| Tabs Page |
|---|
| id | Description |
|---|
| title | Description |
|---|
| Nikon Eclipse E-600 upright microscopeRoger Gaudry Building, Room N-620
Simple Microscope usage price
Applications - Brightfield
Bright-field Phase Contrast Polarized light Fluorescence - Color camera
Light sources
Objectives Objectives:10x/0.3 Air Ph1 WD 16.0 - 20x/0.5 Air DIC 2.1
40x/0.75 Air Ph2 WD 0.72 - 60x/0.85 Air WD 0.3
- 100x/1.3 Oil Ph3 WD 0.2
| Développer |
|---|
| title | Objectives complete specifications |
|---|
|
| Position | Name | Brand | Full name | Numerical Aperture | Immersion | Type | Working distance (mm) | Transmittance
(% [nm]) | Technique | Cover glass thickness (mm) |
|---|
| 1 | 4x/0.13 Air | Nikon | 4x/0.3 Air Plan Fluor | 0.13 | Air | Plan Fluor | 17.1 |
| BF, Fluo | - | | 2 | 10x/0.3 Air | Nikon | 10x/0.3 Air Plan Fluor Ph1 DLL | 0.3 | Air | Plan Fluor | 16 | >75% [400-800]
Max % @500nm | BF, Pol, PhC, Fluo | 0.17 | | 3 | | Nikon | 20x/0.5 Air Plan Fluor DIC M | 0.5 | | Plan Fluor | 2.1 |
| BF, Fluo | 0.17 | | 4 | 40x/0.75 Air | Nikon | 40x/0.75 Air Plan Fluor Ph2 DLL | 0.75 | Air | Plan Fluor | 0.72 | >80% [400-750]
Max % @500nm | BF, Pol, PhC, Fluo | 0.17 | | 5 | 60x/0.85 Air | Nikon | 60x/0.85 Air Plan Fluor | 0.85 | Air | Plan Fluor | 0.30 |
| BF, Fluo | Ajustable 0.11-0.23 | | 6 | | Nikon | 100x/1.3 Oil Plan Fluor Ph3 DLL | 1.3 | | Plan Fluor | 0.2 | >75% [400-800]
Max % @500nm
| BF, Pol, PhC, Fluo | 0.17 |
|
Filter cubes DAPI/Hoechst/AMCA FITC/EGFP TRITC/Rhodamine TexasRed - Empty
- Empty
| Développer |
|---|
| title | Filters complete specifications |
|---|
|
|
TxRed |
| Tabs Page |
|---|
| id | User Guide |
|---|
| title | User Guide |
|---|
|
| UI Expand |
|---|
| expanded | true |
|---|
| title | Start-up |
|---|
|
Startup computer- computer (#1)
- Turn on the microscope power
|
bar- bar (#2)
If fluorescence is required, press the mode button on the lumencor remote If transmitted light is required,
|
turn mercury lamp power supply unit (3A) and press ignition (3B)| Avertissement |
|---|
Mercury lamp must be on for at least 30 min before being turned off and vice-versa |
halogen lamp on the right side of the microscope (#3) Log in Windows using your UdM credentials - Start-up NIS-Elements
|
log in the computeruse the instrument, you need to import the microscope settings into the software. To do this follow the instructions |
|
Nikon Eclipse E600-en
| UI Expand |
|---|
| - Save your data
- Close NIS-Elements
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
|
Get your samples from the microscope- Turn off the computer
- If oil objective was used, clean
|
the oil objective - it with lens cleaner and lens paper
|
If fluorescence was used, turn off the mercury lamp power supply unit (3A- (not Kimwipes)
- Turn off the microscope power bar (
|
2Wait until the lamps are cool and cover - Cover the microscope
| Remarque |
|---|
| - Take back your samples including ones in the microscope
- Leave the microscope and the working area clean
|
|
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
Storage Management | - Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
| Remarque |
|---|
In any case, your files should be removed from the C: drive. |
|
| Ancre |
|---|
| Setting-up NIS-Elements | Setting-up NIS-Elements | Setting-up NIS-ElementsThis is required the first time you are using the instrument
| UI Expand |
|---|
| The first time you use the instrument, you need to import the microscope settings into the software. You will usually do |
it this during the training session. |
It
This procedure can also be performed if something is not working properly
|
right or refresh interfaceOpen the software to its original settings. | Remarque |
|---|
This process will delete all experiment protocols and |
restore the parameters for the microscope |
Close NIS-ElementsWait until the complete closure of NIS-ElementsOpen the folder Desktop\Logicielsreset the software to the original settings for this specific microscope. |
- If open, close NIS-Elements and wait until it is completely closed (up to 30 seconds)
- On your Desktop open the Softwares folder
- Open
|
- NIS Settings Utility
- Click on the Import tab
- Click on Browse
- Navigate to your
|
desktop- Desktop
- Select the file Nikon-E600 Settings.bin
- Click Select
- Select all items
- Click Import
- Click OK
- Close the NIS Settings
- You can now reopen NIS-Elements
|
| Tabs Page |
|---|
| id | Lightpath |
|---|
| title | Lightpath |
|---|
|
Follow the schematics below for Transmitted light (Bright The following schematics depict the light path for transmitted (bright-field, Phase Contrast, Polarized light) and transmitted light reflected (fluorescence) lights.view-file| PDF |
|---|
| name | LightPath_Nikon E600.pdf |
|---|
|
| height | 400
|---|
| Tabs Page |
|---|
|
Available manuals
|
| Tabs Page |
|---|
|
| UI Expand |
|---|
| - Adjust focus drive jitter
- Add a holder for polarized light analyzer
- Bring new test slides
|
| UI Expand |
|---|
| expanded | true |
|---|
| title | 2022-09-26 |
|---|
| - Added Lumencor SOLA v-nIR
- Added Fluorescent light collimator
|
| UI Expand |
|---|
| expanded | true |
|---|
| title | 2022-09-26 |
|---|
| - Added 4x/0.13 Air
- Added 20x/0.5 Air
- Added 60x/0.85 Air
- Updated E600 settings file
|
| UI Expand |
|---|
| - Replacement mercury lamp bulb HBO 1003W/2
- 256 GB SSD added in workstation for OS
- Windows 10 Installation
- BIOS updated to v2.47
- Camera Firmware updated to v2.11
- NIS-Elements Basic Research v4.6 64-bits installed
- Creation NIS Elements Settings
- FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK
|
| UI Expand |
|---|
| - Nikon Preventive Maintenance
- Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
- 100x slightly damaged but not the lens
- Fluorescence cubes damaged: FITC
|
TxRed- , TexasRed
- Focus drive has a
|
jutter
|
| Tabs Page |
|---|
| id | Technical Datasheet |
|---|
| title | Technical Datasheet |
|---|
| Stand- Nikon Eclipse E600 E600W upright Serial 725540
Light sources- Transmitted light Halogen 12V 100W Serial Model C-LPSerial 01875599
- Reflected light
Condenser- Condenser Universal C-CU Serial 081901
- Lens Dry NA 0.9
- Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty
Objectives- 4x/0.13
- 10x/0.3 Plan Fluor Ph1 DLL WD 16 .0 Air
- 20x/
- 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
- 60x/0.85
- 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil Oil with PF/PA 100 Oil Wollaston prismEmpty
StageEmptyEmptyFiltersDAPI/Hoechst/AMCA FITC/EGFP TRITC/Rhodamine TexasRed
DetectorTurret - DAPI 31000 C50615
- FITC HQ 41001 C54729
- RFP HQ 41002C C54730
- Texas Red HQ 41004 C54731
- Empty
- Empty
Detector - Camera Nikon DS-Ri2 Serial 701234
Workstation- HP Z440 Workstation
- Intel Xeon E5-1630 v3 @ 3.7GHz
- RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
- OS 256GB 256 GB SSD 550 MBs
- 2TB 2 TB HD Data Storage 150 MBs
- Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
- Monitor HP Z24i display 24' 1920x12001920 x 1200
ConsumablesMercury lamp bulb OSRAM HBO 1003W/2 100W |
| Tabs Page |
|---|
| id | FAQ |
|---|
| title | Troubleshooting & FAQ |
|---|
| Troubleshooting| UI Expand |
|---|
| title | NIS-Elements shows an error: Camera Driver... |
|---|
| This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on. - Turn off NIS-Elements
- Turn on the camera (both the microscope power bar and the camera need to be ON
(the camera is usually ON, the switch on the top of the camera) - Check the camera is correctly connected to the computer via a USB cable
- Turn on NIS-Elements
|
FAQ| UI Expand |
|---|
| title | Can I use this microscope to look at cells in a dish? |
|---|
| No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip. |
|
|
![>75% [400-800]](https://wiki.umontreal.ca/download/attachments/189565708/10x-0.3%20Plan%20Fluor%20DLL.png?version=1&modificationDate=1627524548000&api=v2){kind=link}
![>80% [400-750]](https://wiki.umontreal.ca/download/attachments/189565708/40x-0.75%20Plan%20Fluor%20Ph2%20DLL.png?version=1&modificationDate=1627524687000&api=v2){kind=link}
![>75% [400-800]](https://wiki.umontreal.ca/download/attachments/189565708/100x-1.3%20Plan%20Fluor%20Ph3%20DLL.png?version=1&modificationDate=1627524762000&api=v2){kind=link}