ID

MagnificationFull name

Numerical Aperture

Immersion

Type

Working distance (mm)

Transmittance
(% [nm])

Technique

Cover glass thickness (mm)

1

4x/0.13 Air

Nikon

4x/0.3 Air Plan Fluor

0.13

Air

Plan Fluor

17.1

BF, Fluo

-

2

10x/0.3 Air

Nikon

10x/0.3 Air Plan Fluor Ph1 DLL

10x

0.3

Air

Plan Fluor

16

>75% [400-800]
Max % @500nm

BF, Pol, PhC, Fluo

0.17

3

20x/0.5

Nikon

20x/0.5 Air Plan Fluor DIC M

0.5

Air

Plan Fluor

2.1

BF, Fluo

0.17

4

40x/0.75 Air

Nikon

40x/0.75 Air Plan Fluor Ph2 DLL

40x

0.75

Air

Plan Fluor

0.72

>80% [400-750]
Max % @500nm

BF, Pol, PhC, Fluo

0.17

5

60x/0.85 Air

Nikon

60x/0.85 Air Plan Fluor

0.85

Air

Plan Fluor

0.30

BF, Fluo

Ajustable 0.11-0.23

63

100x/1.3 Oil Oil

Nikon

100x/1.3 Oil Plan Fluor Ph3 DLL

100x

1.3

Remarque
Oil

Plan Fluor

0.2

>75% [400-800]
Max % @500nm

BF, Pol, PhC, Fluo

0.17

4

Empty

5

Empty

6

Empty

Filter cubes
- DAPI/Hoechst/AMCA
- FITC/EGFP
- TRITC/Rhodamine
- TexasRed
- Empty
- Empty

Développer

title	Filters complete specifications

Position	Cube nameName	Brand	ID	Excitation Filter	Dichroic mirror	Emission Filter
1	DAPI/Hoechst/AMCA	Chroma	Cube set 31000v2	350/50x [325-375]	400LP	460/50m [435-485]
2	FITC/EGFP	Chroma	Cube set 41001	480/40x [420-500]	505LP	535/50m [510-560]
3	TRITC/Rhodamine	Chroma	Cube set 41002c	545/30x [530-560]	570LP	620/60m [590-650]
4	Texas Red	Chroma	Cube set 41004	560/55x [532-588]	595LP	645/75m [607-682]
5	Empty
6	Empty

Detector
- Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6fps 6 images/s at full frame

Tabs Page

id	User Guide
title	User Guide

UI Expand

expanded	true
title	StartupStart-up

Turn on the computercomputer (#1)
Turn on the microscope power barbar (#2)
If fluorescence is required, turn press the mode button on the lumencor remote
If transmitted light is required, turn on the mercury lamp power supply unit (3A) and press ignition (3B)
Avertissement
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
halogen lamp on the right side of the microscope (#3)
Log in Windows using your UdM credentials
Start-up NIS-Elements

Info
The first time you log in the computeruse the instrument, you need to import the microscope settings into the software. To do this follow the instructions Setting-up NIS-ElementsSoftware setup.

UI Expand

title	Shutdown

Save your data
Close NIS-Elements
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: driveGet your samples from the microscope
Turn off the computer
If oil objective was used, clean the oil objective it with lens cleaner and lens paperIf fluorescence was used, turn off the mercury lamp power supply unit (3A (not Kimwipes)
Turn off the microscope power bar (2#2)
Wait until the lamps are cool and cover the Cover the microscope

Remarque

title	Important Reminders

Take back your samples including ones in the microscope
Leave the microscope and the working area cleanMercury lamp must be on for at least 30 min before being turned off and vice-versa

UI Expand

title	Storage Managementmanagement

Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

Remarque
In any case, your files should be removed from the C: drive.

This process is required the first time you are using the instrument

. You will usually do

it

this during the training session.

It

This procedure can also be performed if something is not working properly

right or

and if you want to

refresh

reset the software

interface

to its original settings.

UI Expand

title	Setting-up NIS-Elements	Software setup

The first time you use the instrument, you need to import the microscope settings into the software

Ancre

Setting-up NIS-Elements

Remarque
This process will delete all experiment protocols and restore the parameters for the reset the software to the original settings for this specific microscope.

Close If open, close NIS-Elements
Wait until the complete closure of NIS-Elements
Open the folder Desktop\Logiciels
and wait until it is completely closed (up to 30 seconds)
On your Desktop open the Softwares folder
Open Open the software NIS Settings Utility
Click on the Import tab
Click on Browse
Navigate to your desktop Desktop
Select the file Nikon-E600 Settings.bin
Click Select
Select all items
Click Import
Click OK
Close the NIS Settings
Open You can now reopen NIS-Elements

Tabs Page

id	Lightpath
title	Light path	Lightpath

The following schematics depict the light path for transmitted (bright-

Light path

Download the schematics to follow Transmitted light (Bright

field, Phase Contrast, Polarized light) and

Reflected light

reflected (fluorescence)

paths

lights.

PDF

name	LightPath_Nikon E600

Light path schematics

.pdf

Tabs Page

id	Manuals
title	Manuals

Manuals

Available manuals

Tabs Page

id	Log
title	Log

UI Expand

expanded	true
title	To do

Adjust focus drive jitter
Add a holder for polarized light analyzer
Bring new test slides

UI Expand

expanded	true
title	2018-04-04		2022-09-26

Added Lumencor SOLA v-nIR
Added Fluorescent light collimator

UI Expand

expanded	true
title	2022-09-26

Added 4x/0.13 Air
Added 20x/0.5 Air
Added 60x/0.85 Air
Updated E600 settings file
Nikon Preventive Maintenance
Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
100x slightly damaged but not the lens
Fluorescence cubes damaged: FITC TexasRed
Focus drive has a jitter

UI Expand

title	2021-07-20

Replacement mercury lamp bulb HBO 1003W/2
256 GB SSD added in workstation for OS
Windows 10 Installation
BIOS updated to v2.47
Camera Firmware updated to v2.11
NIS-Elements Basic Research v4.6 64-bits installed
Creation NIS Elements Settings
FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK

UI Expand

title	2018-04-04

Nikon Preventive Maintenance
Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
100x slightly damaged but not the lens
Fluorescence cubes damaged: FITC, TexasRed
Focus drive has a jitter

Stage

Manual Stage

Tabs Page

id	Technical Datasheet
title	Technical Datasheet

Stand

Nikon Eclipse E600 E600W upright Serial 725540

Light sources

Transmitted light Halogen 12V 100W Serial Model C-LPSerial 01875599
Reflected light
- Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
- Lumencor SOLA v-nIR Serial 27517
- 3mm liquid light guide (Thorlabs LLG3-4Z)Bulb housing model LH M100C-1 Serial 039326

Condenser

Condenser Universal C-CU Serial 081901
Lens Dry NA 0.9
Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty

Objectives

4x/0.13
10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
20x/
40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
60x/0.85
100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
Empty
Empty
Empty

Développer

title	Objectives complete specifications

Position

Name

Brand

ID

Magnification

Numerical Aperture

Immersion

Type

Working distance (mm)

Transmittance
(% [nm])

Technique

Cover glass thickness (mm)

1

10x/0.3 Air

Nikon

10x/0.3 Air Plan Fluor Ph1 DLL

10x

0.3

Air

Plan Fluor

16

>75% [400-800]

BF, Pol, PhC, Fluo

0.17

2

40x/0.75 Air

Nikon

40x/0.75 Air Plan Fluor Ph2 DLL

40x

0.75

Air

Plan Fluor

0.72

>80% [400-750]

BF, Pol, PhC, Fluo

0.17

3

100x/1.3 Oil

Nikon

100x/1.3 Oil Plan Fluor Ph3 DLL

100x

1.3

Remarque
Oil

Plan Fluor0.2>75% [400-800]BF, Pol, PhC, Fluo0.174Empty5Empty6Empty

Filters

DAPI/Hoechst/AMCA
FITC/EGFP
TRITC/Rhodamine
TexasRed

Développer

title	Filters complete specifications

PositionCube nameBrandID

Excitation FilterDichroic mirrorEmission Filter1DAPI/Hoechst/AMCAChromaCube set 31000v2 350/50x [325-375]400LP 460/50m [435-485]2FITC/EGFPChromaCube set 41001

480/40x [420-500]

505LP

535/50m [510-560]3TRITC/RhodamineChroma

Cube set 41002c

545/30x [530-560]570LP 620/60m [590-650]4Texas RedChroma

Cube set 41004

560/55x [532-588]

595LP 645/75m [607-682]5Empty6Empty

Detector

Color Camera Nikon DS-Ri2 Serial 701234

Workstation

HP Z440 Workstation
Intel Xeon E5-1630 v3 @ 3.7GHz
RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
OS 256GB 256 GB SSD 550 MBs
2TB 2 TB HD Data Storage 150 MBs
Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
Monitor HP Z24i display 24' 1920x12001920 x 1200

Consumables

Mercury lamp bulb OSRAM HBO 1003W/2 100W
Tungsten Halogen Lamp OSRAM 64623 HLX 100W 12V 12V
3mm liquid light guide (Thorlabs LLG3-4Z)

Tabs Page

id	FAQ
title	Troubleshooting & FAQ

Troubleshooting

true

UI Expand

expanded

Troubleshooting

title		NIS-Elements shows an error: Camera Driver...

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

Turn off NIS-Elements
Turn on the camera (both the microscope power bar and the camera need to be ON
(the camera is usually ON, the switch on the top of the camera)
Check the USB conexion between the camera and is correctly connected to the computer via a USB cable
Turn on NIS-Elements

FAQ

cell

UI Expand

FAQ

title		Can I use this microscope to look at

cells in a dish?

No. This is an upright microscope designed to

look at

observe specimen mounted between a

glass

slide and a 0.

17mm

17 mm thick

cover slip

coverslip.

Tabs Container

id	Demo Image
title	Nikon Eclipse E-600
direction	horizontal

Tabs Page

id	Demo ImagesImage
title	Demo ImagesImage

Image Removed

Image Added

Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)
Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)

Inclure page

	Plateformes:_foot-en
	Plateformes:_foot-en

Raccourcis espace

Arborescence des pages

Comparaison des versions

Ancienne version 140

Nouvelle version Actuel

Légende

Nikon Eclipse E-600 upright microscope

The following schematics depict the light path for transmitted (bright-

Light path

field, Phase Contrast, Polarized light) and

reflected (fluorescence)

lights.

Stage

Stand

Light sources

Condenser

Objectives

Filters

Detector

Workstation

Consumables

Troubleshooting

FAQ

Raccourcis espace

Arborescence des pages

Historique de la page

Comparaison des versions

Ancienne version 140

Nouvelle version Actuel

Légende

Nikon Eclipse E-600 upright microscope

The following schematics depict the light path for transmitted (bright-

Light path

field, Phase Contrast, Polarized light) and

reflected (fluorescence)

lights.

Stand

Light sources

Condenser

Objectives

Stage

Filters

Detector

Workstation

Consumables

Troubleshooting

FAQ