Fabre L, Ntreh AT, Yazidi A, Leus IV, Weeks JW, Bhattacharyya S, Ruickoldt J, Rouiller I, Zgurskaya HI, Sygusch J. A "Drug Sweeping" State of the TriABC Triclosan Efflux Pump from Pseudomonas aeruginosa.Structure. 2021 Mar 4;29(3):261-274.e6. doi: 10.1016/j.str.2020.09.001. Epub 2020 Sep 22.

Gizak A, Wiśniewski J, Heron P, Mamczur P, Sygusch J*, Rakus D. Targeting moonlighting function of aldolase induces apoptosis in cancer cells. Cell Death Dis. 2019 Sep 26;10(10):712. * Corresponding co-author  

Labiuk SL, Sygusch J*, Grochulski P. Structures of soluble rabbit neprilysin complexed with phosphoramidon or thiorphan. Acta Crystallogr F Struct Biol Commun. 2019 Jun 1;75(Pt 6):405-411. * Corresponding co-author

Arya T, Oudouhou F, Casu B, Bessette B, Sygusch J, Baron C. Fragment-based screening identifies inhibitors of ATPase activity and of hexamer formation of Cagα from the Helicobacter pylori type IV secretion system. Sci Rep. 2019 Apr 24;9(1):6474. doi: 10.1038/s41598-019-42876-6.

Heron P, Abellán-Flos M, Salmon L, Sygusch J. Bisphosphonate inhibitors of mammalian glycolytic aldolase. J Med Chem. 2018 Dec 13;61(23):10558-10572. doi: 10.1021/acs. jmedchem.8b01000. Epub 2018 Dec 3.

Li W, Yazidi A, Pandya AN, Hegde P, Tong W, Calado Nogueira de Moura V, North EJ, Sygusch J, Jackson M. MmpL3 as a Target for the Treatment of Drug-Resistant Nontuberculous Mycobacterial Infections. Front Microbiol. 2018 Jul 10; 9:1547. doi: 10.3389/fmicb.2018.01547. eCollection 2018.

Jacques B, Coinçon M, Sygusch J. Active site remodeling during the catalytic cycle in metal-dependent fructose-1,6-bisphosphate aldolases. J Biol Chem. 2018 May 18;293(20):7737-7753.

Heron P, Sygusch J. Isomer Activation Controls Stereospecificity of Class I FBP Aldolases. J Biol Chem. 2017 Dec 1;292(48):19849-19860.

Oudouhou F, Casu B, Dopgwa Puemi AS, Sygusch J, Baron C. Analysis of Novel Interactions between Components of the Selenocysteine Biosynthesis Pathway, SEPHS1, SEPHS2, SEPSECS, and SECp43. Biochemistry. 2017 May 2;56(17):2261-2270.

Wahba H, Stevenson M, Mansour A, Sygusch J, Wilcox D, Omichinski J. Structural and biochemical characterization of organotin and organolead compounds binding to the organomercurial lyase MerB provide new insights into its mechanism of carbon-metal bond cleavage. J Am Chem Soc. 2017 Jan 18;139(2):910-921.

Casu B, Smart J, Hancock MA, Smith M, Sygusch J, Baron C. Structural analysis and inhibition of TraE from the pKM101 type IV secretion system. J Biol Chem. 2016 Nov 4;291(45):23817-23829.

Briot J, D’Avanzo N, Sygusch J, Parent, L. Three-dimensional architecture of the L-type calcium channel: Structural insights into the CaV 2 1 auxiliary protein. Biochem Mol Biol Journal, 2016 2(3), 16.

Belardinelli JM#, Yazidi A#, Yang L, Fabre L, Li W, Jacques B, Angala SK, Rouiller I, Zgurskaya HI, Sygusch J*, Jackson M*. Structure-function profile of MmpL3, the essential mycolic acid transporter from Mycobacterium tuberculosis. ACS Infectious Diseases 2016 Oct 14; 2(10):702-713. Epub 2016 Sep 1 * Corresponding co-author   # Equal contributions

Wahba HM, Lecoq L, Stevenson M, Mansour A, Cappadocia L, Lafrance-Vanasse J, Wilkinson KJ, Sygusch J, Wilcox DE, Omichinski JG. Structural and Biochemical Characterization of a Copper-Binding Mutant of the Organomercurial Lyase MerB: Insight into the Key Role of the Active Site Aspartic Acid in Hg-Carbon Bond Cleavage and Metal Binding Specificity. Biochemistry. 2016 Feb 23;55(7):1070-81.

Tanner JE, Coinçon M, Leblond V, Hu J, Fang JM, Sygusch J, Alfieri C. Peptides Designed To Spatially Depict the Epstein-Barr Virus Major Virion Glycoprotein gp350 Neutralization Epitope Elicit Antibodies That Block Virus-Neutralizing Antibody 72A1 Interaction with the Native gp350 Molecule. J Virol. 2015 May 1;89(9):4932-41.

Cappadocia L, Parent JS, Sygusch J*, Brisson N. A family portrait: structural comparison of the Whirly proteins from Arabidopsis thaliana and Solanum tuberosum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11): 1207-11. Epub 2013 Oct 26. *Corresponding co-author

Smith MA, Coinçon M, Paschos A, Jolicoeur B, Lavallée P, Sygusch J, Baron C. (2012) Identification of the Binding Site of Brucella VirB8 Interaction Inhibitors. Chem Biol. Aug 24;19(8):1041-8.

Coincon M, Wang W, Sygusch J*, Seah SY. (2012) Crystal structure of reaction intermediates in pyruvate Class II aldolase:  Substrate Cleavage, Enolate Stabilization and Substrate Specificity. J Biol Chem. 287(43):36208-21. Epub 2012 Aug 20. *Corresponding co-author

Cappadocia L, Parent JS, Zampini E, Lepage E, Sygusch J*, Brisson N. A conserved lysine residue of plant Whirly proteins is necessary for higher order protein assembly and protection against DNA damage. Nucleic Acids Res. 2012 40(1):258-69. *Corresponding co-author

de la Paz Santangelo M, Gest PM, Guerin ME, Coinçon M, Pham H, Ryan G, Puckett SE, Spencer JS, Gonzalez-Juarrero M, Daher R, Lenaerts AJ, Schnappinger D, Therisod M, Ehrt S, Sygusch J, Jackson M. Glycolytic and Non-glycolytic Functions of Myco-bacterium tuberculosis Fructose-1,6-bis-phosphate Aldolase, an Essential Enzyme Produced by Replicating and Non-replicating Bacilli. J Biol Chem. 2011 Nov 18; 286(46): 40219-31.

Gabin CMB, Arthus-Cartier G, Hannaert V, Thérisod H, Sygusch J*, Thérisod M*. (2011) Mannitol based inhibitors of fructose 1,6-bisphosphate Schiff-base forming aldolase from Trypanosoma brucei. ACS Med. Chem. Lett., 2(11), 804–808. *Corresponding co-author

Daher R, Coinçon M, Fonvielle M, Gest PM, Guerin ME, Jackson M, Sygusch J*, Therisod M. Rational design, synthesis, and evaluation of new selective inhibitors of microbial class II (zinc dependent) fructose bis-phosphate aldolases. J Med Chem. 2010 53(21):7836-42 *Corresponding co-author

Cappadocia L, Maréchal A, Parent JS, Lepage E, Sygusch J*, Brisson N. Crystal structures of DNA-Whirly complexes and their role in Arabidopsis organelle genome repair. Plant Cell. 2010 22:1849-67. Epub 2010 Jun 15. *Corresponding co-author

Low-Kam C, Liotard B, Sygusch J. (2010) Structural study into apparent loss of stereo-specificity by a class I tagatose-1,6-bis-phosphate aldolase. J. Biol. Chem. April 28, 2010 285:21143-52. Epub 2010 Apr 28.

Park HJ, Rangarajan ES, Sygusch J*, Izard, T. (2010) Dramatic Improvement of Crystal Quality for Low Temperature-grown Rabbit Muscle Aldolase.  Acta Cryst F: Struct. Biol. Cryst. Comm., 66, 595-600. * Corresponding author

Rangarajan ES, Park H, Fortin E, Sygusch J*, Izard T. Mechanism of aldolase control of sorting nexin 9 function in endocytosis. (2010) J Biol Chem. 285, 11983-90. Epub 2010 Feb 2 * Corresponding author

St-Jean M, Blonski C, Sygusch J. Charge Stabilization and Entropy Reduction of Central Lysine Residues in Fructose-Bisphosphate Aldolase. Biochemistry. 2009 48(21): 4528-4537. Apr 22. [Epub ahead of print]

Starnes GL, Coincon M, Sygusch J, Sibley LD. Aldolase is essential for energy production and bridging adhesin-actin cytoskeletal interactions during parasite invasion of host cells. Cell Host Microbe. 2009 Apr 23;5(4):353-64.

Lafrance-Vanasse J, Lefebvre M, Di Lello P, Sygusch J, Omichinski JG Crystal Structures of the Organomercurial Lyase MerB in Its Free and Mercury-bound Forms: INSIGHTS INTO THE MECHANISM OF METHYLMERCURY DEGRADATION. J Biol Chem. 2009 Jan 9;284(2):938-44. Epub 2008 Nov 12

Cappadocia L, Sygusch J, Brisson N. Purification, crystallization and preliminary X-ray diffraction analysis of the Whirly domain of StWhy2 in complex with single-stranded DNA. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1056-9. Epub 2008 Oct 3

Fonvielle M, Coinçon M, Daher R, Desbenoit N, Kosieradzka K, Barilone N, Gicquel B, Sygusch J, Jackson M and Therisod M. Synthesis and Biochemical Evaluation of Selective Inhibitors of Class II Fructose bis-Phosphate Aldolases: Towards New Synthetic Antibiotics  (2008). Chem. 14, 8521-8529.

Mabiala-Bassiloua CG, Zwolinska M, Therisod H, Sygusch J, Therisod. Separate synthesis and evaluation of glucitol bis-phosphate and mannitol bis-phosphate, as competitive inhibitors of fructose bis-phosphate aldolases. Bioorg Med Chem Lett. 2008 Mar 1;18(5):1735-7. Epub 2008 Jan 26.

St-Jean, M., Sygusch JJ. Stereospecific proton transfer by a mobile catalyst in mammalian fructose-1,6-bisphosphate aldolase. (2007) J. Biol. Chem. Oct 19;282(42):31028-37. Epub 2007 Aug 29.
Lafrance-Vanasse J, Sygusch J. Carboxy-Terminus Recruitment Induced by Substrate Binding in Eukaryotic Fructose Bis-phosphate Aldolases. Biochemistry 2007 46(33):9533-40.

St-Jean M, Izard T, Sygusch J. A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein. J. Biol. Chem. 2007 282, 14309-14315.

Dax C, Duffieux F, Chabot N, Coincon M, Sygusch J, Michels PA, Blonski C. Selective irreversible inhibition of fructose 1,6-bisphosphate aldolase from Trypanosoma brucei. J Med Chem. 2006 Mar 9;49(5):1499-502.

St-Jean M, Lafrance-Vanasse J, Liotard B, Sygusch J. High resolution reaction intermediates of rabbit muscle fructose-1,6-bisphosphate aldolase: substrate cleavage and induced fit. J Biol Chem. 2005 Jul 22;280(29):27262-70. Epub 2005 May 3.

Dax C, Coincon M, Sygusch J, Blonski C. Hydroxynaphthaldehyde phosphate derivatives as potent covalent Schiff base inhibitors of fructose-1,6-bisphosphate aldolase. Biochemistry. 2005 Apr 12;44(14):5430-43.

Liotard B, Sygusch J. Purification, crystallization and preliminary X-ray analysis of native and selenomethionine class I tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes. Acta Crystallogr D Biol Crystallogr. 2004 Mar;60 (Pt 3):528-30. Epub 2004 Feb 25.

Izard T, Sygusch J. Induced fit movements and metal cofactor selectivity of class II aldolases: structure of Thermus aquaticus fructose-1,6-bisphosphate aldolase. J Biol Chem. 2004 Mar 19;279(12):11825-33. Epub 2003 Dec 29.

Azema L, Lakhdar-Ghazal F, Sygusch J, Blonski C. Characterization of proton abstraction steps in enzymatic reactions by Fourier transform infrared spectroscopy. Anal Biochem. 2003 Jul 1;318(1):142-5.

Kawaji M, Gamache O, Hwang DH, Ichikawa N, Viola JP, Sygusch J. Investigation of Marangoni and natural convection during protein crystal growth. J. Crystal Growth, 2003 258, 420–430.

Desveaux D, Allard J, Brisson N, Sygusch J. A new family of plant transcription factors displays a novel ssDNA-binding surface. Nat Struct Biol. 2002 Jul;9(7):512-7.

Desveaux D, Allard J, Brisson N, Sygusch J. Crystallization and preliminary X-ray crystallographic analysis of p24, a component of the potato nuclear factor PBF-2. Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):296-8. Epub 2002 Jan 24.

Maurady A, Zdanov A, de Moissac D, Beaudry D, Sygusch J. A conserved glutamate residue exhibits multifunctional catalytic roles in D-fructose-1,6-bisphosphate aldolases. J Biol Chem. 2002 Mar 15;277(11):9474-83. Epub 2002 Jan 4.

Allard J, Grochulski P, Sygusch J. Covalent intermediate trapped in 2-keto-3-deoxy-6- phosphogluconate (KDPG) aldolase structure at 1.95-A resolution. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3679-84.

Yoon TS, Tétreault S, Bosshard HE, Sweet RM, Sygusch J. Mosaic spread analysis of Canadian advanced protein crystallization experiment on the Russian space station, Mir. J. Cryst. Growth, 2001 232, 520-535.

Sauve V, Sygusch J. Molecular cloning, expression, purification, and characterization of fructose-1,6-bisphosphate aldolase from Thermus aquaticus. Protein Expr Purif. 2001 Mar;21(2):293-302.

Sauve V, Sygusch J. Crystallization and preliminary X-ray analysis of native and selenomethionine fructose-1,6-bisphosphate aldolase from Thermus aquaticus. Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):310-3.

Rellos P, Sygusch J, Cox TM. Expression, purification, and characterization of natural mutants of human aldolase B. Role of quaternary structure in catalysis. J Biol Chem. 2000 Jan 14;275(2):1145-51.

Todd P, Doyle JF, Carter P, Wachtel H, Deuser MS, Vellinger JC, Cassanto JM, Alvarado U, Sygusch J, Kent TB. Magnetic space shuttle experiments. J. Magn. Magnetic Materials. 1999 194, 96-101.

Rellos P, Ali M, Vidailhet M, Sygusch J, Cox TM. Alteration of substrate specificity by a naturally occurring aldolase B mutation (Ala337-->Val) in fructose intolerance. Biochem J. 1999 May 15;340 ( Pt 1):321-7.

Ethier N, Talbot G, Sygusch J. Gene cloning, DNA sequencing, and expression of thermostable beta-mannanase from Bacillus stearothermophilus. Appl Environ Microbiol. 1998 Nov;64(11):4428-32.

Sygusch J, Beaudry D. Allosteric communication in mammalian muscle aldolase. Biochem J. 1997 Nov 1;327 ( Pt 3):717-20.

Sygusch J, Beaudry D. Subunit interaction in mammalian aldolases. Biochem J. 1997 May 1;323 ( Pt 3):671-6.

Blonski C, De Moissac D, Perie J, Sygusch J. Inhibition of rabbit muscle aldolase by phosphorylated aromatic compounds. Biochem J. 1997 Apr 1;323 ( Pt 1):71-7.

Blom NS, Sygusch J. High resolution fast quantitative docking using Fourier domain correlation techniques. Proteins. 1997 Apr;27(4):493-506.

Blom N, Sygusch J. Product binding and role of the C-terminal region in class I D-fructose1,6-bisphosphate aldolase. Nat Struct Biol. 1997 Jan;4(1):36-9.

De Montigny C, Sygusch J. Functional characterization of an extreme thermophilic class II fructose-1,6-bisphosphate aldolase. Eur J Biochem. 1996 Oct 1;241(1):243-8.

Blom NS, Tetreault S, Coulombe R, Sygusch J. Novel active site in Escherichia coli fructose 1,6-bisphosphate aldolase. Nat Struct Biol. 1996 Oct;3(10):856-62.

Sygusch J, Coulombe R, Cassanto JM, Sportiello MG, Todd P. Protein crystallization in low gravity by step gradient diffusion method. J Cryst Growth. 1996 May 1;162(3-4):167-72.

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