There is no single answer - If you have a single plan you should choose 2D-deconvolution
- If you have a Z-stack you should pick 3D-deconvolution.
- If you want to have a quick view of the result you should use Fast Deconvolution.
Quality wise, the best results are acheived achieved using blind and Landweber. However these methods required a long processing time (several hours for big files) For more information about deconvolution method refer to paragraph 1.2.2 Choosing the deconvolution Method of the NIS _ Offline Deconvolution _ Manual .(pdf) |