Historique de la page

1. Turn on the computer
2. Turn on the microscope power bar

3. If incubation is required, turn on the Okolab incubation module (3A and 3B) and open the CO₂ (3C) and N₂ (3D) tanks

   Avertissement
   Make sure the humidifier and the water bath are clean and properly filled with distilled water.

   
   

4. Log in Windows using your UdM credentials
5. Start-up NIS-Elements

1. Save your data
2. Close NIS-Elements
3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
4. Get your samples from the microscope
5. Clean the oil objectives with lens cleaner and lens paper
6. If incubation was used, turn off the incubation module (3A and 3B) and close the CO₂ (3C) and N₂ (3D) tanks
7. Turn off the microscope power bar (2)
8. Cover the microscope

• Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
• At the end of each session, copy your data to your external drive and delete it from the local C: drive
• You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

This process is required the first time you are using the instrument. You will usually do it during the training session. It can also be performed if something is not working properly right or if you want to refresh the software interface to the original settings.

1. Close NIS-Elements
2. Wait until the complete closure of NIS-Elements
3. Open the folder Desktop\Logiciels
4. Open the software NIS Settings Utility
5. Click on the Import tab
6. Click on Browse
7. Navigate to your desktop
8. Select the file Nikon-Ti2 Settings.bin
9. Click Select
10. Select all items
11. Click Import
12. Click OK
13. Close the NIS Settings
14. Open NIS-Elements

• Bring long Rj45 cable to connect Acquisition and Processing workstations
• Check condenser filters if labels match
• Check why 20x/0.75 DIC objective has no DIC prism
• Condenser DIC prism is DIC N1 while objective requires N2
• Check Lumencor power output
• Check fluorescence fiber injection and fiber coupler adjustments
• Keep an eye on room temperature management
• Fix and/or Replace Okolab free thermal probe
• Check Stage top til adjustment procedure

• 20x/0.75 DIC objective was dirty and has been cleaned
• Printed and displayed a Memo about available air and oil objectives
• Adjustment of camera angle
• Objective calibration
• Objective XY offset
• Updated NIS settings
• Added light path schematics to wikipedia

• Okolab display a NAN message for the free thermal sensor

• The probe is made of two wires that need to be in contact to measure the temperature properly

  • Turn off the Okolab module
  • Disconnect the free sensor probe
  • Cut out the damaged part
  • Carefully expose the two wires
  • Connect the two wires together

• Added label on gas bottles
• Added line mark on humidifier

• 512 GB SSD installed for OS
• Windows 10 Installation
• BIOS updated to v2.47

Liquid forming in the incubation chamber

This happens when the mixed-gas humidifier is overfilled. Bubbles created by the gas going through the humidifier can bring liquid into the gas feed line.

• Turn off the Okolab module
• Remove your sample and store it properly
• Dry the incubation chamber with a clean tissue
• Carefully remove the cap of the humidifier glass bottle

• Remove distilled water from the humidifier

• Close the humidifier by replacing the cap
• Turn on the Okolab module

Can I use this microscope to look at cell in a dish?

• Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
• The objectives are optimized to image through thin glass bottom multi-well plates
• You may also image specimen mounted between a slide and a 0.17mm thick coverslip
• For long timelapse, be aware of photo-toxicity