Tabs Container

id	Instrument Tabs
title	Olympus BX63
direction	horizontal

TBD

Tabs Page

id	Description
title	Description

Olympus BX63 upright microscope

P-G Desmarais Building, Room 5308-2
Advanced Microscope Tier 1 usage price

Instrument awarded to Dr Marina Martinez by the Canadian Foundation for Innovation (CFI)
Advanced Microscope Tier 1 usage price

Applications
- Transmitted light, Bright-field
- Phase contrast
- Polarized light, DIC
- Fluorescence
- Color camera
Light sources
- LED lamp for transmitted light
- X-Cite NOVEM-S XT910 for fluorescence

Développer

title	X-Cite NOVEM XT910 complete specifications

Source	Excitation wavelengths (nm)	Compatible fluorophores
365		DAPI, Hoechst
435		CFP
475		FITC, GFP
500-600	- [500-600]
	509/22 [498-520]	YFP
	554/23 [542-565]	TRITC
	578/21 [567-588]	mCherry
635		Cy5
735		Cy7

Objectives
1. 4x/0.16 Air WD TBD16
2. 10x/0.4 Air WD TBD3.1
3. 20x/0.75 Air WD TBD0.6
4. 40x/0.95 Air WD TBD0.18
5. 60x/1.35 Oil WD TBD0.15
6. 100x/1.4 Oil WD TBD0.13
7. Empty

Développer

title	Objectives complete specifications

Développer

title	Caractéristiques complètes des objectifs

Position	Nom	Marque	Nom complet	Identifiant	Ouverture numérique	Immersion	Distance de travail (mm)	Transmittance (% [nm])	Technique	Épaisseur du couvre-objet (mm)
1	4x/0.16 Air	Olympus	4x/0.16 Air UPlanSApo	UPLXAPO4X.pdf	0.16	Air

TBD

16	>90% at 550nm Image Added	BF, Fluo	-
2	10x/0.4 Air	Olympus	10x/0.4 Air UPlanSApo	UPLXAPO10X.pdf	0.4	Air

TBD

3.1

~90% at 550nm

Image Added

BF, Pol, DIC, Fluo

TBD

	0.17
3	20x/0.75	Olympus	20x/0.75 Air UPlanSApo	UPLXAPO20X.pdf	0.75	Air

TBDTBD

0.6

>90% at 550nm

Image Added

BF, Pol, DIC, Fluo

TBD

	0.17
4	40x/0.95 Air	Olympus	40x/0.95 Air UPlanSApo

0

UPLXAPO40X.pdf

0.95

Air

TBDTBDTBDAjustable

	0.18	~90% at 550nm Image Added	BF, Pol, DIC, Fluo	Adjustable 0.11-0.23
5	60x/1.35

Huile

Oil

Olympus

60x/1.35 Oil
UPlanSApo

UPLXAPO60XO.pdf

1.35

Remarque
Oil

Huile

0.15

~90% at 550nm

Image Added

BF, Pol, DIC, Fluo

TBDTBDTBD

	0.17
6	100x/1.4

Huile

Oil

Olympus

100x/1.4 Oil
UPlanSApo

UPLXAPO100XO.pdf

1.4

Remarque

HuileTBDTBD

Oil

0.13

~90% at 550nm

Image Added

BF, Pol, DIC, Fluo

TBD

	0.17
7	Vide

BF:

Champ clair (

Bright-field

)

Pol:

Lumière polarisée
PhC: Contraste de phase

Polarized light
DIC: Differential interference contrast
Fluo: Fluorescence

Filters
1. Empty
Filters
2. DAPI
3. GFP/FITC (CFP)
4. Cy5
5. DAPI/GFP/Cy3/Cy5 (requires Cy3 filter in Lumencor SpectraX Green/Yellow position)
6. DIC Analyzer
7. FITC
8. TRITC
9. mCherry
10. Empty
11. Empty
12. U-FDICT DIC Analyzer (Nomarski prismCFP/YFP/mCherry (requires mCherry filter in Lumencor SpectraX Green/Yellow position)

Développer

title	Filters complete specifications

525/50m
[500-550]

Position	Name	Brand	ID	Excitation Filter	Dichroic mirror	Emission Filter	Comments
1	DAPIEmpty
Nikon2	DAPI-U HQ	395/25x [383-408]	425LP	460/50m [435-485]	C-FL-C DAPI-U HQ	2	GFP	Semrock	GFP-4050B-000	466/40x [446-486]	495LP	Nikon ID 96372
3	Cy5	Semrock	Cy5-5070A	617/55x [590-645]	652LP	697/77m [659-736]	Nikon ID 96376
4	DAPI/GFP/Cy3/Cy5	Semrock	C182279	None	409/493/573/652	432/515/595/681	77074160 Custom Quad C182279. Excitation filters are in the Lumencor SpectraX light source
5	DIC Analyzer	Nikon	Ti2-C-DICACL	Not Applicable	Not Applicable	Not Applicable	Used for DIC imaging
6	CFP\YFP\mCherry	Semrock	C1997767	None	459/526/596	475/543/702	Excitation filters are in the Lumencor SpectraX light source
	Olympus	U-FUNA	360-370	410LP	420-460	Image Added
3	FITC	Olympus	U-FBNA	470-495	505LP	510-550	Image Added
4	TRITC	Olympus	U-FGWA	530-550	570LP	575-625	Image Added
5	mCherry	Olympus	U-FYW	540-585	595LP	600LP	Image Added
6	Empty
7	Empty
8	DIC Analyzer	Olympus	U-FDICT DIC	-	-	-	DIC Analyzer

DetectorDetector
- Olympus DP80 Dual sensors (color and monochrome) CCD camera, 6.45 x 6.45 um pixel size,15 images/s at full frame 55% QE at 550nm
  Color mode 4080 x 3072 pixels, 24-bit
  Monochrome mode 1360 x 1024 pixels, 14-bit
  Olympus_DP80 Camera_User Guide.pdf

When using the microscope for the first time, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

Tabs Page

id	User Guide
title	User Guide

UI Expand

expanded	true
title	Startup

Turn on the computer (#1)
Remove the cover Remove the dust cover from the microscope
Turn on the computer (#1)
Turn on the microscope power bar(#2)
If incubation is required, turn Turn on the Okolab incubation module (#3A), the Lauda water bath (#3B) and open the CO₂ (#3C) and N₂ (#3D) tanks
Avertissement
Make sure the humidifier and the water bath are clean and properly filled with distilled water.
microscope touchscreen (switch is located at the back on the right of the touch screen) (#3)
Use your UdeM credentials to log in to Windows
Start the software NIS-Elements
Info
The first time you use the instrument, you need to import the microscope settings into the software. To do this follow the instructions in the First use

protocol

section below.
Start the software CellSens Dimension

Collect your samples, especially those in the microscope

Leave the microscope and workspace clean

UI Expand

title	Shutdown	First use

When using the microscope for the first time, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

Remarque
This process will delete all experiment protocols and reset the software to the original settings for this specific microscope.

If open, close the software CellSens Dimension and wait until it is completely closed (up to 30 seconds)
On your Desktop open the folder Documentation
Double-click Settings for BX-63
A script will run and a black window will briefly appear
You can now reopen the software CellSens Dimension

Save your data

Close NIS-Elements software

Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive

Turn off the computer

If oil objectives were used, clean it with lens cleaner and lens paper (not Kimwipes)

If incubation was used, turn off the Okolab incubation module (#3A),Lauda water bath (#3B) and close the CO₂ (#3C) and N₂ (#3D) tanks

Wait until the SpectaX has cooled down and turn off the microscope power bar (#2)

Cover the microscope

Remarque

title	Important Reminders

Files can be saved temporarily (during acquisition) to local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create one folder per laboratory using the principal investigator's last name. Inside, create a folder per user using the following nomenclature (First Name_Last Name).

UI Expand

title	Storage management

Remarque

title	Important

In any case, do not store your files on the C: drive.

UI Expand

title	First use

Ancre

FirstUse

Remarque
This process will delete all experiment protocols and reset the software to the original settings for this specific microscope.

If open, close NIS-Elements and wait until it is completely closed (up to 30 seconds)
On your Desktop open the Softwares folder
Open NIS Settings Utility
Click on the Import tab
Click on Browse
Navigate to your Desktop
Select the file Nikon-Ti2 Settings.bin
Click Select
Select all items
Click Import
Click OK
Close the NIS Settings
You can now reopen NIS-Elements

Tabs Page

id	Light path
title	Light path

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.

PDF

name	LightPath_Nikon Ti2.pdf

Tabs Page

id	Manuals
title	Manuals

Available manuals

Tabs Page

id	Log
title	Log

UI Expand

expanded	true
title	To do

Check condenser filters if labels match
Check why 20x/0.75 DIC objective has no DIC prism
Condenser DIC prism is DIC N1 while objective requires N2
Check why at 4x Condenser gives a black shadow on the righ side of the image
Adjust Objective XYZ offset
Check plane because FOV is not flat

UI Expand

expanded	true
title	2023-11-29

Fixed condenser holder loose
20x/0.5 Ph1 dirty or damaged likely dirty with varnished

UI Expand

title	2022-09-22

Added network cable between Acquisition and Processing workstations
Added shortcuts in Desktop\Documentation

UI Expand

title	2022-03-16

Clean 60x objective (nail polish on it !)
Fix damaged stage insert
Stage insert tilt adjustment
Objective XY offset adjustments

UI Expand

title	2022-03-16

Installed new objective 4x/0.2 Plan Apo Lambda
Updated configuration file

UI Expand

title	2022-02-21

Computer maintenance
Imaging test slides
Re-install DCAM API drivers v22.2.6391
Okolab incubation complete maintenance
- Disassemble Okolab stage insert
- Disconnect the pipes
- Disconnect the Lauda water-bath
- Clean everything with 10% acetic acid (excepted the plastic pipes and washers)
- Rinse with regular tap water 2 times
- Rinse with distilled water once
- Dry, reassemble and reconnect
- Refill with the Lauda water-bath with 3L of milliQ water
- Set the Okolab temperature Control Mode to Chamber
- Set the Okolab temperature to 65°C
- Let run for 1h. This will sterilize the water.
- Check for any leakage
- Set the Okolab temperature Control Mode back to Sample
- Set the Okolab temperature back to 37°C

UI Expand

title	2021-12-16

Cleaning objectives
Control for incubation chamber liquid intrusion

UI Expand

title	2021-11-30

CO₂ Calibration for Okolab: Offset of 1%

UI Expand

title	2021-11-05

Control for illumination quality
See Illumination Report_Nikon Ti2_2021-11-05.pdf
Liquid light guide adjustment

UI Expand

title	2021-09-16

20x/0.75 DIC objective was dirty and has been cleaned
Printed and displayed a Memo about available air and oil objectives
Adjustment of camera angle
Objective calibration
Objective XY offset
Updated NIS settings
Added light path schematics to wikipedia

UI Expand

title	2021-09-15

Okolab display a NAN message for the free thermal sensor
The probe is made of two wires that need to be in contact to measure the temperature properly
- Turn off the Okolab module
- Disconnect the free sensor probe
- Cut out the damaged part
- Carefully expose the two wires
- Connect the two wires together

UI Expand

title	2021-09-13

Added label on gas bottles
Added line mark on humidifier

UI Expand

title	2021-07-20

512 GB SSD installed for OS
Windows 10 Installation
BIOS updated to v2.47

Tabs Page

id	Technical Datasheet
title	Technical Datasheet

Stand

Nikon Ti2-E inverted Serial 540156 System 170110-Sys-006287

Light sources

Transmitted LED light
- ND32 filter
- IR filter
- Manual Polarizer
Lumencor SpectraX 6-NII-SE Serial 9409

Condenser

Motorized condenser Ti2-C-TC-E Serial 519097
Lens LWD NA 0.52
Filter turret 7 motorized positions
1. Empty
2. Empty
3. Ph1
4. Ph3
5. Shutter
6. Empty
7. DIC N1

Objectives

20x/0.5 Air Ph1 WD 2.1
60x/1.4 Oil DIC WD 0.13
100x/1.45 Oil Ph3 WD 0.13
100x/1.45 Oil DIC WD 0.13
4x/0.2 Air WD 20
20x/0.75 Air DIC WD 1.0

Stage

Motorized stage Ti2 SHU compatible Serial 127808
Remote control joystick Ti2-S-JS Serial 127976
Inserts
- Multi-well plate Ti2-S-HW with tilt adjustment (no incubation)
- Combo slide 3cm dish Ti2-S-HU with tilt adjustment (no incubation)
- Okolab H101-CellASIC Frame with perfusion ports
  - 1 x 35mm petri dish 1x35-M + cover
  - 2 Chamber Slide 2xGS-M+ cover
  - 1 Multi-well for oil objectives MW-OIL
  - 6-well plate 6MW+ cover
  - 1 CellASIC + cover

Filters

DAPI Cube Ex 383-408 DAPI-U DM 425 BA 435-485
GFP Cube Semrock 96372 M349727 17
Cy5 Cube Semrock 96376 M351081 8
77074160 Custom Quad C182279 Polychroic and quad bandpass emitter for use with the following single bandpass filters: ET395/25x, ET470/24x, ET550/15x, ET640/30x
DIC Analyser Ti2-C-DICACL
C197767 7707\4656 CFP\YFP\mCherry XT

Detector

Hamamatsu ORCA Flash V2 C11440-22CU CMOS Monochrome Camera 2048 x 2048 pixels, 16-bit, 30fps at full resolution Serial 101081

Workstation

HP Z440 Workstation
Intel Xeon E5-1620 v4 @ 3.5GHz
RAM 32 GB DDR4 2400 MHz ECC (4 x 8 GB)
OS 500 GB SSD 530 MB/s
4 TB HD Data Storage (2 x 2 TB spanned volume) 130 MB/s
Video Card nVidia GTX 1080 8GB DDR5 dedicated memory
Monitor HP Z24i display 24' 1920x1200
Software NIS-Elements AR v5.02

Incubation

Okolab BoldLine Temperature unit Serial 284-1058 H101 T Unit BL
Okolab BoldLine CO₂/O₂ Unit 0-10/1-18 Serial 088-1102
Okolab OkoTouch Serial 118-224
Lauda water-bath Model Eco RE415 S LCK 4910 Serial LCK-4910-16-0006 Okolab 1322-1006 2017-05-30

Consumables

CO₂ Tank
N₂ Tank
Liquid Light Guide

Loading sample and focusing

Cette procédure permet de mettre le microscope dans une configuration sécuritaire et d'effectuer une calibration de la mise au point. À la fin de cette procédure le microscope sera prêt pour l'acquisition.

UI Expand

title	Première mise au point

On the microscope touch screen :

Press sur Full Operation

Press 4x to select the 4x objective

Info
The 4x objective is the safest because it has the greatest working distance (16 mm).The sample will appear perfectly sharp long before the lens approaches it.It is recommended to always first focus with the safest lens.The objectives are para-focal, focusing with the safest objective will then allow you to easily find your sample with another objective.

Press Escape to raise the lenses to their highest position
Place the test slide on the microscope stage with the coverslip toward the objective
Remarque
title Important
Always use the test slide to perform the first focus.
If necessary, move the stage so that the sample is centered on the objective

On the computer

Open the CellSens Dimension software
Wait for the software to open (yes it's long...)
At the bottom right of the window, select BF or the desired fluorescence (DAPI, FITC, TRITC or mCherry) to activate the configuration
Adjust the focus with the main dial while looking through the eyepieces until the image is perfectly sharp
Remarque
The focus is around Z = 19000 um. The Z position value is visible on the touch screen.
At the bottom right of the window, select Off to turn off the illumination

UI Expand

title	Secondary focus

Avertissement

title	Important

First focus with the safest lens before selecting another lens and continuing with secondary focus.

UI Expand

title	Focusing with air lenses

After performing the first focus, on the microscope touch screen:

Press 10x, 20x or 40x to select the desired lens

Info
The 40x objective is the best Air objective because it has the greatest number of optical corrections (Plan Apochromat) and the largest numerical aperture (0.95).It offers a lateral resolution of 420nm at a wavelength of 550nm.

At the bottom right of the window, select BF or the desired fluorescence (DAPI, FITC, TRITC or mCherry) to activate the configuration
Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
Select Off to turn off the illumination
Your sample is ready for acquisition!

UI Expand

title	Focusing with oil lenses

After performing the first focus, on the microscope touch screen:

Press 60x Oil (1.35), 100x Oil (1.4) to select the desired lens.The microscope will automatically raise the objectives so that the sample is accessible.

Info
The 100x objective is the best oil objective because it has the highest number of optical corrections (Plan Apochromat) and the largest numerical aperture (1.4).It offers a lateral resolution of 240nm at a wavelength of 550nm.

Place a drop of oil on your sample

In the CellSens Dimension software

Click OK
At the bottom right of the window, select BF or the desired fluorescence (DAPI, FITC, TRITC or mCherry) to activate the configuration
Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp
Select Off to turn off the illumination
Your sample is ready for acquisition!

UI Expand

title	Storage management

Files can be saved temporarily (during acquisition) to local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create one folder per laboratory using the principal investigator's last name. Inside, create a folder per user using the following nomenclature (First Name_Last Name).

Remarque

title	Important

In any case, do not store your files on the C: drive.

UI Expand

title	Shutdown

Save your data
Close the software CellSens Dimension
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
Turn off the computer
If oil objectives were used, clean it with lens cleaner and lens paper (not Kimwipes)
Turn off the microscope power bar (#2)
Replace the dust cover on the microscope

Remarque

title	Important Reminders

Collect your samples, especially those in the microscope
Leave the microscope and workspace clean

Tabs Page

id	Light path
title	Light path

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.

Tabs Page

id	Manuals
title	Manuals

Available manuals

Tabs Page

id	Log
title	Log

UI Expand

expanded	true
title	To do

Create BF light path
Buy multi-bandpass filters

UI Expand

title	2024-02-12

Complete installation setup
Scrip for CellSens personalization

UI Expand

title	2024-01-18

System relocated to Room 5308-2
Added to Faces
XCite NOVEM-S Installed

Tabs Page

id	Technical Datasheet
title	Technical Datasheet

Stand

Olympus BX63 Serial 0G00080

Condenser

Motorized condenser

Camera

Olympus DP80 Serial 8K48090

Stage

Motorized stage SHe Serial 127808

Workstation

HP Z4 Workstation
Intel Xeon W-2102 @ 2.9GHz
RAM 32 GB DDR4 2400 MHz
OS 1 TB GB SSD 550 MB/s
2 TB HD Data Storage (2 x 1 TB spanned volume) 162 MB/s
Video Card nVidia Quadro P620 2GB DDR5 dedicated memory
Monitor Dell P2415Q display 24' 3840 x 2160
Software CellSens Dimension v3.2

Consumables

This can happen when the liquid running through the chamber is not flowing properly.

Pause your experiment
Turn off the Okolab environment controller 3A and 3B
Disconnect the blue end of the connection pipe (at the junction with the spring shape objective warmer)
Place the open end into a dish to collect liquid
Turn the Lauda water bath back ON (3B)
The liquid should flow quite rapidly, if not proceed as follow
- Turn OFF the Lauda water bath (3B)
- Take the 20 mL syringe located inside the drawer labelled Tubing
- Connect it to the open end of the blue pipe
- Use the syringe to blow pressurized air into the pipes to clear it out
- Remove the syringe (and store it back into the Tubing drawer)
- Test if the liquid is now flowing properly by turning the Lauda water bath back ON (3B)
  - If not repeat the procedure
  - If so, turn OFF the Lauda water-bath (3B)
  - Reconnect the blue and green pipes together
  - Turn the Okolab environment controller 3A and 3B back ON

Tabs Page

id	FAQ
title	Troubleshooting & FAQ

Troubleshooting

UI Expand

title	The microscope does not turn on, what should I do?

Please ensure that the microscope touchscreen is turned on. The switch is not easily accessible and is located on the back at the right of the touchscreen pad.

FAQ

UI Expand

title	Can I use this microscope to look at cell in a dish?

No. This is an upright microscope designed to look at specimen mounted between a slide and a coverslip

Tabs Page

id	FAQ
title	Troubleshooting & FAQ

Troubleshooting

UI Expand

title	Chamber does not reach the desired temperature (or is very slow to warm up)

Avertissement
Be careful not to touch your sample when performing these actions as it may displace your current acquisition

Info

title	How the Okolab chamber works

The liquid from the water bath enters the lead first via the red pipe
Then it goes through the lead circuitry to keep it warm
It exists via the unlabeled tube and enters the incubation chamber main body
It goes through the circuitry of the main body and exits via the green labelled tube

One must maintain a decent amount of liquid in the Lauda water bath to avoid bubble formation in the circuitry

Because the circuitry inside the top lead and the incubation chamber main body is thin it can get clogged. The procedure above can solve this issue.

UI Expand

title	Liquid forming inside the incubation chamber

This happens when the mixed-gas humidifier is overfilled. Bubbles created by the gas going through the humidifier can bring liquid into the gas feed line.

Turn off the Okolab module and the water bath
Remove your sample and store it properly
Dry the incubation chamber with a clean tissue
Carefully remove the cap of the humidifier glass bottle

Avertissement
Pay extra care when manipulating the humidifier bottle as it is made of glass and is very fragile

Remove distilled water from the humidifier

Info
Humidifier shouldn't be more than 2/3^rd filled

Close the humidifier by replacing the cap
Turn on the Okolab module and the water bath

UI Expand

title	Liquid forming inside the incubation chamber but the humidifier is not overfilled

This can happen during long experiments. The high humidity of the gas mixture condensates in the pipe between the humidifier and the incubation chamber.

Pause your experiment
Disconnect both ends of the yellow tube from the humidifier
Drain the tube from any liquid
Reconnect the yellow tube to the humidifier
You can use a syringe or gas pressure to blow out any liquid from the incubation chamber cover

Avertissement
Be careful not to touch your sample when performing this action

Wipe any liquid with a tissue
Reconnect the yellow tube to the incubation chamber

FAQ

UI Expand

title	Can I use this microscope to look at cell in a dish?

Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
The objectives are optimized to image through thin glass bottom multi-well plates
You may also image specimen mounted between a slide and a 0.17mm thick coverslip
For long timelapse, be aware of photo-toxicity

UI Expand

title	How can I warm up the incubation chamber faster?

You may use a trick to warmup the incubation chamber faster. Using this method. you should reach a stable temperature within 30 minutes.

Avertissement
Do NOT proceed with your sample but with a blank control (dish with distilled water for example)

Place your blank control in the incubation chamber
Immerge the tip of the sample temperature probe in the blank
Set the Okolab temperature Control Mode to Chamber (Settings>Temperature>Control Mode>Chamber>Save)
Set the Okolab temperature to 50°C (Home>Temperature>50°C>Set)
Check the temperature of the sample (Menu Magnifier>Sample Temperature). It should take between 10 to 15 minutes for the blank to reach 30°C.

Info
The Lauda water bath is faster to warm up water than to cool it down. Make sure to anticipate and not pass beyond the desired temperature. If your desired temperature is 37°C, you can stop the procedure when the blank has reached 30°C.

When the blank has reach the desired temperature

Set the Okolab temperature back to 37°C (Home>Temperature>37°C>Set)

Set the Okolab temperature Control Mode to Sample (Settings>Temperature>Control Mode>Sample>Save)

Wait 10 to 20 minutes until the temperature stabilizes

Then you can safely replace the blank with your sample

Tabs Container

id	Demo Image
title	Olympus BX63
direction	horizontal

Inclure page

	Plateformes:_foot-en
	Plateformes:_foot-en

Raccourcis espace

Arborescence des pages

Comparaison des versions

Ancienne version 7

Nouvelle version Actuel

Légende

Olympus BX63 upright microscope

Stand

Light sources

Condenser

Objectives

Stage

Detector

Workstation

Incubation

Consumables

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.

Stand

Condenser

Camera

Stage

Workstation

Consumables

Troubleshooting

FAQ

Raccourcis espace

Arborescence des pages

Historique de la page

Comparaison des versions

Ancienne version 7

Nouvelle version Actuel

Légende

Olympus BX63 upright microscope

Stand

Light sources

Condenser

Objectives

Stage

Detector

Workstation

Incubation

Consumables

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.

Stand

Condenser

Camera

Stage

Workstation

Consumables

Troubleshooting

FAQ