20x75 DIC N2

Tabs Container

id	Instrument Tabs
title	Nikon Eclipse E600Ti2-E
direction	horizontal

>75% 400800 DIC

Tabs Page

id	Description
title	Description

Nikon Ti2-E

fully motorized

inverted microscope

Roger Gaudry Building, Room R-619
Instrument awarded to Dr Paradis-Bleau the Canadian Foundation for Innovation (CFI)
Advanced Microscope Tier 1 usage price

Applications
- Transmitted light, Bright-field
- Phase contrast
- Polarized light, DIC
- Fluorescence
- Live imaging
- Time-lapse imaging
Applications
- Brightfield
- Phase Contrast
- Polarized light
- Fluorescence
Light sources
- LED lamp for transmitted light
- Lumencor SpectraX laser fluorescence for fluorescence

Développer

title	Lumencor SpectraX complete specifications

name

470/24-25

Source	ID	Filter	type	Excitation Filter	Type	Working distance (mm)	Transmittance (% [nm])
R	640/30-25	Plan Fluor	2.1	>75% [400-800]
G	Excitation Filter	Dichroic mirror	Emission Filter
B	440/20-25	350/50x [325-375]	400LP	460/50m [435-485]
NT	510/25-25	Plan Apo Lambda	0.13	V	395/25-25	GY	550\15-25	Plan Apo Lambda	TBD	Storage GY	575\25-25
wavelengths (nm)	Compatible fluorophores	Nominal power (mW)
Violet	395/25	Bandpass	[382-407]	DAPI, Hoechst	295
Blue	440/20	Bandpass	[430-450]	CFP	256
Cyan	470/24	Bandpass	[458-482]	FITC, GFP	196
Teal	510/25	Bandpass	[497-522]	YFP	62
Green/Yellow	550/15	Bandpass	[542-557]	TRITC, Cy3	260
Green/Yellow (Storage)	575/25	Bandpass	[562-587]	mCherry	310
Red	640/30	Bandpass	[625-655]	Cy5	231

Objectives
1. 20x/0.5 Air Ph1 WD 2.1
Objectives
20x/0.5 Air Ph1
2. 60x/1.4 Oil DIC WD 0.13
3. 100x/1.45 Oil Ph3 WD 0.13
4. 100x/1.45 Oil DIC WD 0.13
Empty
1. 4x/0.2 Air WD 20
2. 20x
\
1. /0.75 Air
DIC
1. DIC WD 1.0

400750

Développer

title	Objectives complete specifications

Technique WD 2.1 ? Air>75% 800

Position	Name	Brand	ID	Magnification	Numerical Aperture	Immersion	Type	Full name	Identifier	Working distance (mm)	Transmittance (% [nm])	Techniques	Cover glass thickness (mm)
1	20x/0.5 Air Ph1	Nikon	20x/0.5 Air Plan Fluor	Ph1		20x	0.5	Air	Plan Fluor	MRH10201	2.1	>80% [400-	750]	BF, Pol, PhC, Fluo	0.17
2	60x/1.4 Oil DIC	Nikon	60x/1.4 Oil Plan Apo Lambda DIC N2	60x	1.4

Remarque
Oil

MRD01605

Plan Apo Lambda

0.13

>80% [

475-

725]

BF, Pol, DIC, Fluo

0.17

3

100x/1.45 Oil Ph3

Nikon

100x/1.45 Oil Plan Apo Lambda Ph3

100x

1.45

Remarque
Oil

PPlan Apo Lambda

MRD31905

0.13

>80% [

475-

750]

BF, Pol,

PhC, Fluo

0.17

4

100x/1.45 Oil DIC

Nikon

100x/1.45 Oil Plan Apo Lambda DIC N2

100x

1.45

Remarque
Oil

MRD01905

Plan Apo Lambda

0.13

>80% [475-750]

BF, Pol, DIC, Fluo

0.17

5

Empty

6

20x4x/0.75 2 Air DIC

Nikon

4x/0.

2 Air Plan Apo Lambda

20x

MRD00045

20

>80% [400-1000]

BF, Fluo

0.17

6

20x/0.75 Air DIC

Nikon

20x/0.75 Air Plan Apo Lambda DIC N2

MRD00205

1.0

>80% [400-950]

0.75

Air

Plan Apo Lambda

TBD

BF, Pol, DIC, Fluo

0.17

FiltersFilter cubes
1. DAPI
/Hoechst/AMCA
FITC/EGFP
TRITC/Rhodamine

TexasRed

GFP/FITC (CFP)
Cy5
DAPI/GFP/Cy3/Cy5 (requires Cy3 filter in Lumencor SpectraX Green/Yellow position)
DIC Analyzer
CFP/YFP/mCherry (requires mCherry filter in Lumencor SpectraX Green/Yellow position)

400LP

Développer

title	Filters complete specifications

Position

Cube name

Name	Brand	ID	Excitation Filter	Dichroic mirror	Emission Filter	Comments
1	DAPI

/Hoechst/AMCA

Chroma

Cube set 31000v2

350/50x [325-375]

Nikon

DAPI-U HQ

395/25x
[383-408]

425LP

460/50m

[435-485]	C-FL-C DAPI-U HQ
2

FITC/EGFP

GFP

ChromaCube set 41001

480/40x [420-500]

505LP

535/50m [510-560]3TRITC/RhodamineChroma

Cube set 41002c

545/30x [530-560]570LP 620/60m [590-650]4Texas RedChroma

Cube set 41004

560/55x [532-588]

595LP 645/75m [607-682]5Empty6Empty

Detector
- Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6fps at full frame

Semrock	GFP-4050B-000	466/40x [446-486]	495LP	525/50m [500-550]	Nikon ID 96372
3	Cy5	Semrock	Cy5-5070A	617/55x [590-645]	652LP	697/77m [659-736]	Nikon ID 96376
4	DAPI/GFP/Cy3/Cy5	Semrock	C182279	None	409/493/573/652	432/515/595/681	77074160 Custom Quad C182279. Excitation filters are in the Lumencor SpectraX light source
5	DIC Analyzer	Nikon	Ti2-C-DICACL	Not Applicable	Not Applicable	Not Applicable	Used for DIC imaging
6	CFP\YFP\mCherry	Semrock	C1997767	None	459/526/596	475/543/702	Excitation filters are in the Lumencor SpectraX light source

Detector
- Hamamatsu ORCA Flash V2 C11440-22CU CMOS Monochrome Camera 2048 x 2048 pixels, 16-bit, 30 images/s at full frame

Save your data
Close NIS-Elements
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
Get your samples from the microscope
If used, clean the oil objective with lens cleaner and lens paper
If fluorescence was used, turn off the mercury lamp power supply unit (3A)
Turn off the microscope power bar (2)
Wait until the lamps are cool and cover the microscope

Tabs Page

id	User Guide
title	User Guide

UI Expand

expanded	true
title	Startup

Turn on the computer (#1)
Remove the cover from the microscope
Turn on the microscope power bar (#2)
If incubation is required, turn on the Okolab incubation module (#3A), the Lauda water bath (#3B) and open the CO₂ (#3C) and N₂ (#3D) tanks
Avertissement
Make sure the humidifier and the water bath are clean and properly filled with distilled water.
Use your UdeM credentials to log in to Windows
Start the software NIS-Elements

Info
The first time you use the instrument, you need to import the microscope settings into the software. To do this follow the instructions First use protocol.

UI Expand

title	Shutdown

Save your data
Close NIS-Elements software
Transfer your data to the D: drive (Data Storage) or

Tabs Page

id	User Guide
title	User Guide

Turn on the computer

Turn on the microscope power bar

If fluorescence is required, turn on the mercury lamp power supply unit (3A) and press ignition (3B)

UI Expand

expanded	true
title	Startup

Avertissement
Mercury lamp must be on for at least 30 min before being turned off and vice-versa

Log in Windows using your UdM credentials

Start-up NIS-Elements

Info
The first time you log in the computer, you need to import the microscope settings. To do this follow the instructions Setting-up NIS-Elements

UI Expand

title	Shutdown

Remarque

title	Important Reminders

Take back your samples including ones in the microscope
Leave the microscope and the working area clean
Mercury lamp must be on for at least 30 min before being turned off and vice-versa

UI Expand

title	Storage Management

Files can be saved temporarily (during acquisition) on the local C: drive (desktop)

At the end of each session, copy your data

to your external drive and delete it from the local C: drive

You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

Remarque
In any case, your files should be removed from the C: drive.

UI Expand

title	Setting-up NIS-Elements

AncreSetting-up NIS-ElementsSetting-up NIS-Elements

This process is required the first time you are using the instrument. You will usually do it during the training session. It can also be performed if something is not working properly right or if you want to refresh the software interface.

Remarque
This process will delete all experiment protocols and restore the parameters for the microscope.

Close NIS-Elements
Wait until the complete closure of NIS-Elements
Open the folder Desktop\Logiciels
Open the software NIS Settings Utility
Click on the Import tab
Click on Browse
Navigate to your desktop
Select the file Nikon-E600 Settings.bin
Click Select
Select all items
Click Import
Click OK
Close the NIS Settings
Open NIS-Elements

Tabs Page

id	Lightpath
title	Light path

Light path

Download the schematics to follow Transmitted light (Bright field, Phase Contrast, Polarized light) and Reflected light (fluorescence) paths.

Light path schematics.pdf

Tabs Page

id	Manuals
title	Manuals

Manuals

Tabs Page

id	Log
title	Log

UI Expand

expanded	true
title	To do

Adjust focus drive jitter
Add a holder for polarized light analyzer

UI Expand

title	2018-04-04

Nikon Preventive Maintenance
Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
100x slightly damaged but not the lens
Fluorescence cubes damaged: FITC TexasRed
Focus drive has a jitter

UI Expand

title	2021-07-20

Replacement mercury lamp bulb HBO 1003W/2
256 GB SSD added in workstation for OS
Windows 10 Installation
BIOS updated to v2.47
Camera Firmware updated to v2.11
NIS-Elements Basic Research v4.6 64-bits installed
Creation NIS Elements Settings
FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK

Tabs Page

id	Technical Datasheet
title	Technical Datasheet

Stand

Nikon Eclipse E600 upright Serial 725540

Light sources

Transmitted light Halogen 12V 100W Serial 01875599
Reflected light
- Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
- Bulb housing model LH M100C-1 Serial 039326

Condenser

Condenser Universal C-CU Serial 081901
Lens Dry NA 0.9
Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty

Objectives

10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
Empty
Empty
Empty

Développer

title	Objectives complete specifications

PositionNameBrandIDMagnificationNumerical ApertureImmersionTypeWorking distance (mm)Transmittance
(% [nm])TechniqueCover glass thickness (mm)110x/0.3 AirNikon10x/0.3 Air Plan Fluor Ph1 DLL10x0.3AirPlan Fluor16>75% [400-800]BF, Pol, PhC, Fluo0.172

40x/0.75 Air

Nikon40x/0.75 Air Plan Fluor Ph2 DLL40x

0.75

Air

Plan Fluor0.72>80% [400-750]BF, Pol, PhC, Fluo0.173

100x/1.3 Oil

Nikon100x/1.3 Oil Plan Fluor Ph3 DLL

100x

1.3

Remarque
Oil

Plan Fluor0.2>75% [400-800]BF, Pol, PhC, Fluo0.174Empty5Empty6Empty

Filters

DAPI/Hoechst/AMCA
FITC/EGFP
TRITC/Rhodamine
TexasRed

Développer

title	Filters complete specifications

PositionCube nameBrandIDExcitation FilterDichroic mirrorEmission Filter1DAPI/Hoechst/AMCAChromaCube set 31000v2 350/50x [325-375]400LP 460/50m [435-485]2FITC/EGFPChromaCube set 41001

480/40x [420-500]

505LP

535/50m [510-560]3TRITC/RhodamineChroma

Cube set 41002c

545/30x [530-560]570LP 620/60m [590-650]4Texas RedChroma

Cube set 41004

560/55x [532-588]

595LP 645/75m [607-682]5Empty6Empty

Detector

Color Camera Nikon DS-Ri2 Serial 701234

Workstation

HP Z440 Workstation
Intel Xeon E5-1630 v3 @ 3.7GHz
RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
OS 256GB SSD 550 MBs
2TB HD Data Storage 150 MBs
Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
Monitor HP Z24i display 24' 1920x1200

Consumables

Mercury lamp bulb OSRAM HBO 1003W/2 100W
Tungsten Halogen Lamp OSRAM 64623 HLX 100W 12V

Tabs Page

id	FAQ
title	Troubleshooting & FAQ

UI Expand

expanded	true
title	Troubleshooting

NIS-Elements shows an error: Camera Driver...

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

Turn off NIS-Elements
Turn on the camera (the microscope power bar and the camera switch on the top of the camera)
Check the USB conexion between the camera and the computer
Turn on NIS-Elements

UI Expand

title	FAQ

Can I use this microscope to look at cell in a dish?

No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.

Tabs Container

id	Demo Image
direction	horizontal

Tabs Page

id	Demo Images
title	Demo Images

Image Removed

Turn off the computer
If oil objectives were used, clean it with lens cleaner and lens paper (not Kimwipes)
If incubation was used, turn off the Okolab incubation module (#3A),Lauda water bath (#3B) and close the CO₂ (#3C) and N₂ (#3D) tanks
Wait until the SpectaX has cooled down and turn off the microscope power bar (#2)
Cover the microscope

Remarque

title	Important Reminders

Collect your samples, especially those in the microscope
Leave the microscope and workspace clean

UI Expand

title	Storage management

Files can be saved temporarily (during acquisition) to local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create one folder per laboratory using the principal investigator's last name. Inside, create a folder per user using the following nomenclature (First Name_Last Name).

Remarque

title	Important

In any case, do not store your files on the C: drive.

UI Expand

title	First use

Ancre

	FirstUse
	FirstUse

When using the microscope for the first time, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

Remarque
This process will delete all experiment protocols and reset the software to the original settings for this specific microscope.

If open, close NIS-Elements and wait until it is completely closed (up to 30 seconds)
On your Desktop open the Softwares folder
Open NIS Settings Utility
Click on the Import tab
Click on Browse
Navigate to your Desktop
Select the file Nikon-Ti2 Settings.bin
Click Select
Select all items
Click Import
Click OK
Close the NIS Settings
You can now reopen NIS-Elements

Tabs Page

id	Light path
title	Light path

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.

PDF

name	LightPath_Nikon Ti2.pdf

Tabs Page

id	Manuals
title	Manuals

Available manuals

Tabs Page

id	Log
title	Log

UI Expand

expanded	true
title	To do

Check condenser filters if labels match
Check why 20x/0.75 DIC objective has no DIC prism
Condenser DIC prism is DIC N1 while objective requires N2
Check why at 4x Condenser gives a black shadow on the righ side of the image
Adjust Objective XYZ offset
Check plane because FOV is not flat

UI Expand

title	2024-03-20 Incubation maintenance

Fixed incubator tubing misplaced
Added better attachment for tubing on the incubation chamber
Checked incubation chamber for obstruction

UI Expand

expanded	true
title	2023-11-29

Fixed condenser holder loose
20x/0.5 Ph1 dirty or damaged likely dirty with varnished

UI Expand

title	2022-09-22

Added network cable between Acquisition and Processing workstations
Added shortcuts in Desktop\Documentation

UI Expand

title	2022-03-16

Clean 60x objective (nail polish on it !)
Fix damaged stage insert
Stage insert tilt adjustment
Objective XY offset adjustments

UI Expand

title	2022-03-16

Installed new objective 4x/0.2 Plan Apo Lambda
Updated configuration file

UI Expand

title	2022-02-21

Computer maintenance
Imaging test slides
Re-install DCAM API drivers v22.2.6391
Okolab incubation complete maintenance
- Disassemble Okolab stage insert
- Disconnect the pipes
- Disconnect the Lauda water-bath
- Clean everything with 10% acetic acid (excepted the plastic pipes and washers)
- Rinse with regular tap water 2 times
- Rinse with distilled water once
- Dry, reassemble and reconnect
- Refill with the Lauda water-bath with 3L of milliQ water
- Set the Okolab temperature Control Mode to Chamber
- Set the Okolab temperature to 65°C
- Let run for 1h. This will sterilize the water.
- Check for any leakage
- Set the Okolab temperature Control Mode back to Sample
- Set the Okolab temperature back to 37°C

UI Expand

title	2021-12-16

Cleaning objectives
Control for incubation chamber liquid intrusion

UI Expand

title	2021-11-30

CO₂ Calibration for Okolab: Offset of 1%

UI Expand

title	2021-11-05

Control for illumination quality
See Illumination Report_Nikon Ti2_2021-11-05.pdf
Liquid light guide adjustment

UI Expand

title	2021-09-16

20x/0.75 DIC objective was dirty and has been cleaned
Printed and displayed a Memo about available air and oil objectives
Adjustment of camera angle
Objective calibration
Objective XY offset
Updated NIS settings
Added light path schematics to wikipedia

UI Expand

title	2021-09-15

Okolab display a NAN message for the free thermal sensor
The probe is made of two wires that need to be in contact to measure the temperature properly
- Turn off the Okolab module
- Disconnect the free sensor probe
- Cut out the damaged part
- Carefully expose the two wires
- Connect the two wires together

UI Expand

title	2021-09-13

Added label on gas bottles
Added line mark on humidifier

UI Expand

title	2021-07-20

512 GB SSD installed for OS
Windows 10 Installation
BIOS updated to v2.47

Tabs Page

id	Technical Datasheet
title	Technical Datasheet

Stand

Nikon Ti2-E inverted Serial 540156 System 170110-Sys-006287

Light sources

Transmitted LED light
- ND32 filter
- IR filter
- Manual Polarizer
Lumencor SpectraX 6-NII-SE Serial 9409

Condenser

Motorized condenser Ti2-C-TC-E Serial 519097
Lens LWD NA 0.52
Filter turret 7 motorized positions
1. Empty
2. Empty
3. Ph1
4. Ph3
5. Shutter
6. Empty
7. DIC N1

Objectives

20x/0.5 Air Ph1 WD 2.1
60x/1.4 Oil DIC WD 0.13
100x/1.45 Oil Ph3 WD 0.13
100x/1.45 Oil DIC WD 0.13
4x/0.2 Air WD 20
20x/0.75 Air DIC WD 1.0

Stage

Motorized stage Ti2 SHU compatible Serial 127808
Remote control joystick Ti2-S-JS Serial 127976
Inserts
- Multi-well plate Ti2-S-HW with tilt adjustment (no incubation)
- Combo slide 3cm dish Ti2-S-HU with tilt adjustment (no incubation)
- Okolab H101-CellASIC Frame with perfusion ports
  - 1 x 35mm petri dish 1x35-M + cover
  - 2 Chamber Slide 2xGS-M+ cover
  - 1 Multi-well for oil objectives MW-OIL
  - 6-well plate 6MW+ cover
  - 1 CellASIC + cover

Filters

DAPI Cube Ex 383-408 DAPI-U DM 425 BA 435-485
GFP Cube Semrock 96372 M349727 17
Cy5 Cube Semrock 96376 M351081 8
77074160 Custom Quad C182279 Polychroic and quad bandpass emitter for use with the following single bandpass filters: ET395/25x, ET470/24x, ET550/15x, ET640/30x
DIC Analyser Ti2-C-DICACL
C197767 7707\4656 CFP\YFP\mCherry XT

Detector

Hamamatsu ORCA Flash V2 C11440-22CU CMOS Monochrome Camera 2048 x 2048 pixels, 16-bit, 30fps at full resolution Serial 101081

Workstation

HP Z440 Workstation
Intel Xeon E5-1620 v4 @ 3.5GHz
RAM 32 GB DDR4 2400 MHz ECC (4 x 8 GB)
OS 500 GB SSD 530 MB/s
4 TB HD Data Storage (2 x 2 TB spanned volume) 130 MB/s
Video Card nVidia GTX 1080 8GB DDR5 dedicated memory
Monitor HP Z24i display 24' 1920x1200
Software NIS-Elements AR v5.02

Incubation

Okolab BoldLine Temperature unit Serial 284-1058 H101 T Unit BL
Okolab BoldLine CO₂/O₂ Unit 0-10/1-18 Serial 088-1102
Okolab OkoTouch Serial 118-224
Lauda water-bath Model Eco RE415 S LCK 4910 Serial LCK-4910-16-0006 Okolab 1322-1006 2017-05-30

Consumables

CO₂ Tank
N₂ Tank
Liquid Light Guide

Tabs Page

id	FAQ
title	Troubleshooting & FAQ

Troubleshooting

UI Expand

title	Chamber does not reach the desired temperature (or is very slow to warm up)

This can happen when the liquid running through the chamber is not flowing properly.

Pause your experiment
Turn off the Okolab environment controller 3A and 3B
Disconnect the blue end of the connection pipe (at the junction with the spring shape objective warmer)
Place the open end into a dish to collect liquid
Turn the Lauda water bath back ON (3B)
The liquid should flow quite rapidly, if not proceed as follow
- Turn OFF the Lauda water bath (3B)
- Take the 20 mL syringe located inside the drawer labelled Tubing
- Connect it to the open end of the blue pipe
- Use the syringe to blow pressurized air into the pipes to clear it out
- Remove the syringe (and store it back into the Tubing drawer)
- Test if the liquid is now flowing properly by turning the Lauda water bath back ON (3B)
  - If not repeat the procedure
  - If so, turn OFF the Lauda water-bath (3B)
  - Reconnect the blue and green pipes together
  - Turn the Okolab environment controller 3A and 3B back ON

Avertissement
Be careful not to touch your sample when performing these actions as it may displace your current acquisition

Info

title	How the Okolab chamber works

The liquid from the water bath enters the lead first via the red pipe
Then it goes through the lead circuitry to keep it warm
It exists via the unlabeled tube and enters the incubation chamber main body
It goes through the circuitry of the main body and exits via the green labelled tube

One must maintain a decent amount of liquid in the Lauda water bath to avoid bubble formation in the circuitry

Because the circuitry inside the top lead and the incubation chamber main body is thin it can get clogged. The procedure above can solve this issue.

UI Expand

title	Liquid forming inside the incubation chamber

This happens when the mixed-gas humidifier is overfilled. Bubbles created by the gas going through the humidifier can bring liquid into the gas feed line.

Turn off the Okolab module and the water bath
Remove your sample and store it properly
Dry the incubation chamber with a clean tissue
Carefully remove the cap of the humidifier glass bottle

Avertissement
Pay extra care when manipulating the humidifier bottle as it is made of glass and is very fragile

Remove distilled water from the humidifier

Info
Humidifier shouldn't be more than 2/3^rd filled

Close the humidifier by replacing the cap
Turn on the Okolab module and the water bath

UI Expand

title	Liquid forming inside the incubation chamber but the humidifier is not overfilled

This can happen during long experiments. The high humidity of the gas mixture condensates in the pipe between the humidifier and the incubation chamber.

Pause your experiment
Disconnect both ends of the yellow tube from the humidifier
Drain the tube from any liquid
Reconnect the yellow tube to the humidifier
You can use a syringe or gas pressure to blow out any liquid from the incubation chamber cover

Avertissement
Be careful not to touch your sample when performing this action

Wipe any liquid with a tissue
Reconnect the yellow tube to the incubation chamber

FAQ

UI Expand

title	Can I use this microscope to look at cell in a dish?

Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
The objectives are optimized to image through thin glass bottom multi-well plates
You may also image specimen mounted between a slide and a 0.17mm thick coverslip
For long timelapse, be aware of photo-toxicity

UI Expand

title	How can I warm up the incubation chamber faster?

You may use a trick to warmup the incubation chamber faster. Using this method. you should reach a stable temperature within 30 minutes.

Avertissement
Do NOT proceed with your sample but with a blank control (dish with distilled water for example)

Place your blank control in the incubation chamber
Immerge the tip of the sample temperature probe in the blank
Set the Okolab temperature Control Mode to Chamber (Settings>Temperature>Control Mode>Chamber>Save)
Set the Okolab temperature to 50°C (Home>Temperature>50°C>Set)
Check the temperature of the sample (Menu Magnifier>Sample Temperature). It should take between 10 to 15 minutes for the blank to reach 30°C.

Info
The Lauda water bath is faster to warm up water than to cool it down. Make sure to anticipate and not pass beyond the desired temperature. If your desired temperature is 37°C, you can stop the procedure when the blank has reached 30°C.

When the blank has reach the desired temperature

Set the Okolab temperature back to 37°C (Home>Temperature>37°C>Set)
Set the Okolab temperature Control Mode to Sample (Settings>Temperature>Control Mode>Sample>Save)
Wait 10 to 20 minutes until the temperature stabilizes
Then you can safely replace the blank with your sample

Tabs Container

id	Demo Image
title	Nikon Ti2-E
direction	horizontal

Tabs Page

id	Demo Image
title	Demo Image

Image Added

Raccourcis espace

Arborescence des pages

Comparaison des versions

Ancienne version 3

Nouvelle version Actuel

Légende

Nikon Ti2-E

inverted microscope

Light path

Manuals

Stand

Light sources

Condenser

Objectives

Filters

Detector

Workstation

Consumables

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.

Stand

Light sources

Condenser

Objectives

Stage

Detector

Workstation

Incubation

Consumables

Troubleshooting

FAQ

Raccourcis espace

Arborescence des pages

Historique de la page

Comparaison des versions

Ancienne version 3

Nouvelle version Actuel

Légende

Nikon Ti2-E

inverted microscope

Light path

Manuals

Stand

Light sources

Condenser

Objectives

Filters

Detector

Workstation

Consumables

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.

Stand

Light sources

Condenser

Objectives

Stage

Detector

Workstation

Incubation

Consumables

Troubleshooting

FAQ