Empty

Tabs Container

id	Instrument Tabs
title	Zeiss Axio-Imager Z2
direction	horizontal

Tabs Page

id	Description
title	Description

Zeiss Axio-Observer spinning-disk microscope

J-A Bombardier Building, Room 3223-01
Advanced Microscope Tier 2 usage price

Instrument awarded to Dr. Daniel Zenklusen by the Canadian Foundation for Innovation (CFI)

Applications
- Transmitted light
- Interference Contrast (DIC)
- Fluorescence
Light sources
- 12V 100W halogen lamp for transmitted light
- Lumencor SOLA for visible fluorescence
  
  Développer
  title Lumencor SOLA complete specifications
  Emission peak (nm)
  Power (mW)
  334
  5
  365
  20
  405
  19
  436
  22
  546
  21
  579
  18
- 4 lasers 405, 488 561 638
  Développer
  title Laser complete specifications
  33419436225462157918
  Emission peak Laser line (nm)
  Nominal Power (mW)
  5
  365
  20
  405
  50
  488
  100
  561
  50
  639
  50

Objectives

10x100x/01.30 Air 46 Oil WD 5.300.11
TIRF Divergence adjusting aid
TIRF Angle adjusting aid
63x/1.

40

46 Oil WD 0.

19

1

100x

10x/

1.40

Oil

WD

0.

17

3 Air WD 5.2
TIRF adjustment
TIRF adjusment

Empty

Développer

title	Full lens specifications

Position

Nom

Marque

Nom complet

Identifiant

Grossissement

Ouverture numérique

Immersion

Type

Distance de travail (mm)

Transmittance

(% [nm])

Technique

Épaisseur du couvre-objet (mm)

1

10x

100x/

0.30
Air

1.46 Oil
Zeiss

10x

100x/

0

1.

3

46 DIC I

EC

Alpha Plan-

Neo Fluar

ApoChromat
M27

420340

420792-

9901-000

9800

10x

100x

0

1.

3

46
Remarque

Air

Oil
Plan

Neofluar

Apochromat

5

0.

2

11

>90%

>70% [

480

410-

780

800]
BF, DIC, Fluo
0.17
2

Empty

TIRF Divergence adjusting aid
Zeiss

20x/0.8 DIC II

Plan-Apochromat
W0.8x1/36"

440640-9903-000

20x

0.8

Air

Plan Apochromat

0.55

423682-8801-000

3

TIRF Angle adjusting aid

Zeiss

423682-8802-000

4

63x/1.46 Oil

Zeiss

63x/1.46 DIC III

Alpha Plan-Apochromat
Korr TIRF

M27

420780-9970-000

63x

1.46

Remarque
Huile

Plan Apochromat

0.10

>80% [500

>90% [410

-800]
BF, DIC, Fluo
0.15-0.

17

3

Zeiss

19
5	10x/0.3 Air	Zeiss	10x

40x

/0.

75 DIC II

3
EC Plan-

Neofluar

NeoFluar

M27

420360-9900-000

420340-9901
10x

40x

0.

75

3
Air
Plan

Neofluar

-NeoFluar

0

5.

71

2
>90% [

410

450-

780

750]
BF, DIC, Fluo
0.17

4

63x/1.40

Huile

Zeiss

63x/1.4 DIC III

Plan-Apochromat

M27

420782-9900-000

63x

1.4

Remarque
Huile

Plan Apochromat

0.19

>80% [440-710]

BF, DIC, Fluo

0.17

5

Empty

6

100x/1.40
Huile

Zeiss

100x/1.4 DIC III
Plan-Apochromat
M27

420792-9900-000

100x

1.4

Remarque
Huile

Plan Apochromat

0.17

>80% [400-820]

BF, DIC, Fluo

0.17

BF: Bright-field
DIC: Interference contrast

Filter cubes

DAPI

BP405

76

77

Empty
Développer

title	Complete filter specifications

Position

Nom

Marque

Identifiant

Filtre d'excitation

Miroir dichroïque

Filtre d'émission

Commentaire

1

DAPI

Filter Set 49

Zeiss

488049-9901-000

365/50

[340-390]

395LP

445/50

[420-470]

2

GFP

Filter Set 38

Zeiss

000000-1031-346

470/40
[450-490]

495LP

525/50

[500-550]

FT 495

BP 525/50

3

YFP

Filter Set 46

Zeiss

000000-1196-681

500/20

[490-510]

515LP

535/30

[520-550]

4

DsRed

Filter Set 43

Zeiss

000000-1114-101

545/25

[533-557]

570LP

605/70

[570-640]

FT 570

BP 605/70

5

TxRed

Filter Set 45

Zeiss

000000-1114-462

560/40

[540-580]

585LP

630/75

[593-667]

6

Cy3.0

Chroma

SP102v1

546/11
[541-551]

557LP

567/15

[560-574]

7

Cy3.5

Chroma

SP103v1

581/10
[576-586]

593LP

617/40

[597-637]

8

Cy5 narrow

Chroma

49009

640/30
[625-655]

660LP

690/50

[665-715]

9

Analyseur DIC

Zeiss

10

Vide

Detector
- 2 EMCCD camera Photometrics Evolve 512 x 512 pixels, 16-bit, 33 f/s at full resolution, sensor size 8.192 mm x 8.192mm, pixel size 16um x 16um
  Evolve512-Datasheet.pdf
  Evolve512_Manual.pdf

6
Empty

BF: Bright-field
DIC: Interference contrast

Filter cubes

Empty
BS_455
LSM TFT80/20 1447-381
BF RL TIRF Calibration 424928
Set 76 C/G/Dr
Set 77 G/R/A6

Développer

title	Complete filter specifications

Position	Nom	Marque	Identifiant	Filtre d'excitation	Miroir dichroïque	Filtre d'émission	Commentaire
1	Empty
2	BS_455	Zeiss			455LP		To be used with FRAP 405
3	LSM Duo T20/R80 UV	Zeiss
4	Brightfield Ref Light	Zeiss
5	Set 76 CFP GFP DsRed	Zeiss	489076-0000-000	390-422 484-501 549-573	427 503 578	448-472 512-538 585-631
6	Set 77 GFP/RFP/Alexa633	Zeiss	489077-0000-000	469-497 552-577 629-650	506 582 659	510-542 587-614 665-711

Detector
- 2 EMCCD camera Photometrics Evolve 512 x 512 pixels, 16-bit, 33 f/s at full resolution, sensor size 8.192 mm x 8.192mm, pixel size 16um x 16um
  Evolve512-Datasheet.pdf
  Evolve512_Manual.pdf

Tabs Page

id	User Guide
title	User Guide

UI Expand

expanded	true
title	Startup

Turn on the computer (#1)
If required, turn on the incubation power bar
Turn on the camera and laser power bar on the left of the microscope (#2)
Turn on the microscope power bar on the right of the microscope (#3)
Use your UdeM credentials to log in to Windows
Remarque
When using for the first time, it is necessary to import the microscope-specific parameters BEFORE starting the software. See the First Use section below.
Start the Zen Blue software

UI Expand

title	First Use

When using for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example.

Remarque
Please note, this procedure will delete all your experiment protocols and restore the software to its original settings.

If open, close the Zen Blue software and wait for it to close completely (up to 30 seconds)
On the Desktop open the Documentation folder
Double-click Settings for Axio-Observer Z1
A script will run and a black window will appear briefly
You can then reopen the Zen Blue software

UI Expand

title	Loading samples

This procedure puts the microscope in a safe configuration and performs a focus calibration. At the end of this procedure the microscope will be ready for acquisition.

UI Expand

title	Focus Calibration Z

On the microscope touch screen:

Press Home>Load Position to lower the stage to its lowest position
Press Set Work Position to store this position
If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
Press Home>Microscope>Turret>Objectives>10x to select the 10x objective
If asked, tap Done to remove the oil lens cleaning warning
Press Home>Microscope>XYZ>Position>Z-Position>Set zero>Auto to perform focus calibration
Press OK to start the focus calibration procedure
Wait a few seconds for the calibration to be completed

Remarque
Once calibrated, the focus can be found at Z = 5 mm). The Z value can be found on the microscope touch screen Home>Z-Position

UI Expand

title	First focus

Avertissement
Make sure to calibrate the focus before performing the first focus.

On the microscope touch screen:

Press Home>Microscope>Turret>Objectives

Press 10x to select the 10x lens

Info
The 10x objective is the safest because it has the longest working distance (5.3 mm). The sample will appear perfectly sharp long before the lens approaches it. It is recommended to always first focus with the safest lens. The objectives are para-focal, focusing with the safest objective will then allow you to easily find your sample with another objective.

Press Home>Load Position to lower the stage to its lowest position
Press Set Work Position to store this position
If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
Place the test slide on the microscope stage with the coverslip toward the objective
Remarque
Always use the test slide to perform the first focus.
If necessary, move the stage so that the sample is centered on the objective

On the computer:

Open the Zen Blue software
In the Locate tab, select BF or the desired fluorescence ((CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
Adjust the focus with the main dial while looking through the eyepieces until the image is perfectly sharp

Remarque
Once calibrated, the focus can be found at Z = 5 mm). The Z value can be found on the microscope touch screen Home>Z-Position
In the Locate tab, select Off to turn off the illumination

UI Expand

title	Seconday focus

Avertissement

title	Important

First focus with the safest lens before selecting another lens and continuing with secondary focus.

UI Expand

title	Focusing with air objectives

This microscope does not have additional air objectives. However, if there were, the procedure would be as follows:

After performing the first focus, on the microscope touch screen:

Press Home>Microscope>Turret>Objectives
Press on the desired lens

In Zen Blue software:

In the Locate tab, select BF or the desired fluorescence (CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
In the Locate tab, select Off to turn the illumination off
Your sample is ready for acquisition!

UI Expand

title	Focusing with oil lenses

After performing the first focus, on

Tabs Page

id	User Guide
title	User Guide

UI Expand

expanded	true
title	Startup

Turn on the computer (#1)
If required, turn on the incubation power bar
Turn on the camera and laser power bar on the left of the microscope (#2)
Turn on the microscope power bar on the right of the microscope (#3)
Use your UdM credentials to log in to Windows
Remarque
When using for the first time, it is necessary to import the microscope-specific parameters BEFORE starting the software. See the First Use section below.
Start the Zen Blue software

When using for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example.

UI Expand

title	First Use

Remarque
Please note, this procedure will delete all your experiment protocols and restore the software to its original settings.

If open, close the Zen Blue software and wait for it to close completely (up to 30 seconds)
On the Desktop open the Documentation folder
Double-click Settings for Axio-Observer Z1
A script will run and a black window will appear briefly
You can then reopen the Zen Blue software

UI Expand

title	Loading samples

This procedure puts the microscope in a safe configuration and performs a focus calibration. At the end of this procedure the microscope will be ready for acquisition.

UI Expand

title	Focus Calibration Z

On the microscope touch screen:

Press Home>Load Position to lower the stage to its lowest position
Press Set Work Position to store this position
If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
Press Home>Microscope>Turret>Objectives>10x to select the 10x objective
If asked, tap Done to remove the oil lens cleaning warning
Press Home>Microscope>XYZ>Position>Z-Position>Set zero>Auto to perform focus calibration
Press OK to start the focus calibration procedure
Wait a few seconds for the calibration to be completed

Remarque
Once calibrated, the focus can be found at Z = 5 mm). The Z value can be found on the microscope touch screen Home>Z-Position

UI Expand

title	First focus

Avertissement
Make sure to calibrate the focus before performing the first focus.

On

the microscope touch screen:

Press Home>Microscope>Turret>Objectives
Press

10x

63x Oil (1.4)or 100x Oil (1.4) to select the

10x lens

desired lens. The microscope will automatically lower the stage so that the sample is accessible.
Info
The

10x objective is the safest

63x objective is the best oil objective because it has the

longest working distance (5.3 mm). The sample will appear perfectly sharp long before the lens approaches it. It is recommended to always first focus with the safest lens. The objectives are para-focal, focusing with the safest objective will then allow you to easily find your sample with another objective.

Press Home>Load Position to lower the stage to its lowest position

Press Set Work Position to store this position

If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen

Place the test slide on the microscope stage with the coverslip toward the objective

Remarque
Always use the test slide to perform the first focus.

If necessary, move the stage so that the sample is centered on the objective

On the computer:

most optical corrections (Plan Apochromat) and the largest numerical aperture (1.4). It offers a lateral resolution of 240nm at a wavelength of 550nm.
Remove your sample from the microscope
Place a drop of oil on the objective
Replace your sample from the microscope
Press Done. The microscope will automatically return the sample to its original position

In Zen Blue software:

In the Locate tab, select BF or the desired fluorescence (CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
In the Locate tab, select Off to turn the illumination off
Your sample is ready for acquisition!

UI Expand

title	Storage management

Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

Remarque

In any case, your files should be removed from the C: drive.

Open the Zen Blue software

In the Locate tab, select BF or the desired fluorescence ((CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration

Adjust the focus with the main dial while looking through the eyepieces until the image is perfectly sharp

Remarque
Once calibrated, the focus can be found at Z = 5 mm). The Z value can be found on the microscope touch screen Home>Z-Position

In the Locate tab, select Off to turn off the illumination

UI Expand

title

Seconday focus

Avertissement

title	Important

First focus with the safest lens before selecting another lens and continuing with secondary focus.

UI Expand

title	Focusing with air objectives

This microscope does not have additional air objectives. However, if there were, the procedure would be as follows:

After performing the first focus, on the microscope touch screen:

Press Home>Microscope>Turret>Objectives
Press on the desired lens

In Zen Blue software:

In the Locate tab, select BF or the desired fluorescence (CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
In the Locate tab, select Off to turn the illumination off
Your sample is ready for acquisition!

UI Expand

title	Focusing with oil lenses

After performing the first focus, on the microscope touch screen:

Press Home>Microscope>Turret>Objectives

Press 63x Oil (1.4)or 100x Oil (1.4) to select the desired lens. The microscope will automatically lower the stage so that the sample is accessible.

Info
The 63x objective is the best oil objective because it has the most optical corrections (Plan Apochromat) and the largest numerical aperture (1.4). It offers a lateral resolution of 240nm at a wavelength of 550nm.

Remove your sample from the microscope

Place a drop of oil on the objective

Replace your sample from the microscope

Press Done. The microscope will automatically return the sample to its original position

In Zen Blue software:

In the Locate tab, select BF or the desired fluorescence (CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
In the Locate tab, select Off to turn the illumination off
Your sample is ready for acquisition!

UI Expand

title	Storage management

Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

Remarque
In any case, your files should be removed from the C: drive.

UI Expand

title	Shutdown

Save your data
Close the software Zen Blue
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
If used, turn of the incubation power bar
If used, clean oil lenses with lens cleaner and paper
Turn off the microscope power bar on the right of the microscope (#3)
Turn off the camera and laser power bar on the left of the microscope (#2)
Turn off the computer

Remarque

title	Important Reminders

Take back your samples including ones in the microscope
Leave the microscope and the working area clean

Tabs Page

id	Light Path
title	Light Path

The following diagrams allow you to follow the light path in transmitted light (bright field, DIC) and in reflected light (fluorescence).

Tabs Page

id	Manuals
title	Manuals

Zen quick start guide.pdf

Shutdown

Save your data
Close the software Zen Blue
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
If used, turn of the incubation power bar
If used, clean oil lenses with lens cleaner and paper
Turn off the microscope power bar on the right of the microscope (#3)
Turn off the camera and laser power bar on the left of the microscope (#2)
Turn off the computer

Remarque

title	Important Reminders

Take back your samples including ones in the microscope
Leave the microscope and the working area clean

Tabs Page

id	Light Path
title	Light Path

The following diagrams allow you to follow the light path in transmitted light (bright field, DIC) and in reflected light (fluorescence).

Tabs Page

id	Manuals
title	Manuals

Zen quick start guide.pdf

auitabspageidTechnical Datasheet

Tabs Page

id	Log
title	Log

UI Expand

title	To do

Check Loss of 488nm laser power

UI Expand

title	2024-04-26

(FIXED) Problem with the FRAP module:

At first could not modulate the laser intensity (yet could not find the spot on the field), rebooted and then the FRAP fiber does not carry any light (even 488)

==> Laser manipulation module was too far in x and y (noticeable as a tilt and a gap between both parts of the module).

UI Expand

expanded	true
title	2024-03-04

Corrected Trigger Module configuration for the BackCam

reassigned each camera with its own trigger module in MTB config

UI Expand

title	2024-02-29

DualCam calibration parameters set, using beads
Realigned Lasers:
- Laser output measured in spinning disk mode at the sample with 10x objective, 1x Extended tubelens, 100% laser power and ALL lasers ON:
  - 405nm: 0.70 mW before => 0.66 mW after
    - with laser 488 OFF: 0.81 mW before => 0.83 mW after
  - 488nm: 3.75 mW before => 4.17 mW after
    - with laser 405 OFF: 3.95 mW before => 4.33 mW after
  - 561nm: 2.1 mW before => 2.37 mW after
  - 639nm: 0.9 mW before => 1.1 mW after
NOTE: AOTF2 for FRAP

Remarque

title	AOTF2 calibration

For FRAP, the illumination is strongest with the AOTF2 at 60% and not 100%

Error message when switching between FRAP & SD illumination

Avertissement

title	AOTF2 Error message

FRAP/SD illumination switching triggers an error message " 'MTBAOTF2LaserLine6' is not supported by the current hardware"

Tabs Page

id	Log
title	Log

UI Expand

title	To do

Check Loss of 488nm laser power

UI Expand

title	2021-11-20

Zen 2.6 updated hotfix 12
Microsoft Windows update
Laser output measured in spinning disk mode at the sample with 10x objective, 1x Extended tubelens, 100% laser power and ALL lasers ON:
- 405nm: 1.08 mW StdDev 0.01mW
- 488nm: 3.3 mW StdDev <0.01mW (3.7 mW if laser 405 is OFF)
  Avertissement
  title 405nm and 488nm Interactions
  When both 405nm and 488nm laser are ON simultaneously, the output power is decreased by 11%.
- 561nm: 2.6 mW StdDev <0.01mW
- 639nm: 1.37 mW StDev 0.02 mW
Control for vibrations during construction work on the 4th floor

UI Expand

title	2021-11-03

Error when the definite focus is used (unexpected error the definite focus did not respond)
To solve it:
- Turn off the Zen Software
- Turn off the microscope power bar (#3)
- Turn off the definite focus by pressing and holding the definite focus button
- Turn on the definite focus by pressing once the definite focus button
- Wait until the definite focus display shows "Detecting stand"
- Turn on the microscope power bar (#3)
Confirm that the definite focus is showing in the microscope tactile display
Open Zen software and run an experiment using the definite focus to confirm it is fully functional

UI Expand

title	2021-09-27

Added to wiki

UI Expand

expanded

title
true

Technical Datasheet

2020-07-07

AOTF1 changed

Tabs Page

id	Technical Datasheet
title	Technical Datasheet

Stand

Zeiss AxioObserver Z1 upright System ID: 1023798490; Serial number: 3834004569
Left imaging port

Stand

Zeiss AxioObserver Z1 upright Serial:
Part Number:
System ID
Camera adapter Model 60N-C, 1", 1x, Model: 426114
Motorized Neutral density filters for transmitted light
Manual Field diaphragm for transmitted light
Manual polarizer

Left imaging port with manual splitter

camera adapter Model 60N-C,

1

2/3", 1x,

Model: 426114

Trinocular with 100% ocular 40% occular/70% camera and 100% manual splitter
3mm liquid light guide #805-0038
Zeiss 423302-0000 Collimator
Motorized Aperture diaphragm
Motorized Fluorescence field diaphragm

Light sources

Transmitted Halogen light 12V 100W HAL 100 #423000
- TBD Filters
Lumencor SOLA Serial

Condenser

Model: 426118-9000 for Yokogawa spinning disk
Yokogawa Spining disk CSU-X1 Serial 175116
Manual Field diaphragm for transmitted light
Right imaging port camera adapter witth slider 423685-9000 Axicam MRm r3.1 426509-9901-000 Serial 1-44-11-5127

Light sources

LED lamp for transmitted light 423053-9080
Lumencor SOLA V-nIR Serial 27536

Condenser

Motorized condenser NA 0.55 WD 26mm
Motorized condenser #424201-9902
Lens NA 0.9 WD TBD Part Number: TBD
Manual polarizer
Filter turret 6 positions manual
H

Empty

D Darkfield

DIC III #426706

Ph3

DICII #426702

Ph 2

DIC 426701

Empty
Empty
Empty
Empty
Empty

Ph 1

Objectives

10x/0.30 Air WD 5.30 DIC I Plan-NeoFluar M27 420340-9901-000
63x/1.40 46 Oil WD 0.19 DIC III Plan-Apochromat M27 420782-9900-00019
100x/1.40 46 Oil WD 0.17 DIC III Plan-Apochromat M27 420792-9900-000

Stage

Motorized stage Zeiss AIM System #2502000124
Remote control joystick
Inserts
- Slide only
- Plate

Filters

17

Stage

Motorized stage ASI MIV-2000
Remote control joystick ASI MS-2000 #1111-2112-3426-2020 Model WK-XYB-AV200-PZ
Remote touch screen 432907-9901
Inserts
- Combo Slide and petri ASI I-3091
- Plate ASI I-3020

Filters

610-positions motorized filter wheel #

DAPI Zeiss Filter Set 49 cube 424933

Detector

2 camera Evolve 512 Serial A10G104008 A16F104001
Definite Focus 1

Workstation

HP

Z800

Z840 Workstation Serial:

CZC1473Y0Q Part number: WJ112ECJ#AK6

CZC7498KCQ
2 x Intel Xeon

X5650 2.66

E5-2623 v3 3.0 GHz
RAM

24 GB DDR3 1333

64 GB DDR4 1200 MHz ECC (

12

4 x

2

16 GB)
OS

500 GB

1 TB SSD

410

550 MB/s

2

4 TB HD Data Storage (2 x

1

2 TB spanned volume) 110 MB/s
Video Card

ATI FirePro V5800 1

NVIDIA Quadro K2200 4 GB DDR5

Monitor Dell ST2410

Monitor HP ZR24W 24' 1920 x

1080

1200
Software Zen Blue 2.6 Hotfix 12

Incubation

Zeiss Incubation Pecon XL Multi S1 Dark LS Serial 0509311 Zeiss #411857-9540-000

Consumables

3mm liquid light guide THorlabsThorlabs
12V 100W halogen lamp OSRAM XenoPhot #64623 HLX

Tabs Page

id	FAQ
title	Troubleshooting & FAQ

Troubleshooting

UI Expand

title	I don't see my sample

The spinning disk is a multipoint confocal. Outside the focal plane the sample disappears completely. It is therefore easier to find your sample through the eyepieces. Follow the tuning procedure to achieve this.

UI Expand

title	I don't see any fluorescence!

The best way to solve a problem in Microscopy is to follow the light path. You will find in the Light path tab of this page, the diagrams which will allow you to follow the light all along its path through the microscope.

Open the light path file
Starting from the light source and moving towards the detector, verify that there is indeed light after each component of the microscope

FAQ

UI Expand

title	Can I use this microscope to look at cell in a dish?

Yes. It is an inverted microscope designed for the observation of living specimens. The spinning disk is particularly appreciated for its limited phototoxicity. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate. The objectives are optimized to image through thin glass bottom multi-well plates. You may also image specimen mounted between a slide and a 0.17mm thick coverslip.

Tabs Container

id	Demo Image
title	Zeiss Axio-Observer Z1 Spinning disk
direction	horizontal

Tabs Page

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Raccourcis espace

Arborescence des pages

Historique de la page

Comparaison des versions

Ancienne version 17

Nouvelle version Actuel

Légende

Zeiss Axio-Observer spinning-disk microscope

Stand

Stand

Light sources

Condenser

Light sources

Condenser

Objectives

Stage

Filters

Stage

Filters

Detector

Workstation

Incubation

Consumables

Troubleshooting

FAQ