Tabs Page |
---|
id | Description |
---|
title | Description |
---|
| Nikon Ti2-E fully motorized inverted microscopeRoger Gaudry Building, Room R-619 Instrument awarded to Dr Paradis-Bleau the Canadian Foundation for Innovation (CFI) Advanced Microscope Tier 1 usage price
Applications - Transmitted light, Bright-field
- Phase contrast
Polarized light, DIC Fluorescence - Live imaging
- Time-lapse imaging
Light sources Applications Brightfield Phase Contrast Polarized light Fluorescence
Light sources
Développer |
---|
title | Lumencor SpectraX complete specifications |
---|
|
Source | ID | Filter | nametype | Excitation Filter | Type | Working distance (mm) | Transmittance (% [nm]) |
---|
R | 640/30-25 | Plan Fluor | 2.1 | >75% [400-800] | G | 470/24-25 Excitation Filter | Dichroic mirror | Emission Filter | B | | 350/50x [325-375] | 400LP | 460/50m [435-485] | NT | 510/25-25 | Plan Apo Lambda | 0.13 | V | 395/25-25 | GY | 550\15-25 | Plan Apo Lambda | TBD | Storage GY | 575\25-25 | wavelengths (nm) | Compatible fluorophores | Nominal power (mW) |
---|
Violet | 395/25 | Bandpass | [382-407] | DAPI, Hoechst | 295 | Blue | | Bandpass | [430-450] | CFP | 256 | Cyan | 470/24 | Bandpass | [458-482] | FITC, GFP | 196 | Teal | 510/25 | Bandpass | [497-522] | YFP | 62 | Green/Yellow | 550/15 | Bandpass | [542-557] | TRITC, Cy3 | 260 | Green/Yellow (Storage) | 575/25 | Bandpass | [562-587] | mCherry | 310 | Red | 640/30 | Bandpass | [625-655] | Cy5 | 231 |
|
Objectives - 20x/0.5 Air Ph1 WD 2.1
Objectives 20x/0.5 Air Ph1- 60x/1.4 Oil DIC WD 0.13
- 100x/1.45 Oil Ph3 WD 0.13
- 100x/1.45 Oil DIC WD 0.13
Empty- 4x/0.2 Air WD 20
- 20x
\- /0.75 Air
DIC - DIC WD 1.0
Développer |
---|
title | Objectives complete specifications |
---|
|
Position | Name | Brand | IDFull name | Magnification | Numerical Aperture | Immersion | Type | Identifier | Working Working distance (mm) | Transmittance (% [nm]) | TechniqueTechniques | Cover glass thickness (mm) |
---|
1 | 20x/0.5 Air Ph1 | Nikon | 20x/0.5 Air Plan Fluor | WD 2.1 Ph1 | ? Air | MRH10201 | 20x | 0.5 | Air | Plan Fluor | 2.1 | >75% >80% [400- | 800750] | BF, Pol, PhC, Fluo | 0.17 | 2 | 60x/1.4 Oil DIC | Nikon | 60x/1.4 Oil Plan Apo Lambda DIC N2 | 60x | 1.4 | Plan Apo LambdaMRD01605 | 0.13 | >80% [ | 400475- | 750725] | BF, Pol, DIC, Fluo | 0.17 | 3 | | Nikon | 100x/1.45 Oil Plan Apo Lambda Ph3100x | MRD31905 | 10.45 | PPlan Apo Lambda | 0.13 | >75% [400-80013 | >80% [475-750] | BF, Pol, | DIC PhC, Fluo | 0.17 | 4 | 100x/1.45 Oil DIC | Nikon | 100x/1.45 Oil Plan Apo Lambda DIC N2 | 100x | 1.45 | MRD01905 | Plan Apo Lambda | 0.13 | >80% [475-750] | BF, Pol, DIC, Fluo | 0.17 | 5 | Empty | 6 | 20x4x/0.75 2 Air DIC | Nikon | 20x4x/0. | 75 2 Air Plan Apo Lambda | MRD00045 | 20 | >80% [400-1000] | BF, Fluo | 0.17 | 6 | 20x/0.75 Air DIC | Nikon | 20x/0.75 Air Plan Apo Lambda DIC N2 | MRD00205 | 1.0 | >80% [400-950] | DIC N220x | 0.75 | Air | Plan Apo Lambda | TBD | BF, Pol, DIC, Fluo | 0.17 |
|
FiltersFilter cubes DAPI /HoechstGFP/ AMCAFITC/EGFP TRITC/Rhodamine TexasRed
Développer |
---|
| title | Filters complete specifications |
---|
| Position | Cube name | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter |
---|
1 | DAPI/Hoechst/AMCA | Chroma | Cube set 31000v2 | 350/50x [325-375] | 400LP | 460/50m [435-485] | 2 | FITC/EGFP | Chroma | Cube set 41001 | 480/40x [420-500] | 505LP | 535/50m [510-560] | 3 | TRITC/Rhodamine | Chroma | Cube set 41002c | 545/30x [530-560] | 570LP | 620/60m [590-650] | 4 | Texas Red | Chroma | Cube set 41004 | 560/55x [532-588] | 595LP | 645/75m [607-682] | 5 | Empty | 6 | Empty | FITC (CFP) Cy5 DAPI/GFP/Cy3/Cy5 (requires Cy3 filter in Lumencor SpectraX Green/Yellow position) - DIC Analyzer
CFP/YFP/mCherry (requires mCherry filter in Lumencor SpectraX Green/Yellow position)
Développer |
---|
title | Filters complete specifications |
---|
|
Position | Name | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter | Comments |
---|
1 | DAPI | Nikon | DAPI-U HQ | 395/25x [383-408] | 425LP | 460/50m [435-485] | C-FL-C DAPI-U HQ | 2 | GFP | Semrock | GFP-4050B-000 | 466/40x [446-486] | 495LP | 525/50m
[500-550] | Nikon ID 96372 | 3 | Cy5 | Semrock | Cy5-5070A | 617/55x [590-645] | 652LP | 697/77m [659-736] | Nikon ID 96376 | 4 | DAPI/GFP/Cy3/Cy5 | Semrock | C182279 | None | 409/493/573/652 | 432/515/595/681 | 77074160 Custom Quad C182279. Excitation filters are in the Lumencor SpectraX light source | 5 | DIC Analyzer | Nikon | Ti2-C-DICACL | Not Applicable | Not Applicable | Not Applicable | Used for DIC imaging | 6 | CFP\YFP\mCherry | Semrock | C1997767 | None | 459/526/596 | 475/543/702 | Excitation filters are in the Lumencor SpectraX light source |
|
Tabs Page |
---|
id | User Guide |
---|
title | User Guide |
---|
|
UI Expand |
---|
| - Turn on the computer (#1)
- Remove the cover from the microscope
- Turn on the microscope power bar (#2)
If incubation is required, turn on the Okolab incubation module (#3A), the Lauda water bath (#3B) and open the CO2 (#3C) and N2 (#3D) tanks Avertissement |
---|
Make sure the humidifier and the water bath are clean and properly filled with distilled water. |
- Use your UdeM credentials to log in to Windows
- Start the software NIS-Elements
Info |
---|
The first time you use the instrument, you need to import the microscope settings into the software. To do this follow the instructions First use protocol. |
|
UI Expand |
---|
| - Save your data
- Close NIS-Elements software
- Transfer your data to the D: drive (Data Storage) or
|
| Tabs Page |
---|
id | User Guide |
---|
title | User Guide |
---|
| UI Expand |
---|
| Turn on the computerTurn on the microscope power barIf fluorescence is required, turn on the mercury lamp power supply unit (3A) and press ignition (3B) Avertissement |
---|
Mercury lamp must be on for at least 30 min before being turned off and vice-versa | Log in Windows using your UdM credentialsStart-up NIS-Elements Info |
---|
The first time you log in the computer, you need to import the microscope settings. To do this follow the instructions Setting-up NIS-Elements |
UI Expand |
---|
| - Save your data
- Close NIS-Elements
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
- Get your samples from the microscope
- If used, clean the oil objective with lens cleaner and lens paper
- If fluorescence was used, turn off the mercury lamp power supply unit (3A)
- Turn off the microscope power bar (2)
- Wait until the lamps are cool and cover the microscope
Remarque |
---|
| - Take back your samples including ones in the microscope
- Leave the microscope and the working area clean
- Mercury lamp must be on for at least 30 min before being turned off and vice-versa
|
UI Expand |
---|
| Files can be saved temporarily (during acquisition) on the local C: drive (desktop)At the end of each session, copy your data - to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname). Remarque |
---|
In any case, your files should be removed from the C: drive. |
| UI Expand |
---|
title | Setting-up NIS-Elements |
---|
| Ancre |
---|
Setting-up NIS-Elements | Setting-up NIS-Elements | This process is required the first time you are using the instrument. You will usually do it during the training session. It can also be performed if something is not working properly right or if you want to refresh the software interface. Remarque |
---|
This process will delete all experiment protocols and restore the parameters for the microscope. |
- Close NIS-Elements
- Wait until the complete closure of NIS-Elements
- Open the folder Desktop\Logiciels
- Open the software NIS Settings Utility
- Click on the Import tab
- Click on Browse
- Navigate to your desktop
- Select the file Nikon-E600 Settings.bin
- Click Select
- Select all items
- Click Import
- Click OK
- Close the NIS Settings
- Open NIS-Elements
|
Tabs Page |
---|
id | Lightpath |
---|
title | Light path |
---|
| Light pathDownload the schematics to follow Transmitted light (Bright field, Phase Contrast, Polarized light) and Reflected light (fluorescence) paths. Light path schematics.pdf |
Tabs Page |
---|
|