If fluorescence was used, turn off the X-Cite lamp (#4A)

When using for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example.

1. If open, close the Zen Blue software and wait for it to close completely (up to 30 seconds)
2. On the Desktop open the Documentation folder
3. Double-click Settings for Axio-Osberver Z1
4. A script will run and a black window will appear briefly
5. You can then reopen the Zen Blue software

• Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
• At the end of each session, copy your data to your external drive and delete it from the local C: drive
• You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

1. Save your data
2. Close Zen Blue
3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive

4. Turn off the microscope power bar (#2)

5. Turn off the computer
6. Cover the microscope

The first time you use the instrument, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.

Available manuals

• Zen Quick Guide (pdf)
• AxioCam MR Reference Guide (pdf)
• Zen Blue v2.0 software guide (pdf)

Stand

• Zeiss Axio-Observer Z1 inverted  Serial: 3851001242 Part Number: 431007-9902-000
  System ID: 1024979772

• Camera adapter Model 60N-C, 1", 1x, Model: 426114

Light sources

• Transmitted LED light
  • TBD Filters
• X-Cite 120Q Serial: TBD

Condenser

• Manual condenser Product number: TBD, Serial: TBD

• Lens NA 0.35 WD 70 mm Part Number: 424241

• Filter turret 6 positions manual

  1. H

  2. Ph0

  3. Ph1

  4. Ph2

  5. DIC

  6. DUC

Objectives

• 2.5x/0.085 Air WD 8.8

• Empty

• 10x/0.25 Air Ph1 WD 6.5

• Empty
• 20x/0.5 Air Ph2 WD 2.0
• 40x/0.95 Air WD 0.25

Stage

• Motorized stage Marzhauser Sensotech, Part number 432903-9011-000, #14 07 132052; 90-76-200-0820
• Remote control joystick
• Inserts
  
  • Slide combo
  • 6-well plate
  • 35 mm dish
  • Multi-well plat

Filters

1. DAPI Filter Set 49
2. GFP Filter Set 13
3. Rhodamine Filter Set 43
4. DHE (dihydroethidium)
5. Cy5 Filter Set 50
6. Empty

Detector

• Zeiss AxioCam MR R3 CCD Camera 1388 x 1040 pixels, 12-bit, 13 images/s at full resolution, detector size 8.9 mm x 6.7 mm. Model: r3.1 Part Number: 426509-9901-000. Serial: 1 22 12 5537

Workstation

• Fujitsu Esprimo P920 E90+
• Intel Core i5-4670 @ 3.4 GHz
• RAM 32 GB DDR3 1600 MHz ECC (4 x 8 GB)
• OS 500 GB SSD 550 MB/s
• 2 TB HD Data Storage (2 x 1 TB spanned volume) 110 MB/s
• Video Card AMD FirePro V4900 1 GB DDR5 dedicated memory
• Monitor TBD display TBD' 1920 x 1200
• Software Zen Blue 2.0

Incubation

• Pecon incubation
• TBD

Consumables

• CO₂ Tank
• N₂ Tank
• Liquid Light Guide
• 100 W Mercury lamp

Troubleshooting

FAQ