Nikon Eclipse E-600 upright microscope

Roger Gaudry Building, Room N-620
Simple Microscope usage price

• Applications

  • Bright-field

  • Phase Contrast

  • Polarized light

  • Fluorescence

  • Color camera

• Light sources

  • Halogen lamp for 30 W transmitted lightMercury lamp 100 W (350~600nm) for fluorescence 

  • Lumencor SOLA V-nIR

• Objectives

  Objectives

  • 4x/0.13 Air WD 17.1

  • 10x/0.3 Air Ph1 WD 16.0

  • 20x/0.5 Air DIC 2.1

  • 40x/0.75 Air Ph2 WD Ph2 WD 0.72

  • 60x/0.85 Air WD 0.753
  • 100x/1.3 Oil Ph3 WD 0.2

• Filter cubes

  • DAPI/Hoechst/AMCA

  • FITC/EGFP

  • TRITC/Rhodamine

  • TexasRed

  • Empty
  • Empty

• Detector

  • Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6 images/s at full frame

press the mode button on the lumencor remote

1. Save your data
2. Close NIS-Elements
3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
4. Turn off the computer
5. If oil objective was used, clean it with lens cleaner and lens paper (not Kimwipes)
6. If fluorescence was used, turn off the mercury lamp power supply unit (#3A)
7. If transmitted light was used, turn off the halogen lamp on the right side of the microscope (#4)
8. Turn off the microscope power bar (#2)
9. Wait until the lamps have cooled and cover Cover the microscope

• Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
• At the end of each session, copy your data to your external drive and delete it from the local C: drive
• You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

The first time you use the instrument, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

1. If open, close NIS-Elements and wait until it is completely closed (up to 30 seconds)
2. On your Desktop open the Softwares folder
3. Open NIS Settings Utility
4. Click on the Import tab
5. Click on Browse
6. Navigate to your Desktop
7. Select the file Nikon-E600 Settings.bin
8. Click Select
9. Select all items
10. Click Import
11. Click OK
12. Close the NIS Settings
13. You can now reopen NIS-Elements

• Adjust focus drive jitter
• Add a holder for polarized light analyzer
• Bring new test slides

• Added Lumencor SOLA v-nIR
• Added Fluorescent light collimator

• Replacement mercury lamp bulb HBO 1003W/2
• 256 GB SSD added in workstation for OS
• Windows 10 Installation
• BIOS updated to v2.47
• Camera Firmware updated to v2.11
• NIS-Elements Basic Research v4.6 64-bits installed
• Creation NIS Elements Settings
• FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

• Turn off NIS-Elements
• Turn on the camera (both the microscope power bar and the camera need to be ON
  (the camera is usually ON, the switch on the top of the camera)
• Check the camera is correctly connected to the computer via a USB cable
• Turn on NIS-Elements

No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.