Tabs Container

id	Instrument Tabs
title	Zeiss Axio-Imager Z2
direction	horizontal

Cy3.0
Cy3.5

Detector

Photometrics Prime sCMOS monochrome camera 2048 x 2048 pixels, 16-bit, 30 fps at full resolution, 72% QE at 550nm
Prime-Datasheet.pdf
Prime-Manual.pdf

Tabs Page

id	Description
title	Description

Zeiss Axio-Imager Z2 upright microscope

J-A Bombardier Building, Room 3223-01
Advanced Microscope Tier 1 usage price

Instrument awarded to Dr. Pascal Chartrand by the Canadian Foundation for Innovation (CFI)

Applications
- Transmitted light
- Interference Contrast (DIC)
- Fluorescence
Light sources
- 12V 100W halogen lamp for transmitted light
- 200W Exact X-Cite Lamp (340~675nm) for Fluorescence

Full specifications of the exact

- Développer
  title Caractéristiques complètes de la lampe X-Cite

lamp

- exacte

Peak emission

- Emission peak (nm)
  Power (mW)
  334
  5
  365
  20
  405
  19
  436
  22
  546
  21
  579
  18
  X-Cite

Exact Quick Start Guide (English pdf)

- _exacte_User_Guide.pdf
  X-Cite

Exact User Guide (English pdf)
Comparison between HBO vs X-Cite Exact Mercury Lamp Emission Spectra (English) ( Source )

Objectives

- _exacte_Quick_Start_Guide.pdf
  Image Added

Objectives

10x/0

10x/0

.30 Air WD 5.30
20x/0.80 Air WD 0.61
40x/0.75 Air WD 0.71
63x/1.40

WD

Oil

0

WD 0.19
100x/1.

30

30 Oil WD 0.20

oil

100x/1.40 Oil WD 0.17

oil

Full lens specifications

BF: Bright-field
DIC: Interference contrast

Filter cubes

DAPI
GFP
YFP
DsRed/Cy3
TxRed
Cy3.0
Cy3.5
Cy5
DIC analyzer
Empty

Complete filter specifications

Position

Name

Brand

Identifier

Excitation filter

Dichroic mirror

Emission filter

Comment

1

DAPI
Filter Set 49

Zeiss

488049-9901-000

365/50
[ 340-390]

395LP

445/50
[420 -470]

2

GFP
Filter Set 38

Zeiss

000000-1031-346

470/40
[450 -490]

495LP

525/50
[500 -550]

FT 495

BP 525/50

3

YFP
Filter Set 46

Zeiss

000000-1196-681

500/20
[490 -510]

515LP

535/30
[520 -550]

4

DsRed
Filter Set 43

Zeiss

000000-1114-101

545/25
[533 -557]

570LP

605/70
[570 -640]

FT570

BP 605/70

5

TxRed
Filter Set 45

Zeiss

000000-1114-462

560/40
[540 -580]

585LP

630/75
[593 -667]

6

Chroma

SP102v1

546/11
[541 -551]

557LP

567/15
[560 -574]

7

Chroma

SP103v1

581/10
[576 -586]

593LP

617/40
[597 -637]

8

Cy5 narrow

Chroma

49009

640/30
[625 -655]

660LP

690/50
[665 -715]

9

DIC analyzer

Zeiss

10

Empty

Développer

title	Caractéristiques complètes des objectifs

Position

Nom

Marque

Nom complet

Identifiant

Grossissement

Ouverture numérique

Immersion

Type

Distance de travail (mm)

Transmittance

(% [nm])

Technique

Épaisseur du couvre-objet (mm)

1

10x/0.30
Air

Zeiss

10x/0.3 DIC I

EC Plan-Neo Fluar

M27

420340-9901-000

10x

0.3

Air

Plan Neofluar

5.2

>90% [480-780]

BF, DIC, Fluo

0.17

2

20x/0.80
Air

Zeiss

20x/0.8 DIC II

Plan-Apochromat
W0.8x1/36"

440640-9903-000

20x

0.8

Air

Plan Apochromat

0.55

>90% [410-800]

BF, DIC, Fluo

0.17

3

40x/0.75
Air

Zeiss

40x/0.75 DIC II
EC Plan-Neofluar
M27

420360-9900-000

40x

0.75

Air

Plan Neofluar

0.71

>90% [410-780]

BF, DIC, Fluo

0.17

4

63x/1.40

Huile

Zeiss

63x/1.4 DIC III

Plan-Apochromat

M27

420782-9900-000

63x

1.4

Remarque
Huile

Plan Apochromat

0.19

>80% [440-710]

BF, DIC, Fluo

0.17

5

100x/1.30
Huile

Zeiss

100x/1.3 DIC III
EC Plan-Neofluar
M27

420490-9900-000

100x

1.3

Remarque
Huile

Plan Neofluar

0.20

>80% [400-820]

BF, DIC, Fluo

0.17

6

100x/1.40
Huile

Zeiss

100x/1.4 DIC III
Plan-Apochromat
M27

420792-9900-000

100x

1.4

Remarque
Huile

Plan Apochromat

0.17

>80% [400-820]

BF, DIC, Fluo

0.17

BF: Bright-field
DIC: Interference contrast

Filter cubes

DAPI
GFP
YFP
DsRed/Cy3
TxRed
Cy3.0
Cy3.5
Cy5
DIC analyzer

Empty

Développer

title	Complete filter specifications

Position	Nom	Marque	Identifiant	Filtre d'excitation	Miroir dichroïque	Filtre d'émission	Commentaire
1	DAPI Filter Set 49	Zeiss	488049-9901-000	365/50 [340-390]	395LP	445/50 [420-470]
2	GFP Filter Set 38	Zeiss	000000-1031-346	470/40 [450-490]	495LP	525/50 [500-550]		FT 495	BP 525/50
3	YFP Filter Set 46	Zeiss	000000-1196-681	500/20 [490-510]	515LP	535/30 [520-550]
4	DsRed Filter Set 43	Zeiss	000000-1114-101	545/25 [533-557]	570LP	605/70 [570-640]	FT 570	BP 605/70
5	TxRed Filter Set 45	Zeiss	000000-1114-462	560/40 [540-580]	585LP	630/75 [593-667]
6	Cy3.0	Chroma	SP102v1	546/11 [541-551]	557LP	567/15 [560-574]
7	Cy3.5	Chroma	SP103v1	581/10 [576-586]	593LP	617/40 [597-637]
8	Cy5 narrow	Chroma	49009	640/30 [625-655]	660LP	690/50 [665-715]
9	Analyseur DIC	Zeiss
10	Vide

Detector
- Photometrics Prime sCMOS monochrome camera 2048 x 2048 pixels, 16-bit, 30 fps at full resolution, 72% QE at 550nm
  Prime-Datasheet.pdf
  Prime-Manual.pdf

When using for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example.

Tabs Page

id	User Guide
title	User Guide

UI Expand

expanded	true
title	Startup

Remove the dust cover from the microscope
Turn on the computer (#1)
Turn on the X-Cite exacte lamp on the shelf to the left of the microscope (#2)
Info
The X-Cite exact lamp display will flash during heating (approximately 4 minutes) and will then be operational.
Avertissement
The exact X-Cite lamp must be turned on for at least 30 minutes before being turned off and vice versa
Turn on the power bar on the shelf above the computer (#3)
Press the microscope start button located on the rear left of the microscope (#4)
Use your UdM credentials to log in to Windows
Remarque
When using for the first time, it is necessary to import the microscope-specific parameters BEFORE starting the software. See the First Use section below.
Start the Zen Blue software

UI Expand

title	First Use

When using for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example.

Remarque
Please note, this procedure will delete all your experiment protocols and restore the software to its original settings.

If open, close the Zen Blue software and wait for it to close completely (up to 30 seconds)
On the Desktop open the Documentation folder
Double-click Settings for Axio-Imager Z2
A script will run and a black window will appear briefly
You can then reopen the Zen Blue software

UI Expand

title	Loading samples

This procedure puts the microscope in a safe configuration and performs a focus calibration. At the end of this procedure the microscope will be ready for acquisition.

UI Expand

title	Focus Calibration Z

On the microscope touch screen:

Press Home>Load Position to lower the stage to its lowest position
Press Set Work Position to store this position
If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
Press Home>Microscope>Turret>Objectives>10x to select the 10x objective
If asked, tap Done to remove the oil lens cleaning warning
Press Home>Microscope>XYZ>Position>Z-Position>Set zero>Auto to perform focus calibration
Press OK to start the focus calibration procedure
Wait a few seconds for the calibration to be completed

Remarque
Once calibrated, the focus can be found at Z = 11 mm). The Z value can be found on the microscope touch screen Home>Z-Position

UI Expand

title	First focus

Avertissement

title	Important

Make sure to calibrate the focus before performing the first focus.

On the microscope touch screen:

Press Home>Microscope>Turret>Objectives

Press 10x to select the 10x lens

Info
The 10x objective is the safest because it has the longest working distance (5.3 mm). The sample will appear perfectly sharp long before the lens approaches it. It is recommended to always first focus with the safest lens. The objectives are para-focal, focusing with the safest objective will then allow you to easily find your sample with another objective.

Press Home>Load Position to lower the stage to its lowest position
Press Set Work Position to store this position
If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen
Place the test slide on the microscope stage with the coverslip toward the objective
Remarque
title Important
Always use the test slide to perform the first focus.
If necessary, move the stage so that the sample is centered on the objective

On the computer:

Open the Zen Blue software
In the Locate tab, select BF or the desired fluorescence (GFP, DsRed, DAPI, etc…) to activate the configuration
Adjust the focus with the main dial while looking through the eyepieces until the image is perfectly sharp

Remarque
Once calibrated, the focus can be found at Z = 11 mm). The Z value can be found on the microscope touch screen Home>Z-Position
In the Locate tab, select Off to turn off the illumination

UI Expand

title	Seconday focus

Avertissement

title	Important

First focus with the safest lens before selecting another lens and continuing with secondary focus.

UI Expand

title	Focusing with air objectives

After performing the first focus, on the microscope touch screen:

Press Home>Microscope>Turret>Objectives

Press 20x or 40x to select the desired lens

Info
The 20x objective is the best Air objective because it has the greatest number of optical corrections (Plan Apochromat) and the largest numerical aperture (0.8). It offers a lateral resolution of 420nm at a wavelength of 550nm.

Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
Your sample is ready for acquisition!

UI Expand

title	Focusing with oil lenses

After performing the first focus, on the microscope touch screen:

Press Home>Microscope>Turret>Objectives

Press 63x Oil, 100x Oil (1.3) ou 100x Oil (1.4) to select the desired lens. The microscope will automatically lower the stage so that the sample is accessible.

Info
The 63x objective is the best oil objective because it has the most optical corrections (Plan Apochromat) and the largest numerical aperture (1.4). It offers a lateral resolution of 240nm at a wavelength of 550nm.

Place a drop of oil on your sample
Press Done. The microscope will automatically return the sample to its original position

In Zen Blue software:

In the Locate tab, select BF or the desired fluorescence (GFP, DsRed, DAPI, etc…) to activate the configuration
Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
In the Locate tab, select Off to turn the illumination off
Your sample is ready for acquisition!

UI Expand

title	Storage management

Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

Remarque
In any case, your files should be removed from the C: drive.

UI Expand

title	Shutdown

Save your data
Close the software Zen Blue
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
If used, clean oil lenses with lens cleaner and paper
Turn off the power bar on the shelf above the computer (#3)
Turn off the X-Cite exacte lamp (#2)
Avertissement
The X-Cite lamp must be on for at least 30 min before being turned off and vice-versa
Turn off the computer
Cover the microscope

Remarque

title	Important Reminders

Take back your samples including ones in the microscope
Leave the microscope and the working area clean

Tabs Page

id	User Guide
title	User Guide

Remove the dust cover from the microscope

Turn on the computer (#1)

Turn on the exact X-Cite lamp on the shelf to the left of the microscope (#2)

UI Expand

expanded	true
title	Startup

Info
The X-Cite exact lamp display will flash during heating (approximately 4 minutes) and will then be operational.

Avertissement
The exact X-Cite lamp must be turned on for at least 30 minutes before being turned off and vice versa

Turn on the power bar on the shelf above the computer (#3)

Press the microscope start button located on the rear left of the microscope (#4)

Use your UdM credentials to log in to Windows

Remarque
Attention, cette procédure supprimera tous vos protocoles d’expérience et rétablira les paramètres originaux du logiciel.

Start the Zen Blue software

UI Expand

title	First Use

Remarque
Please note, this procedure will delete all your experiment protocols and restore the software to its original settings.

If open, close the Zen Blue software and wait for it to close completely (up to 30 seconds)

On the Desktop open the Documentation folderDouble-click Settings for Axio-Imager Z2

A script will run and a black window will appear briefly

You can then reopen the Zen Blue software

UI Expand

title	Loading samples

This procedure puts the microscope in a safe configuration and performs a focus calibration. At the end of this procedure the microscope will be ready for acquisition.

UI Expand

title	Focus Calibration

On the microscope touch screen:

Press Home>Load Position to lower the stage to its lowest position

Press Set Work Position to store this position

If necessary, move the focus slightly up to remove the “Lower Z limit reached” message displayed on the touchscreen

Press Home>Microscope>Turret>Objectives>10x to select the 10x objective

If asked, tap Done to remove the oil lens cleaning warning

Press Home>Microscope>XYZ>Position>Z-Position>Set zero>Auto to perform focus calibrationPress OK to start the focus calibration procedureWait a few seconds for the calibration to be completed

Remarque
Si calibrée, la mise au point est aux alentours de Z = 11 mm). La valeur de la position en Z est visible sur l'écran tactile Home>Z-Position

UI Expand

title	Première mise au point

Avertissement

title	Important

Faire d’abord la calibration de la mise au point avant d'effectuer la première mise au point.

Sur l’écran tactile du microscope :

Appuyez sur Home>Microscope>Turret>Objectives

Appuyez sur 10x pour sélectionner l'objectif 10x

Info

L’objectif 10x est le plus sécuritaire car il possède la plus grande distance de travail (5.3 mm). L'échantillon apparaitra parfaitement net bien avant que l'objectif ne s'approche de celui-ci. Il est recommandé de toujours faire la première mise au point avec l'objectif le plus sécuritaire. Les objectifs sont para-focaux, faire la mise au point avec l'objectif le plus sécuritaire vous permettra ensuite de trouver facilement votre échantillon avec un autre objectif.

Appuyez sur Home>Load Position pour faire descendre la platine dans sa position la plus basse

Appuyez sur Set Work Position pour mémoriser cette position

Si nécessaire, déplacez la mise au point légèrement vers le haut pour supprimer le message « Lower Z limit reached» qui s'affiche sur l'écran tactile

Placez la lame de test sur la platine du microscope avec le couvre-objet vers l'objectif

Remarque

title	Important

Toujours utiliser la lame de test pour effectuer la première mise au point.

Si nécessaire, déplacez la platine pour que l'échantillon soit centré sur l'objectif

Sur l’ordinateur :

Ouvrez le logiciel Zen Blue

Dans l’onglet Locate, sélectionnez BF ou la fluorescence désirée (GFP, DsRed, DAPI, etc…) pour activer la configuration

Ajustez la mise au point avec la molette principale tout en regardant dans les oculaires jusqu'à ce que l'image soit parfaitement nette

Remarque
Si calibrée, la mise au point est aux alentours de Z = 11 mm). La valeur de la position en Z est visible sur l'écran tactile Home>Z-Position

Dans l’onglet Locate, sélectionnez Off pour éteindre l'illumination

UI Expand

title	Mise au point secondaire

Avertissement

title	Important

Faire d’abord la première mise au point avec l'objectif le plus sécuritaire avant de sélectionner un autre objectif et de poursuivre avec la mise au point secondaire.

UI Expand

title	Mise au point avec les objectifs à air

Après avoir effectué la première mise au point, sur l’écran tactile du microscope :

Appuyez sur Home>Microscope>Turret>Objectives

Appuyez sur 20x ou 40x pour sélectionner l'objectif désiré

Info
L’objectif 20x est le meilleur objectif à Air car il possède le plus grand nombre de corrections optiques (Plan Apochromat) et la plus grande ouverture numérique (0.8). Il offre une résolution latérale de 420nm à une longueur d'onde de 550nm.

Ajustez la mise au point avec la molette de précision tout en regardant dans les oculaires jusqu'à ce que l'image soit parfaitement nette

Votre échantillon est prêt pour l’acquisition !

UI Expand

title	Mise au point avec les objectifs à l'huile

Après avoir effectué la première mise au point, sur l’écran tactile du microscope :

Appuyez sur Home>Microscope>Turret>Objectives

Appuyez sur 63x Oil, 100x Oil (1.4) ou 100x Oil (1.4) pour sélectionner l'objectif désiré. Le microscope abaissera automatiquement la platine pour que l'échantillon soit accessible.

Info
L'objectif 63x est le meilleur objectif à l'huile car il possède le plus grand nombre de corrections optiques (Plan Apochromat) et la plus grande ouverture numérique (1.4). Il offre une résolution latérale de 240nm à une longueur d'onde de 550nm.

Déposer une goutte d’huile sur votre échantillon

Appuyez sur Done. Le microscope replacera automatiquement l'échantillon à sa position originale.

Dans le logiciel Zen Blue :

Dans l’onglet Locate, sélectionnez BF ou la fluorescence désirée (GFP, DsRed, DAPI, etc…) pour activer la configuration
Ajustez la mise au point avec la molette de précision tout en regardant dans les oculaires jusqu'à ce que l'image soit parfaitement nette
Dans l’onglet Locate, sélectionnez Off pour éteindre l'illumination

Votre échantillon est prêt pour l’acquisition !

UI Expand

title	Gestion du stockage

Les fichiers peuvent être sauvés temporairement (pendant l’acquisition) sur le disque local C: (bureau)
À la fin de chaque session, copiez vos données sur votre disque externe et supprimez-les du disque local C:
Vous pouvez stocker vos fichiers sur le disque D: (Data Storage). Si vous utilisez ce disque, veuillez créer un dossier par laboratoire en utilisant le nom de famille du chercheur principal. À l’intérieur, créez un dossier par utilisateur (Prénom_Nom).

Remarque
Dans tous les cas, ne stockez pas vos fichiers sur le disque C:

UI Expand

title	Fermeture

Enregistrez vos données

Fermez le logiciel Zen Blue

Transférez vos données sur le disque D: (Data Storage) ou sur votre disque dur externe et les supprimer du disque local C:

Éteindre l'ordinateur

Éteindre la barre d'alimentation sur l'étagère au dessus de l'ordinateur (#3)

Éteindre la lampe X-Cite exacte (#2)

Avertissement
La lampe X-Cite exacte doit être allumée pendant au moins 30 minutes avant d’être éteinte et vice-versa

Couvrir l’instrument avec la housse de protection

Remarque

title	Rappels Importants

Récupérez vos échantillons notamment ceux dans le microscope
Laissez le microscope et l’espace de travail propre

Tabs Page

id	Light Path
title	Light Path

The following diagrams allow you to follow the light path in transmitted light (bright field, DIC) and in reflected light (fluorescence).

View file

name

Zeiss_Z2_Lightpath.pdf

height

250

Tabs Page

id	Manuals
title	Manuals

	Zeiss_Z2_Lightpath.pdf
height	250

Tabs Page

id	Manuals
title	Manuals

Tabs Page

id	Log
title	Log

UI Expand

title	To do

Quality control for Illumination, Liquid light guide and filters quality.
Camera sensor clean up

UI Expand

title	2024-06-05

Cy5 channel was misaligned with TXR & Cy3: switched Cy5 & TXR fllter cubes positions (7 ↔ 8). Cy5 (blue signal) was shifted in y relative to TXR and AF555 channels (left), and appears to colocalize after cube slot switch (right):

Image Added Image Added

Please advise platform managers if other channels appear misaligned.

UI Expand

title	2024-02-14 Added to wiki

User guide added to Wiki French and english version

Tabs Page

id	Log
title	Log

UI Expand

title	To do

Quality control for Illumination, Liquid light guide and filters quality.
Camera sensor clean up

UI Expand

title	2022-03-28

Objective 10-0.3 Plan NeoFluor #420340-9901-000 added
Parafocality adjustment using 100x-1.4 as reference
Condenser removed and cleaned (broken glass)

UI Expand

title	2021-10-20

Zen 2.6 updated hotfix 12
Microsoft Windows updated

UI Expand

title	2021-10-18

X-Cite Liquid light guide replaced (Transmittance was 75% remaining)
Fluorescence Light bulb replaced (was 2593 hours)
Power output at the sample using 20x objective GFP cube 488nm is 116mW
Objective parafocality adjusted
Objective Focus speed was changed 10x: 7; 20x: 6; 4-x: 5; 63x: 3; 100x-1.3: 3; 100x-1.4: 3.
Condenser lens cleaned (oil)
Data storage OK

UI Expand

title	2021-09-27

Added to wiki

Tabs Page

id	Technical Datasheet
title	Technical Datasheet

Stand

Zeiss AxioImager Z2 upright Serial: 35230001000
Part Number: 430000-9902
System ID 1022265893
Camera adapter Model 60N-C, 1", 1x, Model: 426114
Motorized Neutral density filters for transmitted light
Manual Field diaphragm for transmitted light
Manual polarizer
Left imaging port with manual splitter camera adapter Model 60N-C, 1", 1x, Model: 426114
Trinocular with 100% ocular 40% occular/70% camera and 100% manual splitter
3mm liquid light guide #805-0038
Zeiss 423302-0000 Collimator
Motorized Aperture diaphragm
Motorized Fluorescence field diaphragm

Light sources

Transmitted Halogen light 12V 100W HAL 100 #423000
- TBD Filters
X-Cite exacte 200 W Model XCT10A Serial: XCT10A-0156

Condenser

Motorized condenser #424201-9902
Lens NA 0.9 WD TBD Part Number: TBD
Manual polarizer
Filter turret 6 positions manual
1. H Empty
2. D Darkfield
3. DIC III #426706
4. Ph3
5. DICII #426702
6. Ph 2
7. DIC 426701
8. Ph 1

Objectives

10x/0.30 Air WD 5.30 DIC I Plan-NeoFluar M27 420340-9901-000
20x/0.80 Air WD 0.61 DIC II Plan-Apochromat W0.8x1/36" 440640-9903-000
40x/0.75 Air WD 0.71 DIC II EC Plan-NeoFluar M27 420360-9900-000
63x/1.40 Oil WD 0.19 DIC III Plan-Apochromat M27 420782-9900-000
100x/1.30 Oil WD 0.20 DIC III Plan-NeoFluar M27 420490-9900-000
100x/1.40 Oil WD 0.17 DIC III Plan-Apochromat M27 420792-9900-000

Stage

Motorized stage Zeiss AIM System #2502000124
Remote control joystick
Inserts
- Slide only

Filters

10-positions motorized filter wheel #424913 #424905-0160-810

DAPI Zeiss Filter Set 49 cube 424933
GFP Zeiss Filter Set 38 cube 424933
YFP Zeiss Filter Set 46 cube 424933
DsRed Zeiss Filter Set 43 cube 424933
TxRed Zeiss Filter Set 45 cube 424933
Cy3.0 Chroma #SP102v1 C162410
Cy3.5 Chroma #SP103v1 C162411
Cy5 Chroma #49009 C162412
DIC Analyzer Zeiss 424932-01
Empty

Detector

Photometrics Prime Camera sCMOS 2048 x 2048 pixels, 16-bit, 30 images/s at full resolution, detector size 13.312mm x 13.312mm (18.8 mm diagonal). Serial: A18B202001. Cameralink cable and PCIe adapter available for imaging 100 images/s

Workstation

HP Z800 Workstation Serial: CZC1473Y0Q Part number: WJ112ECJ#AK6
2 x Intel Xeon X5650 2.66 GHz
RAM 24 GB DDR3 1333 MHz ECC (12 x 2 GB)
OS 500 GB SSD 410 MB/s
2 TB HD Data Storage (2 x 1 TB spanned volume) 110 MB/s
Video Card ATI FirePro V5800 1 GB DDR5
Monitor Dell ST2410 24' 1920 x 1080
Software Zen Blue 2.6 Hotfix 12

Incubation

None

Consumables

3mm liquid light guide X-Cite #805-0038
X-Cite exacte 200 W replacement bulb X-Cite Lumen Dynamics Part number 012-66000R
12V 100W halogen lamp OSRAM XenoPhot #64623 HLX

Tabs Page

id	FAQ
title	Troubleshooting & FAQ

Troubleshooting

UI Expand

title	I don't see any fluorescence!

The best way to solve a problem in Microscopy is to follow the light path. You will find in the Light path tab of this page, the diagrams which will allow you to follow the light all along its path through the microscope.

Open the light path file
Starting from the light source and moving towards the detector, verify that there is indeed light after each component of the microscope

FAQ

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title	Can I use this microscope to observe cells in culture?

No. It is an upright microscope designed for the observation of specimens between a slide and a coverslip (thickness 0.17mm).

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Zeiss Axio-Imager Z2 upright microscope

Stand

Light sources

Condenser

Objectives

Stage

Filters

Detector

Workstation

Incubation

Consumables

Troubleshooting

FAQ

Raccourcis espace

Arborescence des pages

Historique de la page

Comparaison des versions

Ancienne version 21

Nouvelle version Actuel

Légende

Zeiss Axio-Imager Z2 upright microscope

Stand

Light sources

Condenser

Objectives

Stage

Filters

Detector

Workstation

Incubation

Consumables

Troubleshooting

FAQ