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Button Hyperlink
iconbuild
titleFrançais
typeprimary
urlhttps://wiki.umontreal.ca/pages/viewpage.action?pageId=193498625

Tabs Container
idInstrument Tabs
titleZeiss Axio-Imager Z2
directionhorizontal
>90% [480-780]
Tabs Page
idDescription
titleDescription

Zeiss Axio-Observer spinning-disk microscope

J-A Bombardier Building, Room 3223-011 

Advanced Microscope Tier 2 usage price

Instrument awarded to Dr. Daniel Zenklusen by the Canadian Foundation for Innovation (CFI)

Applications

Info
titleMain applications
  • Transmitted light
  • Interference Contrast (DIC)
    • Fluorescence
    • Live-cell imaging
    • Incubator (Temperature & CO2)
    Fluorescence

    • Light sources

      • 12V 100W halogen lamp LED for transmitted light
      • Lumencor SOLA for visible fluorescence
        Développer
        titleLumencor SOLA complete specifications:

        Emission peak (nm)

        Power (mW)

        334

        5

        365

        20

        405

        19

        436

        22

        546

        21

        579

        18

      •  4 4x lasers: 405, 488 561 638
        Développer
        titleLaser complete specifications:
        Emission peak

        Laser line (nm)

        Nominal Power (mW)

        334

        5

        365

        20

        405

        19

        436

        22

        546

        21

        579

        18

    • Objectives

    10x/0.30 Air WD 5.30

      • Power at sample plane in mW

        (Obj. 10x; 2024/07/02)

        405

        50

        0.7

        488

        100

        3.3

        561

        50

        2.3

        639

        50

        1.1

    • Objectives

      1. 100x/1.46 Oil WD 0.11
      2. TIRF Divergence adjusting aid
      3. TIRF Angle adjusting aid 
      4. 63x/1.
    40
      1. 46 Oil WD 0.
    19
      1. 1
    100x
      1. 10x/
    1
      1. 0.
    40 Oil
      1. 3 Air WD
    0.17

  • TIRF adjustment

    • TIRF adjusment
      1. 5.2
      2. Empty
    Épaisseur du couvre-objet
    Développer
    titleFull lens specificationsObjectives Specifications

    Position

    Nom

    Name

    Marque

    Brand

    Nom complet

    Complete Name

    Identifiant

    Identifier

    Grossissement

    Magnification

    Ouverture numérique

    Numerical Aperture

    Immersion

    Type

    Distance de travail

    Working distance (mm)

    Transmittance
    (% [nm])

    Technique

    Techniques

    Coverslip thickness (mm)

    1

    10x

    100x/

    0

    1.

    30

    46 Oil

    Air

    Zeiss

    10x

    100x/

    0

    1.

    3 

    46 DIC I

    EC

    Alpha Plan-

    Neo Fluar

    ApoChromat
    M27

    420340

    420792-

    9901-000

    9800

    10x

    100x

    0

    1.

    3

    46

    Air

    Oil

    Plan

    Neofluar

    Apochromat

    5.2

    0.11


    Balise Wiki
    {+}>70% \[410-800\]+


    BF, DIC, Fluo

    0.17

    2

    Empty

    Zeiss

    20x/0.8 DIC II

    Plan-Apochromat
    W0.8x1/36"

    440640-9903-000

    20x

    0.8

    TIRF Divergence adjusting aid

    Zeiss


    423682-8801-000









    3

    TIRF Angle adjusting aid

    Zeiss


    423682-8802-000









    4

    63x/1.46 Oil

    Zeiss

    63x/1.46 DIC III
    Alpha Plan-Apochromat Korr TIRF
    M27

    420780-9970-000

    63x

    1.46

    Huile

    Air

    Plan Apochromat

    0.

    55

    10


    Balise Wiki
    {+}>80% \[500-800\]+

    >90% [410-800]


    BF, DIC, Fluo
    0.15-0.
    17
    3
    19
    5
    10x/0.3 Air
    Empty
    Zeiss
    40x
    10x/0.
    75 DIC II
    3  EC Plan-
    Neofluar
    NeoFluar
    M27
    420360-9900-000
    420340-9901
    10x
    40x
    0.
    75
    3
    Air
    Plan
     Neofluar
    -NeoFluar
    0.71
    >90% [410-780]
    5.2

     Balise Wiki
    {+}>90% \[450-750\]+

    BF, DIC, Fluo
    0.17
    4
    63x/1.40
    Huile
    Zeiss
    63x/1.4 DIC III
    Plan-Apochromat
    M27
    420782-9900-000
    63x
    1.4
     Remarque
    Huile
    Plan Apochromat
    0.19
    >80% [440-710]
    BF, DIC, Fluo
    0.17
    5
    Empty
    6
    100x/1.40
    Huile
    Zeiss
    100x/1.4 DIC III
    Plan-Apochromat
    M27
    420792-9900-000
    100x
    1.4
     Remarque
    Huile
    Plan Apochromat
    0.17
    >80% [400-820]
    BF, DIC, Fluo
    0.17
    BF: Bright-field 
    DIC: Interference contrast
    • Filter cubes
  • DAPI
  • BP405
  • 76
  • 77
    • Empty
     Développer
    titleComplete filter specifications
    Position
    Nom
    Marque
    Identifiant
    Filtre d'excitation 
    Miroir dichroïque
    Filtre d'émission
    Commentaire
    1
    DAPI
    Filter Set 49
    Zeiss
    488049-9901-000
    365/50
    [340-390]
    395LP
    445/50
    [420-470]
    2
    GFP
    Filter Set 38
    Zeiss
    000000-1031-346
    470/40
    [450-490]
    495LP
    525/50
    [500-550]
    FT 495
    BP 525/50
    3
    YFP
    Filter Set 46
    Zeiss
    000000-1196-681
    500/20
    [490-510]
    515LP
    535/30
    [520-550]
    4
    DsRed
    Filter Set 43
    Zeiss
    000000-1114-101
    545/25
    [533-557]
    570LP
    605/70
    [570-640]
    FT 570
    BP 605/70
    5
    TxRed
    Filter Set 45
    Zeiss
    000000-1114-462
    560/40
    [540-580]
    585LP
    630/75
    [593-667]
    6
    Cy3.0
    Chroma
    SP102v1
    546/11
    [541-551]
    557LP
    567/15
    [560-574]
    7
    Cy3.5
    Chroma
    SP103v1
    581/10
    [576-586]
    593LP
    617/40
    [597-637]
    8
    Cy5 narrow
    Chroma
    49009
    640/30
    [625-655]
    660LP
    690/50
    [665-715]
    9
    Analyseur DIC
    Zeiss
    10
    Vide
     Tabs Page
    idUser Guide
    titleUser Guide
     UI Expand
    expandedtrue
    titleStartup
    1. Turn on the computer (#1)
    2. If required, turn on the incubation power bar
    3. Turn on the camera and laser power bar on the left of the microscope (#2)
    4. Turn on the microscope power bar on the right of the microscope (#3)
    5. Use your UdM credentials to log in to Windows
       Remarque
      When using for the first time, it is necessary to import the microscope-specific parameters BEFORE starting the software. See the First Use section below.
    6. Start the Zen Blue software
     UI Expand
    titleFirst Use
    When using for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example.
     Remarque
    Please note, this procedure will delete all your experiment protocols and restore the software to its original settings.
    1. If open, close the Zen Blue software and wait for it to close completely (up to 30 seconds)
    2. On the Desktop open the Documentation folder
    3. Double-click Settings for Axio-Observer Z1
    4. A script will run and a black window will appear briefly
    5. You can then reopen the Zen Blue software
     UI Expand
    titleLoading samples
    This procedure puts the microscope in a safe configuration and performs a focus calibration. At the end of this procedure the microscope will be ready for acquisition.
     UI Expand
    titleFocus Calibration Z
    On the microscope touch screen:
    1. Press Home>Load Position to lower the stage to its lowest position
    2. Press Set Work Position to store this position
    3. If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
    4. Press Home>Microscope>Turret>Objectives>10x to select the 10x objective
    5. If asked, tap Done to remove the oil lens cleaning warning
    6. Press Home>Microscope>XYZ>Position>Z-Position>Set zero>Auto to perform focus calibration
    7. Press OK to start the focus calibration procedure
    8. Wait a few seconds for the calibration to be completed
     Remarque
    Once calibrated, the focus can be found at Z = 5 mm). The Z value can be found on the microscope touch screen Home>Z-Position
     UI Expand
    titleFirst focus
     Avertissement
    Make sure to calibrate the focus before performing the first focus.
    On the microscope touch screen:
    1. Press Home>Microscope>Turret>Objectives
    2. Press 10x to select the 10x lens
       Info
      The 10x objective is the safest because it has the longest working distance (5.3 mm). The sample will appear perfectly sharp long before the lens approaches it. It is recommended to always first focus with the safest lens. The objectives are para-focal, focusing with the safest objective will then allow you to easily find your sample with another objective.
    3. Press Home>Load Position to lower the stage to its lowest position
    4. Press Set Work Position to store this position
    5. If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
    6. Place the test slide on the microscope stage with the coverslip toward the objective
       Remarque
      Always use the test slide to perform the first focus.
    7. If necessary, move the stage so that the sample is centered on the objective
    On the computer:
  • Open the Zen Blue software
  • In the Locate tab, select BF or the desired fluorescence ((CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
    • Adjust the focus with the main dial while looking through the eyepieces until the image is perfectly sharp
     Remarque
    Once calibrated, the focus can be found at Z = 5 mm). The Z value can be found on the microscope touch screen Home>Z-Position
  • In the Locate tab, select Off to turn off the illumination
    •  UI Expand
    titleSeconday focus
     Avertissement
    titleImportant
    First focus with the safest lens before selecting another lens and continuing with secondary focus.
     UI Expand
    titleFocusing with air objectives
    This microscope does not have additional air objectives. However, if there were, the procedure would be as follows:
    After performing the first focus, on the microscope touch screen:
    1. Press Home>Microscope>Turret>Objectives
    2. Press on the desired lens
    In Zen Blue software:
    1. In the Locate tab, select BF or the desired fluorescence (CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
    2. Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
    3. In the Locate tab, select Off to turn the illumination off
    4. Your sample is ready for acquisition!
     UI Expand
    titleFocusing with oil lenses
    After performing the first focus, on the microscope touch screen:
  • Press Home>Microscope>Turret>Objectives
    • Press 63x Oil (1.4)or 100x Oil (1.4) to select the desired lens. The microscope will automatically lower the stage so that the sample is accessible.
     Info
    The 63x objective is the best oil objective because it has the most optical corrections (Plan Apochromat) and the largest numerical aperture (1.4). It offers a lateral resolution of 240nm at a wavelength of 550nm.
  • Remove your sample from the microscope
  • Place a drop of oil on the objective
  • Replace your sample from the microscope
  • Press Done. The microscope will automatically return the sample to its original position
    • In Zen Blue software:
    1. In the Locate tab, select BF or the desired fluorescence (CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
    2. Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
    3. In the Locate tab, select Off to turn the illumination off
    4. Your sample is ready for acquisition!
     UI Expand
    titleStorage management
    • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
    • At the end of each session, copy your data to your external drive and delete it from the local C: drive
    • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
     Remarque
    In any case, your files should be removed from the C: drive.
     UI Expand
    titleShutdown
    1. Save your data
    2. Close the software Zen Blue
    3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
    4. If used, turn of the incubation power bar
    5. If used, clean oil lenses with lens cleaner and paper
    6. Turn off the microscope power bar on the right of the microscope (#3)
    7. Turn off the camera and laser power bar on the left of the microscope (#2)
    8. Turn off the computer
     Remarque
    titleImportant Reminders
    • Take back your samples including ones in the microscope
    • Leave the microscope and the working area clean
     Tabs Page
    idLight Path
    titleLight Path
    The following diagrams allow you to follow the light path in transmitted light (bright field, DIC) and in reflected light (fluorescence).
     Tabs Page
    idManuals
    titleManuals
    6
    Empty











    BF: Bright-field 
    DIC: Interference contrast
    • Filter cubes:
      1. Empty
      2. BS_455
      3. LSM TFT80/20 1447-381
      4. BF RL TIRF Calibration 424928
      5. Set 76 C/G/Dr
      6. Set 77 G/R/A6
     Développer
    titleFilter sets - detailed properties
    PositionNomMarqueIdentifiantFiltre d'excitation Miroir dichroïqueFiltre d'émissionCommentaires
    1DAPI
    Filter Set 49    		Zeiss
    488049-9901
    		365/50
    [325-375]    		395LP445/50
    [420-470]

     Tabs Page
    idUser Guide
    titleUser Guide

     UI Expand
    expandedtrue
    titleStartup
    1. Turn on the computer (#1)
    2. If required, turn on the incubation power bar
    3. Turn on the camera and laser power bar on the left of the microscope (#2)
    4. Turn on the microscope power bar on the right of the microscope (#3)
    5. Use your UdeM credentials to log in to Windows
       Remarque
      When using for the first time, it is necessary to import the microscope-specific parameters BEFORE starting the software. See the First Use section below.
    6. Start the Zen Blue software

     UI Expand
    titleFirst Use
    When using for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example.
     Remarque
    Please note, this procedure will delete all your experiment protocols and restore the software to its original settings.
    1. If open, close the Zen Blue software and wait for it to close completely (up to 30 seconds)
    2. On the Desktop open the Documentation folder
    3. Double-click Settings for Axio-Observer Z1
    4. A script will run and a black window will appear briefly
    5. You can then reopen the Zen Blue software

     UI Expand
    titleLoading samples
    This procedure puts the microscope in a safe configuration and performs a focus calibration. At the end of this procedure the microscope will be ready for acquisition.
     UI Expand
    titleFocus Calibration Z
    On the microscope touch screen:
    1. Press Home>Load Position to lower the stage to its lowest position
    2. Press Set Work Position to store this position
    3. If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
    4. Press Home>Microscope>Turret>Objectives>10x to select the 10x objective
    5. If asked, tap Done to remove the oil lens cleaning warning
    6. Press Home>Microscope>XYZ>Position>Z-Position>Set zero>Auto to perform focus calibration
    7. Press OK to start the focus calibration procedure
    8. Wait a few seconds for the calibration to be completed
     Remarque
    Once calibrated, the focus can be found at Z = 5 mm). The Z value can be found on the microscope touch screen Home>Z-Position

     UI Expand
    titleFirst focus
     Avertissement
    Make sure to calibrate the focus before performing the first focus.
    On the microscope touch screen:
    1. Press Home>Microscope>Turret>Objectives
    2. Press 10x to select the 10x lens
       Info
      The 10x objective is the safest because it has the longest working distance (5.3 mm). The sample will appear perfectly sharp long before the lens approaches it. It is recommended to always first focus with the safest lens. The objectives are para-focal, focusing with the safest objective will then allow you to easily find your sample with another objective.
    3. Press Home>Load Position to lower the stage to its lowest position
    4. Press Set Work Position to store this position
    5. If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
    6. Place the test slide on the microscope stage with the coverslip toward the objective
       Remarque
      Always use the test slide to perform the first focus.
    7. If necessary, move the stage so that the sample is centered on the objective
    On the computer:
    1. Open the Zen Blue software
    2. In the Locate tab, select BF or the desired fluorescence ((CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
    3. Adjust the focus with the main dial while looking through the eyepieces until the image is perfectly sharp

       Remarque
      Once calibrated, the focus can be found at Z = 5 mm). The Z value can be found on the microscope touch screen Home>Z-Position

    4. In the Locate tab, select Off to turn off the illumination

     UI Expand
    titleSeconday focus
     Avertissement
    titleImportant
    First focus with the safest lens before selecting another lens and continuing with secondary focus.

     UI Expand
    titleFocusing with air objectives
    This microscope does not have additional air objectives. However, if there were, the procedure would be as follows:
    After performing the first focus, on the microscope touch screen:
    1. Press Home>Microscope>Turret>Objectives
    2. Press on the desired lens
    In Zen Blue software:
    1. In the Locate tab, select BF or the desired fluorescence (CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
    2. Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
    3. In the Locate tab, select Off to turn the illumination off
    4. Your sample is ready for acquisition!

     UI Expand
    titleFocusing with oil lenses
    After performing the first focus, on the microscope touch screen:
    1. Press Home>Microscope>Turret>Objectives
    2. Press 63x Oil (1.4)or 100x Oil (1.4) to select the desired lens. The microscope will automatically lower the stage so that the sample is accessible.
       Info
      The 63x objective is the best oil objective because it has the most optical corrections (Plan Apochromat) and the largest numerical aperture (1.4). It offers a lateral resolution of 240nm at a wavelength of 550nm.

    3. Remove your sample from the microscope
    4. Place a drop of oil on the objective
    5. Replace your sample from the microscope
    6. Press Done. The microscope will automatically return the sample to its original position
    In Zen Blue software:
    1. In the Locate tab, select BF or the desired fluorescence (CFP/GFP/DsRed ou GFP/RFP/Cy5, etc…) to activate the configuration
    2. Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
    3. In the Locate tab, select Off to turn the illumination off
    4. Your sample is ready for acquisition!
     UI Expand
    titleStorage management
    • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
    • At the end of each session, copy your data to your external drive and delete it from the local C: drive
    • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
     Remarque
    In any case, your files should be removed from the C: drive.
     UI Expand
    titleShutdown
    1. Save your data
    2. Close the software Zen Blue
    3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
    4. If used, turn of the incubation power bar
    5. If used, clean oil lenses with lens cleaner and paper
    6. Turn off the microscope power bar on the right of the microscope (#3)
    7. Turn off the camera and laser power bar on the left of the microscope (#2)
    8. Turn off the computer
     Remarque
    titleImportant Reminders
    • Take back your samples including ones in the microscope
    • Leave the microscope and the working area clean
     Tabs Page
    idLight Path
    titleLight Path

    The following diagrams allow you to follow the light path in transmitted light (bright field, DIC) and in reflected light (fluorescence).




     Tabs Page
    idManuals
    titleManuals


     Tabs Page
    idLog
    titleMaintenance Log
     UI Expand
    expandedtrue
    titleTo do list
    • 488nm laser power loss:  alignement
     UI Expand
    title2024-04-26
    (FIXED) Problem with the FRAP module:
    At first could not modulate the laser intensity (yet could not find the spot on the field), rebooted and then the FRAP fiber does not carry any light (even 488)
    ==> Laser manipulation module was too far in x and y (noticeable as a tilt and a gap between both parts of the module).
     UI Expand
    title2024-03-04
    Corrected Trigger Module configuration for the BackCam
    • reassigned each camera with its own trigger module in MTB config 
     UI Expand
    title2024-02-29
    • DualCam calibration parameters set, using beads
    • Realigned Lasers:
      • Laser output measured in spinning disk mode at the sample with 10x objective, 1x Extended tubelens, 100% laser power and ALL lasers ON:
        • 405nm: 0.70 mW before => 0.66 mW after
          • with laser 488 OFF: 0.81 mW before => 0.83 mW after
        • 488nm: 3.75 mW before => 4.17 mW after  
          • with laser 405 OFF: 3.95 mW before => 4.33 mW after
        • 561nm: 2.1 mW before => 2.37 mW after 
        • 639nm: 0.9 mW before => 1.1 mW after 
    • NOTE: AOTF2 for FRAP
    AOTF2 calibration
    For FRAP, the illumination is strongest with the AOTF2 at 60% and not 100%
    ·         Error message when switching between FRAP & SD illumination
    AOTF2 Error message
    FRAP/SD illumination switching triggers an error message " 'MTBAOTF2LaserLine6' is not supported by the current hardware"
     Tabs Page
    idLog
    titleLog
     UI Expand
    titleTo do
    Check Loss of 488nm laser power
    405nm and 488nm Interactions
    When both 405nm and 488nm laser are ON simultaneously, the output power is decreased by 11%.
      • 561nm: 2.6 mW StdDev <0.01mW
      • 639nm: 1.37 mW StDev 0.02 mW
    ·         Control for vibrations during construction work on the 4th floor
     UI Expand
    title2021-11-20
    • Zen 2.6 updated hotfix 12
    • Microsoft Windows update
    • Laser output measured in spinning disk mode at the sample with 10x objective, 1x Extended tubelens, 100% laser power and ALL lasers ON:
      • 405nm: 1.08 mW StdDev 0.01mW
    o    488nm: 3.3 mW  StdDev <0.01mW (3.7 mW if laser 405 is OFF)
    warning

    		title
     UI Expand
    title2021-11-03
    • Error when the definite focus is used (unexpected error the definite focus did not respond)
    • To solve it:
      • Turn off the Zen Software
      • Turn off the microscope power bar (#3)
      • Turn off the definite focus by pressing and holding the definite focus button
      • Turn on the definite focus by pressing once the definite focus button
      • Wait until the definite focus display shows "Detecting stand"
      • Turn on the microscope power bar (#3)
    • Confirm that the definite focus is showing in the microscope tactile display
    • Open Zen software and run an experiment using the definite focus to confirm it is fully functional
     UI Expand
    title2021-09-27
    Added to wiki
     UI Expand
    expandedtrue
    title2020-07-07
    • AOTF1 changed
     Tabs Page
    idTechnical Datasheet
    titleTechnical Datasheet
    Stand
    • Zeiss AxioObserver Z1 upright System ID: 1023798490; Serial number: 3834004569
    Stand
    • Zeiss AxioObserver Z1 upright Serial:
      Part Number: 
      System ID
    • Camera adapter Model 60N-C, 1", 1x, Model: 426114
    • Motorized Neutral density filters for transmitted light
    • Manual Field diaphragm for transmitted light
      • Manual polarizer
    • Left imaging port 
      •  with manual splitter 
    •  camera adapter Model 60N-C, 
      •  1
    • 2/3", 1x, Model: 
      •  426114
    • Trinocular with 100% ocular 40% occular/70% camera and 100% manual splitter
    • 3mm liquid light guide #805-0038
    • Zeiss 423302-0000 Collimator
    • Motorized Aperture diaphragm
    • Motorized Fluorescence field diaphragm
    Light sources
    • Transmitted Halogen light 12V 100W HAL 100 #423000
      • TBD Filters
    • Lumencor SOLA Serial 
    Condenser
    • 426118-9000 for Yokogawa spinning disk
    • Yokogawa Spining disk CSU-X1 Serial 175116

    • Manual Field diaphragm for transmitted light
    • Right imaging port camera adapter witth slider 423685-9000 Axicam MRm r3.1 426509-9901-000 Serial 1-44-11-5127
    Light sources
    • LED lamp for transmitted light 423053-9080
    • Lumencor SOLA V-nIR Serial 27536 
    Condenser
    • Motorized condenser NA 0.55 WD 26mm 
    • Motorized condenser #424201-9902
    • Lens NA 0.9 WD TBD Part Number: TBD
    • Manual polarizer
    • Filter turret 6 positions manual
      H 
    1. Empty
  • D Darkfield
  • DIC III #426706
  • Ph3
  • DICII #426702
  • Ph 2
  • DIC 426701
    1. Empty
    2. Empty
    3. Empty
    4. Empty
    5. Empty
    Ph 1
    Objectives
    1. 10x/0.30 Air WD 5.30 DIC I  Plan-NeoFluar M27 420340-9901-0005.30
    2. 63x/1.40 46 Oil WD 0.19 DIC III Plan-Apochromat M27 420782-9900-00019
    3. 100x/1.40 46 Oil WD 0.17 DIC III Plan-Apochromat M27 420792-9900-000 17 
    Stage
    • Motorized stage 
      •  Zeiss AIM System #2502000124
    • ASI MIV-2000
    • Remote control joystick ASI MS-2000 #1111-2112-3426-2020 Model WK-XYB-AV200-PZ
    • Remote touch screen 432907-9901
      • Remote control joystick
    • Inserts
      • Slide only
      • Combo Slide and petri ASI I-3091
      • Plate ASI I-3020
    Filters
    106-positions motorized filter wheel #
    DAPI Zeiss Filter Set 49 cube 424933
    Detector
    • 2 camera Evolve 512 Serial A10G104008 A16F104001
    • Definite Focus 1
    Workstation
    • HP 
      •  Z800 
    • Z840 Workstation Serial: 
      •  CZC1473Y0Q Part number: WJ112ECJ#AK6
    • CZC7498KCQ
    • 2 x Intel Xeon 
      •  X5650 2.66 
    •  E5-2623 v3 3.0 GHz
    • RAM 
      •  24 GB DDR3 1333 
    • 64 GB DDR4 1200 MHz ECC (
      • 12 
    • 4 x 
      •  2 
    • 16 GB)
    • OS 
      •  500 GB 
    • 1 TB SSD 
      •  410 
    • 550 MB/s
      • 2 
    • 4 TB HD Data Storage (2 x 
      •  1 
    • 2 TB spanned volume) 110 MB/s
    • Video Card 
      •  ATI FirePro V5800 1 
    • NVIDIA Quadro K2200 4 GB DDR5
      • Monitor Dell ST2410  
    • Monitor HP ZR24W  24' 1920 x 
      •  1080
    • 1200
    • Software Zen Blue 2.6 Hotfix 12
    Incubation
    • Zeiss Incubation Pecon XL Multi S1 Dark LS Serial 0509311 Zeiss #411857-9540-000
    Consumables

     Tabs Page
    idFAQ
    titleTroubleshooting & FAQ
    Troubleshooting
     UI Expand
    titleI don't see my sample
    The A spinning disk is a multipoint confocal.Outside is a multipoint confocal: due to the confocality, signal is restricted to the focal plane. Outside of the focal plane, the sample disappears completelyis not visible. It is therefore thus easier to find your sample through the eyepieces.Follow the tuning procedure to achieve thisthe proper focal plane using the oculars (wide-field signal). Please refer to first focus" procedure on the "loading samples" tab.
     UI Expand
    titleI don't see any fluorescence!
    The best way to solve a problem in Microscopy is to follow the light path. You will find in the Light path tab of this page, the diagrams which will allow you to follow the light all along its path through the microscope. 
    1. Open the light path file
    2. Starting from the light source and moving towards the detector, verify that there is indeed light after each component of the microscope
    FAQ
     UI Expand
    titleCan I use this microscope to look at cell in a dish?
    Yes. It is an inverted microscope designed for the observation of living specimens. The spinning disk is particularly appreciated for its limited phototoxicity and low out-of-focus background. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate. The objectives are optimized to image through thin glass bottom multi-well plates. You may also image specimen mounted between a slide and a 0.17mm thick coverslip.


     Tabs Container
    idDemo Image
    titleZeiss Axio-Observer Z1 Spinning disk
    directionhorizontal

     Tabs Page
    idDemo Image
    titleDemo Image




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