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Small-angle X-ray scattering (SAXS) is a technique used to derive low resolution structural information about the shape, size, electronic density, molecular weight and conformational changes of macromolecules in solution by taking advantage of the scattering intensities at different angles.

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Tutorial sections

• Link Page
  name SAXS Instrumentation linkText Instrumentation type page

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  • Overview of the instrumentation - to be familiar with the equipment in the laboratory
  • Software - software used by the instrument
  • Maintenance - basic maintenance procedures

• Link Page
  name SAXS experiment linkText SAXS experiment type page

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  • Running a SAXS experiment - first steps into data acquisition
  • SEC-SAXS setup - data acquisition in SEC-SAXS mode

• Link Page
  name SAXS data processing linkText Data processing

  Data processing:
  • Basic processing of SAXS data - initial data analysis and sample quality assessment
  • Advanced processing of SAXS data - calculation of the pairwise distribution function
  • Dealing with SEC-SAXS data - analyzing your SEC-SAXS data

• Link Page
  name Ab initio modeling linkText Ab initio modeling

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  • Modeling with DAMMIF - dummy atom modeling from SAXS data using DAMMIF
  • Modeling with DENSS - electron density modeling from SAXS data using DENSS

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Alternatively, the Structural Biology Platform has computers available where recent versions are installed. Access to the computer room is controlled by access codes. Contact Normand Cyr in order to obtain a code.

Scientific literature

• Jeffries CM, et al (2016). Preparing monodisperse macromolecular samples for successful biological small-Angle X-ray and neutron-scattering experiments. Nature Protocols.
• Putnam CD, et al (2007). X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Quarterly Reviews of Biophysics
• Skou S, et al (2014). Synchrotron-based small-angle X-ray scattering of proteins in solution. Nature Protocols.
• Trewhella J, et al (2017). 2017 publication guidelines for structural modeling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallographica Section D.