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id | Description |
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title | Description |
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| Nikon Ti2-E fully motorized inverted microscopeRoger Gaudry Building, Room R-619 Instrument awarded to Dr Paradis-Bleau the Canadian Foundation for Innovation (CFI) Advanced Microscope Tier 1 usage price
Applications - BrightfieldTransmitted light, Bright-field
- Phase contrast
Polarized light, DIC Fluorescence - Live imaging
- Long timelapse Time-lapse imaging
Light sources
Développer |
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title | Lumencor SpectraX complete specifications |
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Light sourceSource | Filter ID | Filter type | Excitation wavelengths (nm) | Compatible fluorophores | Power Nominal power (mW) |
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Violet | 395/25 | Bandpass | [382-407] | DAPI, Hoechst | 295 | Blue | | Bandpass | [430-450] | CFP | 256 | Cyan | 470/24 | Bandpass | [458-482] | FITC, GFP | 196 | Teal | 510/25 | Bandpass | [497-522] | YFP | 62 | Green/Yellow | 550/15 | Bandpass | [542-557] | TRITC, Cy3 | 260 | Green/Yellow (Storage) | 575/25 | Bandpass | [562-587] | mCherry | 310 | Red | 640/30 | Bandpass | [625-655] | Cy5 | 231 |
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Objectives - 20x/0.5 Air Ph1 WD 2.1
- 60x/1.4 Oil DIC WD 0.13
- 100x/1.45 Oil Ph3 WD 0.13
- 100x/1.45 Oil DIC WD 0.13
- Empty
20x- 4x/0.2 Air WD 20
- 20x/0.75 Air
DIC - DIC WD 1.0
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title | Objectives complete specifications |
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Position | Name | Brand | Full name | Product ID | Magnification | Numerical Aperture | Immersion | Type | Identifier | Working distance (mm) | Transmittance (% [nm]) | TechniqueTechniques | Cover glass thickness (mm) |
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1 | 20x/0.5 Air Ph1 | Nikon | 20x/0.5 Air Plan Fluor Ph1 | MRH10201 | 20x | 0.5 | Air | Plan Fluor | 2.1 | >80% [400-750] | BF, Pol, PhC, Fluo | 0.17 | 2 | 60x/1.4 Oil DIC | Nikon | 60x/1.4 Oil Plan Apo Lambda DIC N2 | MRD01605 | 60x | 1.4 | Plan Apo Lambda | 0.13 | >80% [475-725] | BF, Pol, DIC, Fluo | 0.17 | 3 | | Nikon | 100x100x/1.45 Oil Plan Apo Lambda Ph3 | MRD31905 | 100x | 1.45 | Plan Apo Lambda | 0.0.13 | >80% [475-750] | BF, Pol, PhC, Fluo | 0.17 | 4 | 100x/1.45 Oil DIC | Nikon | 100x/1.45 Oil Plan Apo Lambda DIC N2 | MRD01905 | 100x | 1.45 | Plan Apo Lambda | 0.13 | >80% [475-750] | BF, Pol, DIC, Fluo | 0.17 | 5 | Empty | 6 | 20x4x/0.75 2 Air DIC | Nikon | 20x4x/0. | 75 2 Air Plan Apo Lambda | DIC N2 | MRD00205 | 20x | 0.75 | Air | Plan Apo Lambda | 1.0 | MRD00045 | 20 | >80% [400-1000] | BF, Fluo | 0.17 | 6 | 20x/0.75 Air DIC | Nikon | 20x/0.75 Air Plan Apo Lambda DIC N2 | MRD00205 | 1.0 | >80% [400-950] | BF, Pol, DIC, Fluo | 0.17 |
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Filter cubesFilters DAPI GFP/FITC (CFP) Cy5 DAPI/GFP/Cy3/Cy5 (requires Cy3 filter in Lumencor SpectraX Green/Yellow position) - DIC Analyzer
- DAPI/
CFP/YFP/mCherry (requires mCherry filter in Lumencor SpectraX Green/Yellow position)
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title | Filters complete specifications |
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Position | Cube nameName | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter | Comments |
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1 | DAPI | Nikon | DAPI-U HQ | 395/25x 25x [383-408] | 425LP | 460/50m 50m [435-485] | C-FL-C DAPI-U HQ | 2 | GFP | Semrock | GFP-4050B-000 | 466/40x [446-486] | 495LP | 525/50m
[500-550] | Nikon ID 96372 | 3 | Cy5 | Semrock | Cy5-5070A | 617/55x 55x [590-645] | 652LP | 697/77m 77m [659-736] | Nikon ID 96376 | 4 | DAPI/GFP/Cy3/Cy5 | Semrock | C182279 | None | 409/493/573/652 | 432/515/595/681 | 77074160 Custom Quad C182279. Excitation filters are in the Lumencor SpectraX light source | 5 | DIC Analyzer | Nikon | Ti2-C-DICACL | Not Applicable | Not Applicable | Not Applicable | Used for DIC imaging | 6 | CFP\YFP\mCherry | Semrock | C1997767 | None | 459/526/596 | 475/543/702 | Excitation filters are in the Lumencor SpectraX light source |
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id | User Guide |
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title | User Guide |
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| - Turn on the computer (#1)
- Remove the cover from the microscope
- Turn on the microscope power bar (#2)
If incubation is required, turn on the Okolab incubation module (3A and 3B#3A), the Lauda water bath (#3B) and open the CO2 (3C#3C) and N2 (3D#3D) tanks Avertissement |
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Make sure the humidifier and the water bath are clean and properly filled with distilled water. |
- Log in Windows using your UdM credentials
- Use your UdeM credentials to log in to Windows
- Start the software Start-up NIS-Elements
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The first time you log in the computeruse the instrument, you need to import the microscope settings into the software. To do this follow the instructions Setting-up NIS-Elementsinstructions First use protocol. |
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| - Save your data
- Close NIS-Elements software
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
- Get your samples from the microscope
- Turn off the computer
- If oil objectives were used, clean it Clean the oil objectives with lens cleaner and lens paper (not Kimwipes)
- If incubation was used, turn off the Okolab incubation module (3A and 3B) and close #3A),Lauda water bath (#3B) and close the CO2 (3C#3C) and N2 (3D#3D) tanks
- Turn Wait until the SpectaX has cooled down and turn off the microscope power bar (2#2)
- Cover the microscope
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| - Take back Collect your samples including ones , especially those in the microscope
- Leave the microscope and the working area workspace clean
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title | Storage Managementmanagement |
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| - Files can be saved temporarily (during acquisition) on the to local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a one folder per laboratory using the principal investigator's last name. WithinInside, create one a folder per user (Firstname_Lastnameusing the following nomenclature (First Name_Last Name).
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| In any case, do not store your files should be removed from on the C: drive. |
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title | Setting-up NIS-ElementsFirst use |
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| Setting-up NIS-Elements | When using the microscope for Setting-up NIS-Elements | This process is required the first time, you are using the instrument. need to import the microscope settings into the software. You will usually do it this during the training session. It This procedure can also be performed if something is not working properly right or and if you want to refresh reset the software interface to the its original settings. Remarque |
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This process will delete all experiment protocols and restore the parameters for the reset the software to the original settings for this specific microscope. |
- Close If open, close NIS-Elements
- Wait until the complete closure of NIS-Elements
- Open the folder Desktop\Logiciels
- and wait until it is completely closed (up to 30 seconds)
- On your Desktop open the Softwares folder
- Open Open the software NIS Settings Utility
- Click on the Import tab
- Click on Browse
- Navigate to your desktop Desktop
- Select the file Nikon-Ti2 Settings.bin
- Click Select
- Select all items
- Click Import
- Click OK
- Close the NIS Settings
- Open You can now reopen NIS-Elements
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id | LightpathLight path |
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title | Light path |
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| The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.
Light pathDownload the schematics to follow Transmitted light (Bright field, Phase Contrast, Polarized light, DIC) and Reflected light (fluorescence) paths. LightPath |
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ManualsAvailable manuals |
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| - Add light path to wiki
- Check condenser filters if labels match
- Check why 20x/0.75 DIC objective has no DIC prism
- Check 20x DIC objective
- Condenser DIC prism is DIC N1 while objective requires N2
- Print a memo about objective DIC vs Ph and Oil vs Air
- Check why at 4x Condenser gives a black shadow on the righ side of the image
- Adjust Objective XYZ offset
- Check plane because FOV is not flatUpdate NIS Settings (remove unused layout, perform objective calibration)
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title | 20212024-0903-1520 Incubation maintenance |
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| - Fixed incubator tubing misplaced
- Added better attachment for tubing on the incubation chamber
- Checked incubation chamber for obstruction
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expanded | true |
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title | 2023-11-29 |
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| - Added network cable between Acquisition and Processing workstations
- Added shortcuts in Desktop\Documentation label on gas bottlesAdded line mark on humidifier
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| - 512 GB SSD installed for OS
- Windows 10 Installation
- BIOS updated to v2.47
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id | Technical Datasheet |
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title | Technical Datasheet |
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| Stand- Nikon Ti2-E inverted Serial 540156 System 170110-Sys-006287
Light sources- Transmitted LED light
- ND32 filter
- Manual Polarizer
- Lumencor SpectraX 6-NII-SE Serial 9409
CondenserObjectives- 20x/0.5 Air Ph1
- 60x/1.4 Oil DIC WD 0.13
- 100x/1.45 Oil Ph3 WD 0.13
- 100x/1.45 Oil DIC WD 0.13
- Empty
- 20x/0.75 Air DIC
Stage- Motorized stage Ti2 SHU compatible Serial 127808
- Remote control joystick Ti2-S-JS Serial 127976
- Inserts
- Multi-well plate Ti2-S-HW with tilt adjustment (no incubation)
- Combo slide 3cm dish Ti2-S-HU with tilt adjustment (no incubation)
- Okolab H101-TI2-S-HW Frame with perfusion ports
- 1 x 35mm petri dish 1x35-M
- 2 Chamber Slide 2xGS-M
- 1 Multi-well for oil objectives MW-OIL
- 6-well plate 6MW
- 1 CellASIC
Each Okolab insert comes with a bottom holder and a top cover
Filters - DAPI Cube Ex 383-408 DAPI-U DM 425 BA 435-485
- GFP Cube Semrock 96372 M349727 17
- Cy5 Cube Semrock 96376 M351081 8
- 77074160 Custom Quad C182279 Polychroic and quad bandpass emitter for use with the following single bandpass filters: ET395/25x, ET470/24x, ET550/15x, ET640/30x
- DIC Analyser Ti2-C-DICACL
- C197767 7707\4656 CFP\YFP\mCherry XT
Detector- Hamamatsu ORCA Flash V2 C11440-22CU CMOS Monochrome Camera 2048 x 2048 pixels, 16-bit, 30fps at full frame Serial 101081
Workstation- HP Z440 Workstation
- Intel Xeon E5-1620 v4 @ 3.5GHz
- RAM 32 GB DDR4 2400 MHz ECC (4 x 8 GB)
- OS 500GB SSD 550 MB/s
- 4TB HD Data Storage (2 x 2 TB spanned volume) 170 MB/s
- Video Card nVidia GTX 1080 8GB DDR5 dedicated memory
- Monitor HP Z24i display 24' 1920x1200
- Software NIS-Elements AR v5.02
Incubation- Okolab BoldLine Temperature unit Serial 284-1058 H101 T Unit BL
- Okolab BoldLine CO2/O2 Unit 0-10/1-18 Serial 088-1102
- Okolab OkoTouch Serial 118-224
Consumables |
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