HP Z840 Poste de déconvolution

Roger Gaudry R-619

Applications
- Déconvolution 2D et 3D
- Traitement d'images
- Analyse d'images
- Quantification
Processeurs
- 2 x Intel Xeon E5-2623 v4 2.60 GHz
RAM
- 128 GB (8x16GB) ECC DDR4 2400 MHz
Disques
- 512 GB NVMe à 2500 MB/s OS
- 4 TB (2x2TB) à 170 MB/s volumes étendus
Carte vidéo
- NVidia GTX 1080 avec 8GB de mémoire DDR5 dédiée
Écran
- HP Z24n 24" IPS avec une résolution native de 1920 x 1200
Système d'exploitations
- Windows 10 21H1
Logiciels
- ImageJ
- FIJI
- Microsoft Office 2016
- NIS-Elements AR v5.02 64-bits
- NIS-Elements Offline Deconvolution v4.11 64-bits

Enregistrez vos données
Fermez le logiciel NIS-Elements Offline Deconvolution
Transférez vos données sur le disque D: (Data Storage) ou sur votre disque dur externe et les supprimer du disque local C:

Les fichiers peuvent être sauvés temporairement (pendant l'utilisation) sur le disque local C: (bureau)
À la fin de chaque session, copiez vos données sur votre disque externe et supprimez-les du disque local C:
Vous pouvez stocker vos fichiers sur le disque D: (Data Storage). Si vous utilisez ce disque, veuillez créer un dossier par laboratoire en utilisant le nom de famille du chercheur principal. A l’intérieur créez un dossier par utilisateur (Prénom_Nom).

Dans tous les cas, ne stockez pas vos fichiers sur le disque C:

Manuels disponibles version anglaise.

Windows 10 UdeM Image installation
Change the 2nd network adapter configuration with fix IP to enable for computer to computer communication
Change sharing policies of public document folder

Transmitted light Halogen 12V 100W Serial 01875599
Reflected light
- Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
- Bulb housing model LH M100C-1 Serial 039326

Position	Name	Brand	ID	Magnification	Numerical Aperture	Immersion	Type	Working distance (mm)	Transmittance (% [nm])	Technique	Cover glass thickness (mm)
1	10x/0.3 Air	Nikon	10x/0.3 Air Plan Fluor Ph1 DLL	10x	0.3	Air	Plan Fluor	16	>75% [400-800]	BF, Pol, PhC, Fluo	0.17
2	40x/0.75 Air	Nikon	40x/0.75 Air Plan Fluor Ph2 DLL	40x	0.75	Air	Plan Fluor	0.72	>80% [400-750]	BF, Pol, PhC, Fluo	0.17
3	100x/1.3 Oil	Nikon	100x/1.3 Oil Plan Fluor Ph3 DLL	100x	1.3	Oil	Plan Fluor	0.2	>75% [400-800]	BF, Pol, PhC, Fluo	0.17
4	Empty
5	Empty
6	Empty

Position	Cube name	Brand	ID	Excitation Filter	Dichroic mirror	Emission Filter
1	DAPI/Hoechst/AMCA	Chroma	Cube set 31000v2	350/50x [325-375]	400LP	460/50m [435-485]
2	FITC/EGFP	Chroma	Cube set 41001	480/40x [420-500]	505LP	535/50m [510-560]
3	TRITC/Rhodamine	Chroma	Cube set 41002c	545/30x [530-560]	570LP	620/60m [590-650]
4	Texas Red	Chroma	Cube set 41004	560/55x [532-588]	595LP	645/75m [607-682]
5	Empty
6	Empty

Deconvolution processing not giving expected results...

This usually happens when the image files do not contain the correct metadata.

Make sure to save the files with all available metadata on the microscope used for the acquisition.
This usually requires to save the files in the software propriatery format (.nd for NIS-Elements and Metamorph, .czi for Zen,...)
You can always add the correct calibration values after acquisition (pixel size in XY and Z, wavelength, Z-step)

Can I use this workstation remotely?

Yes and No. You can't use the software remotely but you can start the software locally and connect remotely to make sure all is running smoothly. To do so you need to request permission to the platform manager.

Which deconvolution method should I use?

There is not a single answer
If you have a single plan you should choose 2D deconvolution. If you have a Z-stack you should pick 3D deconvolution.
If you want to have a quick view of the result you should use Fast Deconvolution.
For best results Landweber deconvolution
For more Yes and No. You can't use the software remotely but you can start the software locally and connect remotely to make sure all is running smoothly. To do so you need to request permission to the platform manager.

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