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Button Hyperlink
iconbuild
titleFrançais
typeprimary
urlLeica DM2000


Tabs Container
idInstrument Tabs
titleLeica DM2000
directionhorizontal


Tabs Page
idDescription
titleDescription


Leica DM2000 Upright Microscope


Desmarais Building, Room 3248
Available upon request to Dr Samaha Lab


  • Applications

    • Bright-field

    • Fluorescence
    • Color camera

  • Light sources

    • Halogen lamp 30 W for transmitted light

    • Mercury lamp 100 W (350~600nm) for fluorescence


Développer
titleMercury lamp complete specifications


Peak Wavelength (nm)Power (mW)
3347
36545
40534
43643
54637
57926

HBO Mercury lamp emission spectra (Source)
Comparison HBO Mercury vs XCite Metal Halide Lamps (Source)
OSRAM HBO 100W/2 Mercury lamp manual (pdf)

  • Objectives

    1. 10x/0.3 Air WD 11

    2. 20x/0.5 Air WD 1.15

    3. 40x/0.75 Air WD 0.37

Développer
titleObjectives complete specifications


PositionNameBrandFull nameProduct IDMagnificationNumerical apertureImmersionTypeWorking distance (mm)Transmittance
(% [nm])		TechniqueCoverglass thickness (mm)
110x/0.3 AirLeica10x/0.3 Air HC Plan Fluotar 500650510x0.3AirPlan Fluor11

>80% [385-775]
Max 95% @550nm
Transmittance curve

BF, Fluo0.17
2

20x/0.5 Air

Leica20x/0.5 Air HC Plan Fluotar 50650320x

0.5

Air

Plan Fluor1.15

>80% [395-800]
Max 90% @530nm
Transmittance curve

BF, Fluo0.17
3

40x/0.75

Leica

40x/0.75 Air HC Plan Fluotar

506144

40x

0.75

Air

Plan Fluor0.37

>80% [420-850]
Max 89% @550nm
Transmittance curve

BF, Fluo0.17
4Empty










5Empty










6Empty












  • Filter cubes
    • I3 (GFP, YFP)
    • N2.1 (TRITC)
Développer
titleFilters complete specifications


PositionNameBrandIDExcitation  filterDichroic mirrorEmission filterComments
1I3LeicaI3470/40
[450-490]		510LP515LP
[520+]		Emission filter is very broad.
Be aware of bleed-through.
2N2.1LeicaN2.1

537/45
[515-560]

580LP

590LP
[595+]		Emission filter is very broad.
Be aware of bleed-through.
3Empty






4Empty






5Empty






  • Detector
    • Color camera Leica DFC425 C 2592 × 1944 pixels, 14-bit in monochrome mode , 36-bit in color mode, 6 image/s at full resolution


Tabs Page
idUser Guide
titleUser Guide


UI Expand
expandedtrue
titleStart-up
  1. Turn on the computer (#1)

  2. If fluorescence is required, turn on the mercury lamp power supply unit (#2)

    Avertissement

    Mercury lamp must be on for at least 30 min before being turned off and vice-versa


  3. If transmitted light is required, turn on the halogen lamp on the right side of the microscope (#3)
  4. Log in Windows using your UdM credentials
  5. Start-up LAS 
Info

The first time you use LAS, you will be asked for registration and activation. 

  1. Select Do not tell me about this again at the bottom left
  2. Click Close



UI Expand
titleShutdown
  1. Save your data
  2. Close LAS
  3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  4. Turn off the computer
  5. If fluorescence was used, turn off the mercury lamp power supply unit (#2)
  6. If transmitted light was used, turn off the halogen lamp on the right side of the microscope (#3)

  7. Wait until the lamps have cooled and cover the microscope

Remarque
titleImportant Reminders
  • Take back your samples including ones in the microscope
  • Leave the microscope and the working area clean
  • Mercury lamp must be on for at least 30 min before being turned off and vice-versa



UI Expand
titleStorage management
  • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from the local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
Remarque

In any case, your files should be removed from the C: drive.



UI Expand
titleSoftware setup

The first time you use LAS, you will be asked for registration and activation. 

  1. Select Do not tell me about this again at the bottom left
  2. Click Close



Tabs Page
idLightpath
titleLightpath


The following schematics depict the lightpath for transmitted (bright-field) and reflected (fluorescence) lights.

Leica DM2000 Lightpath (pdf)


Tabs Page
idManuals
titleManuals


Available manuals


Tabs Page
idLog
titleLog


UI Expand
expandedtrue
titleTo do
  • Bring test slides


UI Expand
title2022-03-12
  • Added to Wiki
  • Computer replacement
  • Windows 10 installation
  • Network connection



Tabs Page
idTechnical datasheet
titleTechnical datasheet

Stand

  • Leica DM2000 upright microscope Serial 3312222-022011
  • Leica Trinocular 11551511 BZ00
  • Camera Adapter Leica 11541544

Light sources

  • Transmitted Halogen 30W 
  • Reflected light

    • Power supply Ebq 100-04-L Serial 11 1111 0103B00931

    • Leica Mercury lamp housing Product ID 11504114

Condenser

  • TBD

  • Condenser filters

    • DF Filters 501158 (looks like phase ring)

Objectives

  • Nikon Plan Fluor 4x/0.13 MRH000041 (should not be used)

  • Leica HCX PL Fluotar 10x/0.3 506505

  • Leica HCX PL Fluotar 20x/0.5 506503

  • Leica HCX PL Fluotar 40x/0.75 506144

Stage

  • Manual Stage TBD

Filters

  1. Leica I3
  2. Leica N2.1
  3. Empty
  4. Empty
  5. Empty
  6. Empty

Cube Leica 11513882

Detector

  • Leica DFC425C Serial 535111411 Color Camera 2592 × 1944 pixels, 14-bit monochrome, 36-bit color mode, 6 image/s at full resolution

Workstation

  • HP Z440 Asus P6X58D-E Workstation
  • Intel Xeon E5-1620 v4 @ 3.5GHz
  • Intel Intel Core i7-950 @ 3.0 GHz
  • RAM 6 GB DDR3 1333 MHz (3 x 2 RAM 32 GB DDR4 2400 MHz ECC (4 x 8 GB)
  • OS 500GB 500 GB SSD 550 MB/s
  • 4TB 1 TB HD Data Storage (2 4 x 2 TB 250 GB spanned volume) 170 TBD MB/s
  • Video Card nVidia GTX 1080 8GB AMD Radeon HD 5670 1 GB DDR5 dedicated memory
  • Monitor HP Z24i display 24' 1920x1200
  • Monitor Viewsonic VX922 19' 1280 x 1024
  • Software LASSoftware NIS-Elements AR v5.02

Incubation

  •  None

Consumables

  • Halogen bulb 30 W model TBD
  • HBO 100W/2 Mercury lamp bulb


Tabs Page
idDépannage et Troubleshooting and FAQ
titleDépannage et Troubleshooting and FAQ


Troubleshooting

expanded
UI Expand
truetitleDépannageI don't see the fluorescence

This can happen when something is not setup properly along the lightpath. The lightpath schematics can guide to identify the issue.

  1. Ensure the mercury lamp power supply (#2) is turned ON
  2. Ensure the mercury lamp located at the back of the microscope is shining. It should generate some heat. Be carefull of burning
  3. Ensure the neutral density filters

La fluorescence est allumée mais je ne vois aucune lumière à l'échantillon

Ceci peut survenir lorsqu'un élément n'est pas dans la bonne configuration le long du trajet lumineux. Les schémas du trajet lumineux peuvent vous aider à identifier le problème. 

  1. Vérifiez que l'alimentation de la lampe au mercure est allumé (#2)
  2. Vérifiez que la lampe au mercure située en haut à l'arrière du microscope est allumée. Elle dégage une forte chaleur. Attention aux risques de brulure.
  3. Vérifiez que les filtres de densité neutre (N16, N4, N2) situés en arrière sur le coté droit du microscope sont en position basse (out)
  4. Vérifiez que l'obturateur manuel situé en arrière sur le coté droit du microscope est en position basse (out)
  5. Vérifiez que les diaphragmes d'ouverture (A) et de champ (F) situés en arrière sur le coté droit du microscope sont ouverts en position haute
  6. located at the back on the right side of the microscope are out (low position)
  7. Ensure the manual shutter located at the back on the right side of the microscope is open (low position)
  8. Ensure the aperture (A) and field (F) diaphragms located at the back on the right side of the microscope are opened (high position)
  9. Ensure the filter cube is on a non-empty position Vérifiez que la tourelle de cube est sur une position non vide (1: I3 ou 2: N2.1)


FAQ

UI Expand
titleFAQCan I use this microscope to look at cells in culture

No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip

Puis-je utiliser ce microscope pour observer des cellules en culture?

Non. C'est un microscope droit conçu pour l'observation de spécimens montés entre lame et lamelles (d'épaisseur 0.17mm)

.





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titleLeica DM2000
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titleImage de démonstration





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