What is SEC-MALS?
Size Exclusion Chromatography with Multi-Angle Light Scattering analysis (SEC-MALS) is an absolute technique for the determination of the molar mass and the average radius of gyration (Rg) of biomolecules in solution following a separation by liquid chromatography. The technique uses static diffusion of a high intensity light source through a solution containing macromolecules. The intensity of this diffusion is then detected under several angles, hence being able to calculate precisely the molar mass and the average radius of gyration (Rg).
Tutorial sections
- Instrumentation:
- Overview of the instrumentation
- Software
- Available SEC columns
- SEC-MALS experiment
- Data analysis
References for this tutorial
Demonstration data
If you would like to explore this tutorial with demonstration data, you can download this datasets:
- BSA dataset (ZIP file) for the analysis of the molecular weight of a protein.
Websites
Software required
- ASTRA
Available SEC columns
The platform offers the following columns:
- Superdex 75, 3.2x300
- Superdex 200 Increase, 3.2x300
- Superdex 200 Increase, 10x300
- Superose 6 Increase, 3.2x300
- Superose 12, 3.2x300
Below is the fractionation range of difference SEC columns.
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