- Created by Nicolas Stifani, last modified on Feb 07, 2024
Leica DM2000 Upright Microscope
P-G Desmarais Building, Room 3248
Available upon request to Dr Samaha Lab
- Applications
Bright-field
- Fluorescence
- Color camera
Light sources
Halogen lamp 30 W for transmitted light
Mercury lamp 100 W (350~600 nm) for fluorescence
Peak emission (nm) | Power (mW) |
---|---|
334 | 7 |
365 | 45 |
405 | 34 |
436 | 43 |
546 | 37 |
579 | 26 |
HBO Mercury lamp emission spectra (Source)
Comparison HBO Mercury vs XCite Metal Halide Lamps (Source)
OSRAM HBO 100W/2 Mercury lamp manual (pdf)
Objectives
10x/0.3 Air WD 11
20x/0.5 Air WD 1.15
40x/0.75 Air WD 0.37
Position | Name | Brand | Full name | ID | Magnification | Numerical aperture | Immersion | Type | Working distance (mm) | Transmittance (% [nm]) | Technique | Coverglass thickness (mm) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 10x/0.3 Air | Leica | 10x/0.3 Air HC Plan Fluotar | 5006505 | 10x | 0.3 | Air | Plan Fluor | 11 | >80% [385-775] | BF, Fluo | 0.17 |
2 | 20x/0.5 Air | Leica | 20x/0.5 Air HC Plan Fluotar | 506503 | 20x | 0.5 | Air | Plan Fluor | 1.15 | >80% [395-800] | BF, Fluo | 0.17 |
3 | 40x/0.75 | Leica | 40x/0.75 Air HC Plan Fluotar | 506144 | 40x | 0.75 | Air | Plan Fluor | 0.37 | >80% [420-850] | BF, Fluo | 0.17 |
4 | Empty | |||||||||||
5 | Empty | |||||||||||
6 | Empty |
- Filter cubes
- I3 (GFP, YFP)
- N2.1 (TRITC)
Position | Name | Brand | ID | Excitation filter | Dichroic mirror | Emission filter | Comments |
---|---|---|---|---|---|---|---|
1 | I3 | Leica | I3 | 470/40 [450-490] | 510LP | 515LP [520+] | Emission filter is very broad. Be aware of bleed-through. |
2 | N2.1 | Leica | N2.1 | 537/45 | 580LP | 590LP [595+] | Emission filter is very broad. Be aware of bleed-through. |
3 | Empty | ||||||
4 | Empty | ||||||
5 | Empty |
- Detector
- Color camera Leica DFC425C 2592 × 1944 pixels, 14-bit in monochrome mode , 36-bit in color mode, 6 images/s at full resolution
- Color camera Leica DFC425C 2592 × 1944 pixels, 14-bit in monochrome mode , 36-bit in color mode, 6 images/s at full resolution
- Turn on the computer (#1)
If fluorescence is required, turn on the mercury lamp power supply unit (#2)
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
- If transmitted light is required, turn on the halogen lamp on the right side of the microscope (#3)
- Log in Windows using your UdM credentials
- Start-up LAS
The first time you use LAS, you will be asked for registration and activation.
- Select Do not tell me about this again at the bottom left
- Click Close
- Save your data
- Close LAS
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
- Turn off the computer
If fluorescence was used, turn off the mercury lamp power supply unit (#2)
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
If transmitted light was used, turn off the halogen lamp on the right side of the microscope (#3)
Wait until the lamps have cooled and cover the microscope
Important Reminders
- Take back your samples including ones in the microscope
- Leave the microscope and the working area clean
- Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
In any case, your files should be removed from the C: drive.
The first time you use LAS, you will be asked for registration and activation.
- Select Do not tell me about this again at the bottom left
- Click Close
The following schematics depict the light path for transmitted (bright-field) and reflected (fluorescence) lights.
Available manuals
- None
- Test slides made and brought to the microscope
- Added to Wiki
- Computer replacement
- Windows 10 installation
- Network connection
- Mercury lamp alignment
Stand
- Leica DM2000 upright microscope Serial 3312222-022011
- Leica Trinocular 11551511 BZ00
- Camera Adapter Leica 11541544
Light sources
- Transmitted Halogen 12 V 30 W P/N 11038101180000
- Reflected light
Power supply Ebq 100-04-L Serial 11 1111 0103B00931
Leica Mercury lamp housing Product ID 11504114
Condenser
- Leica 11501183 BZ:02
Condenser filters
- DF Filters 501158 (looks like phase ring)
Objectives
- Nikon Plan Fluor 4x/0.13 MRH000041 (should not be used)
Leica HCX PL Fluotar 10x/0.3 506505
Leica HCX PL Fluotar 20x/0.5 506503
Leica HCX PL Fluotar 40x/0.75 506144
- Empty
- Empty
Stage
- Manual Stage #11888186
Filters
- Leica I3
- Leica N2.1
- Empty
- Empty
- Empty
Cube Leica 11513882
Detector
- Leica DFC425C Serial 535111411 Color Camera 2592 × 1944 pixels, 14-bit monochrome, 36-bit color mode, 6 image/s at full resolution
Workstation
- Asus P6X58D-E Workstation
- Intel Intel Core i7-950 @ 3.0 GHz
- RAM 6 GB DDR3 1333 MHz (3 x 2 GB)
- OS 500 GB SSD 382 MB/s
- 1 TB HD Data Storage (4 x 250 GB spanned volume) 85 MB/s
- Video Card AMD Radeon HD 5670 1 GB DDR5 dedicated memory
- Monitor Viewsonic VX922 19' 1280 x 1024
- Software LAS
Incubation
- None
Consumables
- Halogen bulb 12 V 30 W OSRAM 64261 HLX
- Mercury lamp 100 W OSRAM HBO 103W/2
Troubleshooting
This can happen when something is not setup properly along the light path. The light path schematics can guide to identify the issue.
- Ensure the mercury lamp power supply (#2) is turned ON
- Ensure the mercury lamp located at the back of the microscope is shining. It should generate some heat. Beware of burn risks
- Ensure the neutral density filters (N16, N4, N2) located at the back on the right side of the microscope are out (low position)
- Ensure the manual shutter located at the back on the right side of the microscope is open (low position)
- Ensure the aperture (A) and field (F) diaphragms located at the back on the right side of the microscope are opened (high position)
- Ensure the filter cube is on a non-empty position (1: I3 ou 2: N2.1)
FAQ
No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.