X-Cite 120Q Brochure (pdf)
X-Cite 120Q quick start guide (pdf)
X-Cite 120Q user guide (pdf)
Comparison between mercury and X-Cite emission spectra (Source)

1. Turn on the computer (#1)
2. Turn on the microscope power bar (#2)
3. Press the power witch on the left side of the microscope (#3)

4. If fluorescence is required, turn on the X-Cite lamp (#4A) and open the diaphragm (#4B)

   Avertissement
   The X-Cite lamp must be on for at least 30 min before being turned off and vice-versa

   
   

5. Log in Windows using your UdM credentials
6. Start-up Zen Blue

1. Save your data
2. Close Zen Blue
3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
4. Turn off the computer

5. If fluorescence was used, turn off the X-Cite lamp (#4A)

   Avertissement
   The X-Cite lamp must be on for at least 30 min before being turned off and vice-versa

   
   

6. Turn off the microscope power bar (#2)

7. Cover the microscope

• Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
• At the end of each session, copy your data to your external drive and delete it from the local C: drive
• You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

The first time you use the instrument, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

1. If open, close Zen Blue and wait until it is completely closed (up to 30 seconds)
2. On your Desktop open the Documentation folder
3. Double click on Zen Settings for Axio-Observer Z1
4. You can now reopen Zen Blue

• Nothing

• Added complete description

• Added to wiki

This microscope is motorized for most of its components, yet the X-Cite light source is manual. Ensure the X-Cite diaphragm (#4B) fully opened (high position).

1. On the X-Cite lamp
2. Turn the intensity diaphragm (#4B) up

• Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
• The objectives are optimized to image through thin glass bottom multi-well plates
• You may also image specimen mounted between a slide and a 0.17mm thick coverslip
• For long timelapse, be aware of photo-toxicity

Yes, but... This microscope has an incubation module to maintain temperature, humidity and gas. Yet it does not have a Definite focus which can maintain focus throughout time. Therefore, it is possible to loose the focus over long period.