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id | Description |
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title | Description |
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| Nikon Ti2-E fully motorized inverted microscopeRoger Gaudry Building, Room R-619 Instrument awarded to Dr Paradis-Bleau the Canadian Foundation for Innovation (CFI) Advanced Microscope Tier 1 usage price
Applications - Transmitted light, Bright-field
- Phase contrast
Applications - Brightfield
Polarized light, DIC Fluorescence - Live imaging
- Long timelapse Time-lapse imaging
Light sources
Développer |
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title | Lumencor SpectraX complete specifications |
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Source | ID | Filter | nametype | Excitation Filter | Type | Working distance (mm) | Transmittance (% [nm]) |
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R | 640/30-25 | Plan Fluor | 2.1 | >75% [400-800] | G | 470/24-25 Excitation Filter | Dichroic mirror | Emission Filter | B | | 350/50x [325-375] | 400LP | 460/50m [435-485] | NT | 510/25-25 | Plan Apo Lambda | 0.13 | V | 395/25-25 | GY | 550\15-25 | Plan Apo Lambda | TBD | Storage GY | 575\25-25 | wavelengths (nm) | Compatible fluorophores | Nominal power (mW) |
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Violet | 395/25 | Bandpass | [382-407] | DAPI, Hoechst | 295 | Blue | | Bandpass | [430-450] | CFP | 256 | Cyan | 470/24 | Bandpass | [458-482] | FITC, GFP | 196 | Teal | 510/25 | Bandpass | [497-522] | YFP | 62 | Green/Yellow | 550/15 | Bandpass | [542-557] | TRITC, Cy3 | 260 | Green/Yellow (Storage) | 575/25 | Bandpass | [562-587] | mCherry | 310 | Red | 640/30 | Bandpass | [625-655] | Cy5 | 231 |
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Objectives - 20x/0.5 Air Ph1 WD 2.1
Objectives 20x/0.5 Air Ph1- 60x/1.4 Oil DIC WD 0.13
- 100x/1.45 Oil Ph3 WD 0.13
- 100x/1.45 Oil DIC WD 0.13
Empty- 4x/0.2 Air WD 20
- 20x
\- /0.75 Air
DIC - DIC WD 1.0
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title | Objectives complete specifications |
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Position | Name | Brand | ID | Magnification | Numerical Aperture | Immersion | TypeFull name | Identifier | Working distance (mm) | Transmittance (% [nm]) | TechniqueTechniques | Cover glass thickness (mm) |
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1 | 20x/0.5 Air Ph1 | Nikon | 20x/0.5 Air Plan Fluor | WD 2.1 Ph1? AirPh1 | MRH10201 | 20x | 0.5 | Air | Plan Fluor | 2.1 | >75% >80% [400- | 800750] | BF, Pol, PhC, Fluo | 0.17 | 2 | 60x/1.4 Oil DIC | Nikon | 60x/1.4 Oil Plan Apo Lambda DIC N2 | 60x | 1.4 | MRD01605 | Plan Apo Lambda | 0.13 | >80% [ | 400475- | 750725] | BF, Pol, DIC, Fluo | 0.17 | 3 | | Nikon | 100x/1.45 Oil Plan Apo Lambda Ph3 | 100x | 1.45 | PPlan Apo LambdaMRD31905 | 0.13 | >75% >80% [ | 400475- | 800750] | BF, Pol, | DIC PhC, Fluo | 0.17 | 4 | 100x/1.45 Oil DIC | Nikon | 100x/1.45 Oil Plan Apo Lambda DIC N2 | 100x | 1.45 | MRD01905 | Plan Apo Lambda | 0.13 | >80% [475-750] | BF, Pol, DIC, Fluo | 0.17 | 5 | Empty | 6 | 20x4x/0.75 2 Air DIC | Nikon | 20x4x/0. | 75 2 Air Plan Apo Lambda | DIC N220x | MRD00045 | 20 | >80% [400-1000] | BF, Fluo | 0.17 | 6 | 20x/0.75 Air DIC | Nikon | 20x/0.75 Air Plan Apo Lambda DIC N2 | MRD00205 | 1.0 | >80% [400-950] | 0.75 | Air | Plan Apo Lambda | TBD | BF, Pol, DIC, Fluo | | 0.17 |
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FiltersFilter cubes DAPI /Hoechst/AMCAFITC/EGFP TRITC/Rhodamine TexasRedGFP/FITC (CFP) Cy5 DAPI/GFP/Cy3/Cy5 (requires Cy3 filter in Lumencor SpectraX Green/Yellow position) - DIC Analyzer
CFP/YFP/mCherry (requires mCherry filter in Lumencor SpectraX Green/Yellow position)
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title | Filters complete specifications |
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Semrock | GFP-4050B-000 | 466/40x [446-486] | 495LP | 525/50m
[500-550] | Nikon ID 96372 | 3 | Cy5 | Semrock | Cy5-5070A | 617/55x [590-645] | 652LP | 697/77m [659-736] | Nikon ID 96376 | 4 | DAPI/GFP/Cy3/Cy5 | Semrock | C182279 | None | 409/493/573/652 | 432/515/595/681 | 77074160 Custom Quad C182279. Excitation filters are in the Lumencor SpectraX light source | 5 | DIC Analyzer | Nikon | Ti2-C-DICACL | Not Applicable | Not Applicable | Not Applicable | Used for DIC imaging | 6 | CFP\YFP\mCherry | Semrock | C1997767 | None | 459/526/596 | 475/543/702 | Excitation filters are in the Lumencor SpectraX light source |
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id | User Guide |
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title | User Guide |
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| - Turn on the computer (#1)
- Remove the cover from the microscope
- Turn on the microscope power bar (#2)
If incubation is required, turn on the Okolab incubation module (#3A), the Lauda water bath (#3B) and open the CO2 (#3C) and N2 (#3D) tanks Avertissement |
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Make sure the humidifier and the water bath are clean and properly filled with distilled water. |
- Use your UdeM credentials to log in to Windows
- Start the software
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id | User Guide |
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title | User Guide |
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| UI Expand |
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| - Turn on the computer
- Turn on the microscope power bar
If fluorescence is required, turn on the mercury lamp power supply unit (3A) and press ignition (3B) Avertissement |
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Mercury lamp must be on for at least 30 min before being turned off and vice-versa |
- Log in Windows using your UdM credentials
- Start-up NIS-Elements
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The first time you log in the computeruse the instrument, you need to import the microscope settings into the software. To do this follow the instructions Setting-up NIS-Elementsinstructions First use protocol. |
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| - Save your data
- Close NIS-Elements software
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: driveGet your samples from the microscope
- Turn off the computer
- If oil objectives were used, clean the oil objective it with lens cleaner and lens paper (not Kimwipes)
- If fluorescence incubation was used, turn off the mercury lamp power supply unit (3A)
- Turn off the microscope power bar (2)
- Okolab incubation module (#3A),Lauda water bath (#3B) and close the CO2 (#3C) and N2 (#3D) tanks
- Wait until the SpectaX has cooled down and turn off the microscope power bar (#2)
- Cover Wait until the lamps are cool and cover the microscope
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| - Take back Collect your samples including ones , especially those in the microscope
- Leave the microscope and the working area clean
- Mercury lamp must be on for at least 30 min before being turned off and vice-versa
- workspace clean
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| UI Expand |
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| - Files can be saved temporarily (during acquisition) on the to local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a one folder per laboratory using the principal investigator's last name. WithinInside, create one a folder per user (Firstname_Lastnameusing the following nomenclature (First Name_Last Name).
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| In any case, do not store your files should be removed from the on the C: drive. |
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title | Setting-up NIS-ElementsFirst use |
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| Setting-up NIS-Elements | When using the microscope for Setting-up NIS-Elements | This process is required the first time you are using the instrument, you need to import the microscope settings into the software. You will usually do it this during the training session. It This procedure can also be performed if something is not working properly right or and if you want to refresh reset the software interfaceto its original settings. Remarque |
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This process will delete all experiment protocols and restore the parameters for the reset the software to the original settings for this specific microscope. |
- Close If open, close NIS-Elements
- Wait until the complete closure of NIS-Elements
- Open the folder Desktop\Logiciels
- and wait until it is completely closed (up to 30 seconds)
- On your Desktop open the Softwares folder
- Open Open the software NIS Settings Utility
- Click on the Import tab
- Click on Browse
- Navigate to your desktop Desktop
- Select the file Nikon-E600 Ti2 Settings.bin
- Click Select
- Select all items
- Click Import
- Click OK
- Close the NIS Settings
- Open You can now reopen NIS-Elements
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id | LightpathLight path |
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title | Light path |
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| The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.
Light pathDownload the schematics to follow Transmitted light (Bright field, Phase Contrast, Polarized light) and Reflected light (fluorescence) paths. Light path schematics |
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| Available manuals
Manuals |
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| - Adjust focus drive jitter
- Add a holder for polarized light analyzer
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| - Nikon Preventive Maintenance
- Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
- 100x slightly damaged but not the lens
- Fluorescence cubes damaged: FITC TexasRed
- Focus drive has a jitter
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| - Replacement mercury lamp bulb HBO 1003W/2
- 256 GB SSD added in workstation for OS
- Windows 10 Installation
- BIOS updated to v2.47
- Camera Firmware updated to v2.11
- NIS-Elements Basic Research v4.6 64-bits installed
- Creation NIS Elements Settings
- FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK
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id | Technical Datasheet |
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title | Technical Datasheet |
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| Stand- Nikon Eclipse E600 upright Serial 725540
Light sources- Transmitted light Halogen 12V 100W Serial 01875599
- Reflected light
- Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
- Bulb housing model LH M100C-1 Serial 039326
Condenser- Condenser Universal C-CU Serial 081901
- Lens Dry NA 0.9
- Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty
Objectives- 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
- 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
- 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
- Empty
- Empty
- Empty
Développer |
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title | Objectives complete specifications |
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| Position | Name | Brand | ID | Magnification | Numerical Aperture | Immersion | Type | Working distance (mm) | Transmittance (% [nm]) | Technique | Cover glass thickness (mm) |
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1 | 10x/0.3 Air | Nikon | 10x/0.3 Air Plan Fluor Ph1 DLL | 10x | 0.3 | Air | Plan Fluor | 16 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 | 2 | 40x/0.75 Air | Nikon | 40x/0.75 Air Plan Fluor Ph2 DLL | 40x | 0.75 | Air | Plan Fluor | 0.72 | >80% [400-750] | BF, Pol, PhC, Fluo | 0.17 | 3 | | Nikon | 100x/1.3 Oil Plan Fluor Ph3 DLL | 100x | 1.3 | Plan Fluor | 0.2 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 | 4 | Empty | 5 | Empty | 6 | Empty | FiltersDAPI/Hoechst/AMCA FITC/EGFP TRITC/Rhodamine TexasRed
Développer |
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title | Filters complete specifications |
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| Position | Cube name | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter |
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1 | DAPI/Hoechst/AMCA | Chroma | Cube set 31000v2 | 350/50x [325-375] | 400LP | 460/50m [435-485] | 2 | FITC/EGFP | Chroma | Cube set 41001 | 480/40x [420-500] | 505LP | 535/50m [510-560] | 3 | TRITC/Rhodamine | Chroma | Cube set 41002c | 545/30x [530-560] | 570LP | 620/60m [590-650] | 4 | Texas Red | Chroma | Cube set 41004 | 560/55x [532-588] | 595LP | 645/75m [607-682] | 5 | Empty | 6 | Empty | Detector- Color Camera Nikon DS-Ri2 Serial 701234
Workstation- HP Z440 Workstation
- Intel Xeon E5-1630 v3 @ 3.7GHz
- RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
- OS 256GB SSD 550 MBs
- 2TB HD Data Storage 150 MBs
- Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
- Monitor HP Z24i display 24' 1920x1200
Consumables Tabs Page |
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id | FAQ |
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title | Troubleshooting & FAQ |
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| UI Expand |
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expanded | true |
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title | Troubleshooting |
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| Liquid forming in the incubation chamber This happens when the mixed-gas humidifier is overfilled. Bubbles created by the gas going through the humidification bring liquid into the gas feed line. - Turn off the Okolab module
- Remove your sample and store it properly
- Dry the incubation chamber with a clean tissue
- Carefully remove the cap of the humidifier glass bottle
Avertissement |
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The humidifier bottle is made of glass and is very fragile. Pay extra care when manipulating the humidifier bottle |
- Remove distilled water from the humidifier
Info |
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Humidifier shouldn't be more than 2/3rd filled |
- Close the humidifier by replacing the cap
- Turn on the Okolab module
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| Can I use this microscope to look at cell in a dish? - No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.
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