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id | Description |
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title | Description |
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| Zeiss Axio-Observer Z1 inverted microscopeRoger Gaudry Building, Room R-421 Simple Microscope usage price Access upon request to the platform manager. Consult the Link in New Window |
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linkText | fees structure |
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href | https://pharmacologie-physiologie.umontreal.ca/wp-content/uploads/sites/38/2017/05/tarification-2017.pdf |
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| and Link in New Window |
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linkText | access policies |
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href | https://pharmacologie-physiologie.umontreal.ca/ressources/plateforme-de-microscopie/politique-dacces/ |
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| of the Link in New Window |
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linkText | Phamarcology and Physiology Department Microscopy Platform |
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href | https://pharmacologie-physiologie.umontreal.ca/ressources/plateforme-de-microscopie/ |
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| .Instrument awarded to Dr. Audrey Claing and Jean-Philippe Gratton by the Canadian Foundation for Innovation (CFI) - Applications
- Bright-field
- Phase contrast
- Fluorescence
Light sources
Objectives 2.5x/0.085 Air WD 8.8 - Empty
10x/0.25 Air Ph1 WD 6.5 - Empty
- 20x/0.5 Air Ph2 WD 2.0
- 40x/0.95 Air WD 0.25
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title | Objectives complete specifications |
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Position | Name | Brand | Full name | ID | Magnification | Numerical Aperture | Immersion | Type | Working distance (mm) | Transmittance (% [nm]) | Technique | Cover glass thickness (mm) |
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1 | 20x/0.5 Air | Zeiss | 20x/0.50 Ph2 EC Plan-Neofluar M27 | 420351-9910 | 20x | 2.0 | Air | Plan Neofluar | 2.0 | Not Available | BF, PhC, Fluo | 0.17 | 2 | Empty |
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| 3 | | Zeiss | 40x/0.95 Plan-Apochromat Corr M27 | 420660-9970 | 40x | 0.95 | | Plan ApoChromat | 0.25 | >80% [400-840] | BF, Fluo | 0.13 - 0.21 Correction ring | 4 | Empty |
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| 5 | 2.5x/0.085 Air | Zeiss | 2.5x/0.085 EC Plan-Neofluar M27 | 420320-9902 | 2.5x | 0.085 | Air | Plan Neofluar | 8.8 | >95% [400-750] | BF, Fluo | 0.17 | 6 | 10x/0.25 Air | Zeiss | 10x/0.25 Ph1 N-Achroplan M27 | 420941-9911 | 10x | 0.25 | Air | AchroPlan | 6.5 | Not available | BF, PhC, Fluo | 0.17 |
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- Filter cubes
- DAPI
- GFP
- Rhodamine
- DHE (dihydroethidium)
- Cy5
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title | Filters complete specifications |
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- Détector
- Zeiss AxioCam MR R3 CCD Camera 1388 x 1040 pixels, 12-bit, 13 images/s at full resolution, detector size 8.9 mm x 6.7 mm
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id | User Guide |
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title | User Guide |
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expanded | true |
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title | Start-upStartup |
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| Remove the dust cover from the microscope Turn on the computer (#1) Turn on the microscope power bar at the bak f the computer monitor (#2) (#4BPress the power witch microscope start button located on the rear left side of the microscope (#3 ) If fluorescence is required, turn on the X-Cite lamp (#4A) and open the diaphragm) Avertissement | The X-Cite lamp must be on for at least 30 min before being turned off and vice-versa- Log in Windows using your UdM credentials
- Start-up Zen Blue
Info | The first time you use the instrument, you need Remarque |
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When using for the first time, it is necessary to import the microscope |
settings into -specific parameters BEFORE starting the software. |
To do this follow the instructions Software setup. See the First Use section below. |
- Startup Zen Blue
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| Save your dataClose Zen BlueTransfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: driveTurn off the computerIf fluorescence was used, turn off the X-Cite lamp (#4A) Avertissement |
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The X-Cite lamp must be on for at least 30 min before being turned off and vice-versa |
Turn off the microscope power bar (#2) Cover the microscope Remarque |
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| - Take back your samples including ones in the microscope
- Leave the microscope and the working area clean
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| When using for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example. Remarque |
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Please note, this procedure will delete all your experiment protocols and restore the software to its original settings. |
- If open, close the Zen Blue software and wait for it to close completely (up to 30 seconds)
- On the Desktop open the Documentation folder
- Double-click Settings for Axio-Osberver Z1
- A script will run and a black window will appear briefly
- You can then reopen the Zen Blue software
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| - Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
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In any case, your files should be removed from the C: drive. |
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title | Software setup | Shutdown |
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| - Save your data
- Close Zen Blue
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
Turn off the microscope power bar (#2) - Turn off the computer
- Cover the microscope
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| - Take back your samples including ones in the microscope
- Leave the microscope and the working area clean
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The first time you use the instrument, you need to import the microscope settings into the software. You will usually do this during the training session. This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings. Remarque |
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This process will delete all experiment protocols and reset the software to the original settings for this specific microscope. | If open, close Zen Blue and wait until it is completely closed (up to 30 seconds)On your Desktop open the Documentation folderDouble click on Zen Settings for Axio-Observer Z1You can now reopen Zen Blue |
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id | Lightpath |
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title | Lightpath |
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The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights. PDF |
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name | LightPath_Zeiss_Z1.pdf |
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Available manuals |
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| UI Expand |
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| - Check stage tilt
- Ask for Colibri Remote
- Ask for fluorescence backlight from LED
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| - Computer replacement
- Added Colibri
- Added startup procedure
- Parafocality and paracentrality
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| - Added complete description
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id | Technical Datasheet |
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title | Technical Datasheet |
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| StandLight sources- Transmitted LED light
- X-Cite 120Q Serial: TBD
CondenserObjectives2.5x/0.085 Air WD 8.8 - Empty
10x/0.25 Air Ph1 WD 6.5 - Empty
- 20x/0.5 Air Ph2 WD 2.0
- 40x/0.95 Air WD 0.25
Stage- Motorized stage Marzhauser Sensotech, Part number 432903-9011-000, #14 07 132052; 90-76-200-0820
- Remote control joystick
- Inserts
- Slide combo
- 6-well plate
- 35 mm dish
- Multi-well plat
Filters- DAPI Filter Set 49
- GFP Filter Set 13
- Rhodamine Filter Set 43
- DHE (dihydroethidium)
- Cy5 Filter Set 50
- Empty
Detector- Zeiss AxioCam MR R3 CCD Camera 1388 x 1040 pixels, 12-bit, 13 images/s at full resolution, detector size 8.9 mm x 6.7 mm. Model: r3.1 Part Number: 426509-9901-000. Serial: 1 22 12 5537
Workstation- Fujitsu Esprimo P920 E90+
- Intel Core i5-4670 @ 3.4 GHz
- RAM 32 GB DDR3 1600 MHz ECC (4 x 8 GB)
- OS 500 GB SSD 550 MB/s
- 2 TB HD Data Storage (2 x 1 TB spanned volume) 110 MB/s
- Video Card AMD FirePro V4900 1 GB DDR5 dedicated memory
- Monitor TBD display TBD' 1920 x 1200
- Software Zen Blue 2.0
IncubationConsumables- CO2 Tank
- N2 Tank
- Liquid Light Guide
- 100 W Mercury lamp
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id | FAQ |
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title | Troubleshooting & FAQ |
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| Troubleshooting UI Expand |
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title | I don't see any fluorescence! |
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| This microscope is motorized for most of its components, yet the X-Cite light source is manual. Ensure the X-Cite diaphragm (#4B) fully opened (high position). - On the X-Cite lamp
- Turn the intensity diaphragm (#4B) up
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FAQ UI Expand |
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title | Can I use this microscope to look at cell in a dish? |
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| - Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
- The objectives are optimized to image through thin glass bottom multi-well plates
- You may also image specimen mounted between a slide and a 0.17mm thick coverslip
- For long timelapse, be aware of photo-toxicity
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title | Can I use this microscope to perform timelapse experiments? |
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| Yes, but... This microscope has an incubation module to maintain temperature, humidity and gas. Yet it does not have a Definite focus which can maintain focus throughout time. Therefore, it is possible to loose the focus over long period. |
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