What is SEC-MALS?

Size Exclusion Chromatography with Multi-Angle Light Scattering analysis (SEC-MALS) is an absolute technique for the determination of the molar mass and the average radius of gyration (R_g) of biomolecules in solution following a separation by liquid chromatography. The technique uses static diffusion of a high intensity light source through a solution containing macromolecules. The intensity of this diffusion is then detected under several angles, hence being able to calculate precisely the molar mass and the average radius of gyration (R_g).

Tutorial sections

• Instrumentation:
  • Overview of the instrumentation
  • Software
  • Available SEC columns
• SEC-MALS experiment
• Data analysis

References for this tutorial

Demonstration data

If you would like to explore this tutorial with demonstration data, you can download this datasets:

• BSA dataset (ZIP file) for the analysis of the molecular weight of a protein.

Websites

• SEC-MALS for absolute mass and size measurements

Software required

• ASTRA