Comparaison des versions

Ancienne version 147

changes.mady.by.user Nicolas Stifani

Sauvegardée le

comparé à

Nouvelle version Actuel

changes.mady.by.user Nicolas Stifani

Sauvegardée le

Légende

  • Ces lignes ont été ajoutées. Ce mot a été ajouté.
  • Ces lignes ont été supprimées. Ce mot a été supprimé.
  • La mise en forme a été modifiée.

Inclure page
_head-en
_head-en

Button Hyperlink
iconbuild
titleFrançais
typeprimary
urlhttps://wiki.umontreal.ca/display/Microscopie/Nikon+Eclipse+E600


Tabs Container
idInstrument Tabs
titleNikon Eclipse E600
directionhorizontal


Tabs Page
idDescription
titleDescription

Nikon Eclipse E-600 upright microscope

Roger Gaudry Building, Room N-620
Simple Microscope usage price

  • Applications

    • Bright-field

    • Phase Contrast

    • Polarized light

    • Fluorescence

    • Color camera

  • Light sources

    • Halogen lamp for 30 W transmitted lightMercury lamp 100 W (350~600nm) for fluorescence 

    • Lumencor SOLA V-nIR

Développer
titleMercury lamp Lumencor SOLA V-nIR complete specifications


Peak emission Emission peak (nm)Power (mW)
3347
36545
40534
43643
54637
57926

HBO Mercury lamp emission spectra (Source)
Comparison HBO Mercury vs XCite Metal Halide Lamps (Source)
OSRAM HBO 100W/2 Mercury lamp manual (pdf)

39540
43035
47515
51020
55020
58020
64020
73010

Lumencor SOLA manual.pdf
Lumencor SOLA datasheet.pdf
Lumencor SOLA recommended filter sets.pdf

  • Objectives

    Objectives

    • 4x/0.13 Air WD 17.1

    • 10x/0.3 Air Ph1 WD 16.0

    • 20x/0.5 Air DIC 2.1

    • 40x/0.75 Air Ph2 WD Ph2 WD 0.72

    • 60x/0.85 Air WD 0.753
    • 100x/1.3 Oil Ph3 WD 0.2
Développer
titleObjectives complete specifications


PositionNameBrandFull nameProduct IDMagnificationNumerical ApertureImmersionTypeWorking distance (mm)Transmittance
(% [nm])		TechniqueCover glass thickness (mm)
14x/0.13 AirNikon4x/0.3 Air Plan Fluor0.13AirPlan Fluor17.1


BF, Fluo-
210x/0.3 AirNikon10x/0.3 Air Plan Fluor Ph1 DLL10x0.3AirPlan Fluor16

>75% [400-800]
Max % @500nm

BF, Pol, PhC, Fluo0.17
3

20x/0.5

Nikon20x/0.5 Air Plan Fluor DIC M0.5

Air

Plan Fluor2.1
BF, Fluo0.17
4

40x/0.75 Air

Nikon40x/0.75 Air Plan Fluor Ph2 DLL40x

0.75

Air

Plan Fluor0.72>80% [400-750]
Max % @500nm		BF, Pol, PhC, Fluo0.17
560x/0.85 AirNikon60x/0.85 Air Plan Fluor0.85AirPlan Fluor0.30
BF, FluoAjustable 0.11-0.23
63

100x/1.3 Oil Oil

Nikon100x/1.3 Oil Plan Fluor Ph3 DLL

100x

1.3
Remarque
Oil

 

Plan Fluor0.2>75% [400-800]
Max % @500nm
BF, Pol, PhC, Fluo0.174Empty5Empty6Empty


  • Filter cubes

    • DAPI/Hoechst/AMCA

    • FITC/EGFP

    • TRITC/Rhodamine

    • TexasRed

    • Empty
    • Empty
Développer
titleFilters complete specifications


PositionNameBrandIDExcitation FilterDichroic mirrorEmission FilterComments
1DAPI/Hoechst/AMCAChromaCube set 31000v2350/50x
[325-375]		400LP460/50m
[435-485]
2FITC/EGFPChromaCube set 41001

480/40x
[420-500]

505LP

535/50m
[510-560]
3TRITC/RhodamineChroma

Cube set 41002c

545/30x
[530-560]		570LP620/60m
[590-650]
4Texas RedChroma

Cube set 41004

560/55x
[532-588]

595LP645/75m
[607-682]
5Empty





6Empty






  • Detector

    • Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6 images/s at full frame


Tabs Page
idUser Guide
titleUser Guide


UI Expand
expandedtrue
titleStartupStart-up
  1. Turn on the computer (#1)
  2. Turn on the microscope power bar (#2)

  3. If fluorescence is required, press the mode button on the lumencor remote

  4. If transmitted light is required, turn  turn on the mercury lamp power supply unit (#3A) and press ignition (#3B)

    Avertissement

    Mercury lamp must be on for at least 30 min before being turned off and vice-versa

    halogen lamp on the right side of the microscope (#3)

  5. Log in Windows using your UdM credentials

  6. Start-up NIS-Elements
Info

The first time you log in the computeruse the instrument, you need to import the microscope settings into the software. To do this follow the instructions Software setup.



UI Expand
titleShutdown
  1. Save your data
  2. Close NIS-Elements
  3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  4. Turn off the computer
  5. If oil objective was used, clean the oil objective it with lens cleaner and lens paperIf fluorescence was used, turn off the mercury lamp power supply unit (#3A (not Kimwipes)
  6. Turn off the microscope power bar (#2)
  7. Wait until the lamps have cooled and cover the Cover the microscope
Remarque
titleImportant Reminders
  • Take back your samples including ones in the microscope
  • Leave the microscope and the working area cleanMercury lamp must be on for at least 30 min before being turned off and vice-versa



UI Expand
titleStorage management
  • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from the local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
Remarque

In any case, your files should be removed from the C: drive.



UI Expand
titleSoftware setup
This process is required the

The first time

you are using the instrument

you use the instrument, you need to import the microscope settings into the software. You will usually do

it

this during the training session.

It


This procedure can also be performed if something is not working properly

or

and if you want to reset the software

interface

to its original settings.

Remarque

This process will delete all experiment protocols and restore reset the software to the original parameters settings for the this specific microscope.

  1. Close If open, close NIS-Elements
  2. Wait until the complete closure of NIS-Elements
  3. Open the folder Desktop\Logiciels
  4.  and wait until it is completely closed (up to 30 seconds)
  5. On your Desktop open the Softwares folder
  6. Open Open the software NIS Settings Utility
  7. Click on the Import tab
  8. Click on Browse
  9. Navigate to your desktop Desktop
  10. Select the file Nikon-E600 Settings.bin
  11. Click Select
  12. Select all items
  13. Click Import
  14. Click OK
  15. Close the NIS Settings
  16. Open You can now reopen NIS-Elements



Tabs Page
idLightpath
titleLight pathLightpath


The following schematics depict the light path for transmitted (bright-field, Phase Contrast, Polarized light) and reflected (fluorescence) lights.

PDF
nameLightPath_Nikon E600
Lightpath (
.pdf
)


Tabs Page
idManuals
titleManuals


Available manuals


Tabs Page
idLog
titleLog


UI Expand
expandedtrue
titleTo do
  • Adjust focus drive jitter
  • Add a holder for polarized light analyzer
  • Bring new test slides


UI Expand
expandedtrue
title2018-04-042022-09-26
  • Added Lumencor SOLA v-nIR
  • Added Fluorescent light collimator


UI Expand
expandedtrue
title2022-09-26
  • Added 4x/0.13 Air
  • Added 20x/0.5 Air
  • Added 60x/0.85 Air
  • Updated E600 settings file
  • Nikon Preventive Maintenance
  • Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
  • 100x slightly damaged but not the lens
  • Fluorescence cubes damaged: FITC TexasRed
  • Focus drive has a jitter


UI Expand
title2021-07-20
  • Replacement mercury lamp bulb HBO 1003W/2
  • 256 GB SSD added in workstation for OS
  • Windows 10 Installation
  • BIOS updated to v2.47
  • Camera Firmware updated to v2.11
  • NIS-Elements Basic Research v4.6 64-bits installed
  • Creation NIS Elements Settings
  • FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK


UI Expand
title2018-04-04
  • Nikon Preventive Maintenance
  • Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
  • 100x slightly damaged but not the lens
  • Fluorescence cubes damaged: FITC, TexasRed
  • Focus drive has a jitter




Tabs Page
idTechnical Datasheet
titleTechnical Datasheet

Stand

  • Nikon Eclipse E600 E600W upright Serial 725540

Light sources

  • Transmitted light Halogen 12V 100W Serial Model C-LPSerial 01875599
  • Reflected light

Condenser

  • Condenser Universal C-CU Serial 081901
  • Lens Dry NA 0.9
  • Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty

Objectives

  • 4x/0.13
  • 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
  • 20x/
  • 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
  • 60x/0.85
  • 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
  • Empty
  • Empty
  • Empty

Stage

  • Manual Stage TBD

Filters

  • DAPI/Hoechst/AMCA

  • FITC/EGFP

  • TRITC/Rhodamine

  • TexasRed

Detector

  • Color Camera Nikon DS-Ri2 Serial 701234

Workstation

  • HP Z440 Workstation
  • Intel Xeon E5-1630 v3 @ 3.7GHz
  • RAM 32 GB DDR4 2133 MHz  (4 x 8 GB)
  • OS 256 GB SSD 550 MBs
  • 2 TB HD Data Storage 150 MBs
  • Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
  • Monitor HP Z24i display 24' 1920 x 1200

Consumables


Tabs Page
idFAQ
titleTroubleshooting & FAQ

Troubleshooting

UI Expand
titleNIS-Elements shows an error: Camera Driver...

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

  • Turn off NIS-Elements
  • Turn on the camera (both the microscope power bar and the camera need to be ON
    (the camera is usually ON, the switch on the top of the camera)
  • Check the camera is correctly connected to the computer via a USB cable
  • Turn on NIS-Elements

FAQ

UI Expand
titleCan I use this microscope to look at cells in a dish?

No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.





Tabs Container
idDemo Image
titleNikon Eclipse E-600
directionhorizontal


Tabs Page
idDemo Image
titleDemo Image


Image AddedImage Removed

Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)
Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)




Inclure page
Plateformes:_foot-en
Plateformes:_foot-en