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Button Hyperlink
iconbuild
titleFrançais
typeprimary
urlhttps://wiki.umontreal.ca/display/Microscopie/Nikon+Eclipse+E600


Tabs Container
idInstrument Tabs
titleNikon Eclipse E600
directionhorizontal


Tabs Page
idDescription
titleDescription

Nikon Eclipse E-600 upright microscope

Roger Gaudry Building, Room N-620

Upright

Simple Microscope usage price

  • Applications

Brightfield

    • Bright-field

    • Phase Contrast

    • Polarized light

    • Fluorescence

    • Color camera

  • Light sources

    • Halogen lamp for 30 W transmitted light

  • Mercury lamp (350~600nm) for fluorescence 

      • Lumencor SOLA V-nIR

    Développer
    titleLumencor SOLA V-nIR complete specifications


    Emission peak (nm)Power (mW)
    39540
    43035
    47515
    51020
    55020
    58020
    64020
    73010

    Lumencor SOLA manual.pdf
    Lumencor SOLA datasheet.pdf
    Lumencor SOLA recommended filter sets.pdf

    • Objectives

      • 4x/0.13 Air WD 17.1

    Objectives:

      • 10x/0.3 Air Ph1 WD 16.0

      • 20x/0.5 Air DIC 2.1

      • 40x/0.75 Air Ph2 WD 0.72

      • 60x/0.85 Air WD 0.3
      • 100x/1.3 Oil Ph3 WD 0.2
    Développer
    titleObjectives complete specifications


    PositionNameBrandFull nameNumerical ApertureImmersionTypeWorking distance (mm)Transmittance
    (% [nm])    		TechniqueCover glass thickness (mm)
    14x/0.13 AirNikon4x/0.3 Air Plan Fluor0.13AirPlan Fluor17.1


    		BF, Fluo-
    210x/0.3 AirNikon10x/0.3 Air Plan Fluor Ph1 DLL0.3AirPlan Fluor16

    >75% [400-800]
    Max % @500nm

    		BF, Pol, PhC, Fluo0.17
    3

    20x/0.5

    		Nikon20x/0.5 Air Plan Fluor DIC M0.5

    Air

    		Plan Fluor2.1
    		BF, Fluo0.17
    4

    40x/0.75 Air

    		Nikon40x/0.75 Air Plan Fluor Ph2 DLL

    0.75

    Air

    		Plan Fluor0.72>80% [400-750]
    Max % @500nm    		BF, Pol, PhC, Fluo0.17
    560x/0.85 AirNikon60x/0.85 Air Plan Fluor0.85AirPlan Fluor0.30
    		BF, FluoAjustable 0.11-0.23
    6

    100x/1.3 Oil

    		Nikon100x/1.3 Oil Plan Fluor Ph3 DLL1.3
    Remarque
    Oil

     

    		Plan Fluor0.2>75% [400-800]
    Max % @500nm
    		BF, Pol, PhC, Fluo0.17


    • Filter cubes

      • DAPI/Hoechst/AMCA

      • FITC/EGFP

      • TRITC

  • TxRed

    • Detector

    CMOS

      • /Rhodamine

      • TexasRed

      • Empty
      • Empty
    Développer
    titleFilters complete specifications


    PositionNameBrandIDExcitation FilterDichroic mirrorEmission FilterComments
    1DAPI/Hoechst/AMCAChromaCube set 31000v2350/50x
    [325-375]    		400LP460/50m
    [435-485]
    2FITC/EGFPChromaCube set 41001

    480/40x
    [420-500]

    505LP

    		535/50m
    [510-560]
    3TRITC/RhodamineChroma

    Cube set 41002c

    		545/30x
    [530-560]    		570LP620/60m
    [590-650]
    4Texas RedChroma

    Cube set 41004

    560/55x
    [532-588]

    		595LP645/75m
    [607-682]
    5Empty





    6Empty






    • Detector

      • Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit,

    6fps

      • 6 images/s at full frame

    Nikon Eclipse E600 MicroscopeImage Removed

    Image Removed


    Tabs Page
    idUser Guide
    titleUser Guide


    UI Expand
    expandedtrue
    titleStart-up
    Startup
    1. Turn on the
    computer
    1. computer (#1)
    2. Turn on the microscope power
    bar
    1. bar (#2)

    2. If fluorescence is required,

    turn

    1. press the mode button on the lumencor remote

    2. If transmitted light is required, turn on the

    mercury lamp power supply unit (3A) and press ignition (3B) AvertissementMercury lamp must be on for at least 30 min before being turned off and vice-versa

    1. halogen lamp on the right side of the microscope (#3)

    2. Log in Windows using your UdM credentials

    3. Start-up NIS-Elements
    Note:
    Info

    The first time you

    log in the computer

    use the instrument, you need to import the microscope settings into the software. To do this follow the

    instructions Setting-up NIS-Elements.

    instructions Software setup.



    UI Expand
    titleShutdown
    1. Save your data
    2. Close NIS-Elements
    3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
    Get your samples from the microscope
    1. Turn off the computer
    2. If oil objective was used, clean
    the oil objective
    1. it with lens cleaner and lens paper
    If fluorescence was used, turn off the mercury lamp power supply unit (3A
    1. (not Kimwipes)
    2. Turn off the microscope power bar (
    2
    1. #2)
    Wait until the lamps are cool and cover the microscope
    1. Cover the microscope
    Remarque
    titleImportant Reminders
    • Take back your samples including ones in the microscope
    • Leave the microscope and the working area clean
  • Mercury lamp must be on for at least 30 min before being turned off and vice-versa



    • UI Expand
    titleStorage management
    Storage Management
    • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
    • At the end of each session, copy your data to your external drive and delete it from the local C: drive
    • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
    Remarque

    In any case, your files should be removed from the C: drive.

    Setting-up NIS-Elements

    This is required the first time you are using the instrument



    UI Expand
    titleSoftware setup

    The first time you use the instrument, you need to import the microscope settings into the software. You will usually do

    it

    this during the training session.

    It


    This procedure can also be performed if something is not working properly

    right or

    and if you want to

    refresh

    reset the software

    interface
  • Close NIS-Elements
  • Wait until the complete closure of NIS-Elements
  • Open the folder Desktop\Logiciels
    • Open the software

    to its original settings.

    Remarque

    This process will delete all experiment protocols and

    restore the parameters for the microscope

    reset the software to the original settings for this specific microscope.

    1. If open, close NIS-Elements and wait until it is completely closed (up to 30 seconds)
    2. On your Desktop open the Softwares folder
    3. Open 
    1. NIS Settings Utility
    2. Click on the Import tab
    3. Click on Browse
    4. Navigate to your
    desktop
    1.  Desktop
    2. Select the file Nikon-E600 Settings.bin
    3. Click Select
    4. Select all items
    5. Click Import
    6. Click OK
    7. Close the NIS Settings
    8. You can now reopen NIS-Elements



    Tabs Page
    idLightpath
    titleLightpath
    Follow the schematics below for Transmitted light (Bright


    The following schematics depict the light path for transmitted (bright-field, Phase Contrast, Polarized light) and

    transmitted light

    reflected (fluorescence) lights.

    view-file

    PDF
    nameLightPath_Nikon E600.pdf

    height



    Tabs Page
    400
    idManuals
    view-file
    titleManuals


    Available manuals


    Tabs Page
    idLog
    titleLog


    UI Expand
    expandedtrue
    titleTo do
    • Adjust focus drive jitter
    • Add a holder for polarized light analyzer
    • Bring new test slides


    UI Expand
    expandedtrue
    title2022-09-26
    • Added Lumencor SOLA v-nIR
    • Added Fluorescent light collimator


    UI Expand
    expandedtrue
    title2022-09-26
    • Added 4x/0.13 Air
    • Added 20x/0.5 Air
    • Added 60x/0.85 Air
    • Updated E600 settings file


    UI Expand
    title2021-07-20
    • Replacement mercury lamp bulb HBO 1003W/2
    • 256 GB SSD added in workstation for OS
    • Windows 10 Installation
    • BIOS updated to v2.47
    • Camera Firmware updated to v2.11
    • NIS-Elements Basic Research v4.6 64-bits installed
    • Creation NIS Elements Settings
    • FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK


    UI Expand
    title2018-04-04
    • Nikon Preventive Maintenance
    • Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
    • 100x slightly damaged but not the lens
    • Fluorescence cubes damaged: FITC, TexasRed
    • Focus drive has a jitter




    Tabs Page
    idTechnical Datasheet
    titleTechnical Datasheet

    Stand

    • Nikon Eclipse E600W upright Serial 725540

    Light sources

    Condenser

    • Condenser Universal C-CU Serial 081901
    • Lens Dry NA 0.9
    • Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty

    Objectives

    • 4x/0.13
    • 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
    • 20x/
    • 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
    • 60x/0.85
    • 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism

    Stage

    • Manual Stage

    Filters

    • DAPI/Hoechst/AMCA

    • FITC/EGFP

    • TRITC/Rhodamine

    • TexasRed

    Detector

    • Color Camera Nikon DS-Ri2 Serial 701234

    Workstation

    • HP Z440 Workstation
    • Intel Xeon E5-1630 v3 @ 3.7GHz
    • RAM 32 GB DDR4 2133 MHz  (4 x 8 GB)
    • OS 256 GB SSD 550 MBs
    • 2 TB HD Data Storage 150 MBs
    • Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
    • Monitor HP Z24i display 24' 1920 x 1200

    Consumables


    Tabs Page
    idFAQ
    titleTroubleshooting & FAQ

    Troubleshooting

    UI Expand
    titleNIS-Elements shows an error: Camera Driver...

    This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

    • Turn off NIS-Elements
    • Turn on the camera (both the microscope power bar and the camera need to be ON
      (the camera is usually ON, the switch on the top of the camera)
    • Check the camera is correctly connected to the computer via a USB cable
    • Turn on NIS-Elements

    FAQ

    UI Expand
    titleCan I use this microscope to look at cells in a dish?

    No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.





    Tabs Container
    idDemo Image
    titleNikon Eclipse E-600
    directionhorizontal


    Tabs Page
    idDemo Image
    titleDemo Image


    Image Added

    Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)
    Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)




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    name2021-07-25 Issue Laser 488nm Microfluidic 2.txt
    height250
  • Nikon_Eclipse-E600_Manual.pdf
  • Nikon_Eclipse-E600_Polarization_Manual.pdf
  • Nikon_Eclipse-E600_Epi Fluo D_Manual.pdf
  • Nikon_Eclipse-E600_Epi Fluo Y_Manual.pdf
  • Nikon_Camera DS-Ri2_Manual.pdf
    • NIS-Elements_BR_Manual.pdf