Nikon Eclipse E-600 upright microscope
Roger Gaudry N-620
- Applications
- Brightfield
- Phase Contrast
- Polarized light
- Fluorescence
Light sources
Halogen lamp for transmitted light
Mercury lamp (350~600nm) for fluorescence
Objectives:
10x/0.3 Air Ph1
40x/0.75 Air Ph2
100x/1.3 Oil Ph3 WD 0.2
Filter cubes
- DAPI/Hoechst/AMCA
- FITC/EGFP
TRITC/Rhodamine
TxRed
- Detector
Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6fps at full frame
Startup
Turn on the computer
Turn on the microscope power bar
If fluorescence is required, turn on the mercury lamp power supply unit (3A) and press ignition (3B)
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
Log in Windows using your UdM credentials
Start-up NIS-Elements
The first time you log in the computer, you need to import the microscope settings. To do this follow the instructions Nikon Eclipse E600-en
Shutdown
Save your data
Close NIS-Elements
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
Get your samples from the microscope
If used, clean the oil objective with lens cleaner and lens paper
If fluorescence was used, turn off the mercury lamp power supply unit (3A)
Turn off the microscope power bar (2)
Wait until the lamps are cool and cover the microscope
Important Reminders
Take back your samples including ones in the microscope
Leave the microscope and the working area clean
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
Storage Management
Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
In any case, your files should be removed from the C: drive.
Setting-up NIS-Elements
This is required the first time you are using the instrument. You will usually do it during the training session. It can be performed if something is not working properly right or if you want to refresh the software interface.
This process will delete all experiment protocols and restore the parameters for the microscope
Close NIS-Elements
Wait until the complete closure of NIS-Elements
Open the folder Desktop\Logiciels
Open the software NIS Settings Utility
Click on the Import tab
Click on Browse
Navigate to your desktop
Select the file Nikon-E600 Settings.bin
Click Select
Select all items
Click Import
Click OK
Close the NIS Settings
Follow the schematics below for Transmitted light (Bright field, Phase Contrast, Polarized light) and transmitted light (fluorescence).
Nikon_Eclipse-E600_Polarization_Manual.pdf
Nikon_Eclipse-E600_Epi Fluo D_Manual.pdf
Nikon_Eclipse-E600_Epi Fluo Y_Manual.pdf
To do
Adjust focus drive jitter
Add a holder for polarized light analyzer
2021-07-20
Replacement mercury lamp bulb HBO 1003W/2
256 GB SSD added in workstation for OS
Windows 10 Installation
BIOS updated to v2.47
Camera Firmware updated to v2.11
NIS-Elements Basic Research v4.6 64-bits installed
Creation NIS Elements Settings
FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK
2018-04-04
Nikon Preventive Maintenance
Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
100x slightly damaged but not the lens
Fluorescence cubes damaged: FITC TxRed
Focus drive has a jutter
Stand
Nikon Eclipse E600 upright Serial 725540
Light sources
Transmitted light Halogen 12V 100W Serial 01875599
Reflected light
Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
Bulb housing model LH M100C-1 Serial 039326
Condenser
Condenser Universal C-CU Serial 081901
Lens Dry NA 0.9
Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty
Objectives
1
40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air (Transmission curve) with PF40 Wollaston prism
100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil (Transmission curve) with PF/PA 100 Oil Wollaston prism
Empty
Empty
Empty
Turret
| Position | Cube name | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter |
|---|---|---|---|---|---|---|
| 1 | DAPI/Hoechst/AMCA | Chroma | Cube set 31000v2 | 350/50x | 400LP | 460/50m |
| 2 | FITC/EGFP | Chroma | Cube set 41001 | 535/50m | ||
| 3 | TRITC/Rhodamine | Chroma | 545/30x | 570LP | 620/60m | |
| 4 | Texas Red | Chroma | 595LP | 645/75m | ||
| 5 | Empty | |||||
| 6 | Empty |
Detector
Camera Nikon DS-Ri2 Serial 701234
Workstation
HP Z440 Workstation
Intel Xeon E5-1630 v3 @ 3.7GHz
RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
OS 256GB SSD 550 MBs
2TB HD Data Storage 150 MBs
Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
Monitor HP Z24i display 24' 1920x1200
Consumables
Mercury lamp bulb OSRAM HBO 1003W/2 100W
Tungsten Halogen Lamp OSRAM 64623 HLX 100W 12V
Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)
Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)
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