HP Z840 Poste de déconvolution

Roger Gaudry R-619

Applications
- 2D and 3D Deconvolution
- Image processing
- Image Analysis
- Quantification
Processor
- 2 x Intel Xeon E5-2623 v4 2.60 GHz
RAM
- 128 GB (8x16GB) ECC DDR4 2400 MHz
Drives
- 512 GB NVMe at 2500 MB/s OS
- 4 TB (2x2TB) at 170 MB/s spanned volume for storage
Video Card
- NVidia GTX 1080 with 8GB DDR5 dedicated memory
Monitor
- HP Z24n 24" IPS display native resolution 1920 x 1200
OS
- Windows 10 21H1
Softwares
- ImageJ
- FIJI
- Microsoft Office 2016
- NIS-Elements AR v5.02 64-bits
- NIS Elements Offline Deconvolution v4.11 64-bits

Save your data
Close NIS-Elements
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive

Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

In any case, your files should be removed from the C: drive.

Windows 10 UdeM Image installation
Change the 2nd network adapter configuration with fix IP to enable for computer to computer communication
Change sharing policies of public document folder

Transmitted light Halogen 12V 100W Serial 01875599
Reflected light
- Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
- Bulb housing model LH M100C-1 Serial 039326

Position	Name	Brand	ID	Magnification	Numerical Aperture	Immersion	Type	Working distance (mm)	Transmittance (% [nm])	Technique	Cover glass thickness (mm)
1	10x/0.3 Air	Nikon	10x/0.3 Air Plan Fluor Ph1 DLL	10x	0.3	Air	Plan Fluor	16	>75% [400-800]	BF, Pol, PhC, Fluo	0.17
2	40x/0.75 Air	Nikon	40x/0.75 Air Plan Fluor Ph2 DLL	40x	0.75	Air	Plan Fluor	0.72	>80% [400-750]	BF, Pol, PhC, Fluo	0.17
3	100x/1.3 Oil	Nikon	100x/1.3 Oil Plan Fluor Ph3 DLL	100x	1.3	Oil	Plan Fluor	0.2	>75% [400-800]	BF, Pol, PhC, Fluo	0.17
4	Empty
5	Empty
6	Empty

Position	Cube name	Brand	ID	Excitation Filter	Dichroic mirror	Emission Filter
1	DAPI/Hoechst/AMCA	Chroma	Cube set 31000v2	350/50x [325-375]	400LP	460/50m [435-485]
2	FITC/EGFP	Chroma	Cube set 41001	480/40x [420-500]	505LP	535/50m [510-560]
3	TRITC/Rhodamine	Chroma	Cube set 41002c	545/30x [530-560]	570LP	620/60m [590-650]
4	Texas Red	Chroma	Cube set 41004	560/55x [532-588]	595LP	645/75m [607-682]
5	Empty
6	Empty

Deconvolution processing not giving expected results...

This usually happens when the image files do not contain the correct metadata.

Make sure to save the files with all available metadata on the microscope used for the acquisition.
This usually requires to save the files in the software propriatery format (.nd for NIS-Elements and Metamorph, .czi for Zen,...)
You can always add the correct calibration values after acquisition (pixel size in XY and Z, wavelength, Z-step)

Can I use this workstation remotely?

Yes and No. You can't use the software remotely but you can start the software locally and connect remotely to make sure all is running smoothly. To do so you need to request permission to the platform manager.

Which deconvolution method should I use?

There is not a single answer
If you have a single plan you should choose 2D deconvolution. If you have a Z-stack you should pick 3D deconvolution.
If you want to have a quick view of the result you should use Fast Deconvolution.
For best results Landweber deconvolution
For more Yes and No. You can't use the software remotely but you can start the software locally and connect remotely to make sure all is running smoothly. To do so you need to request permission to the platform manager.

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