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iconbuild
titleFrançais
typeprimary
urlhttps://wiki.umontreal.ca/display/Microscopie/Nikon+Ti2-E

Tabs Container
idInstrument Tabs
titleNikon Ti2-E
directionhorizontal
Tabs Page
idDescription
titleDescription

Nikon TE2000-E inverted microscope

Bombardier Building, Room 3132
Advanced Microscope Tier 1 usage price

Instrument awarded to Dr. Steve Michnick by

the

the Canadian Foundation for Innovation (CFI)

Advanced Microscope Tier 1 usage price

Applications

  • Transmitted light
, Bright-field,
  • Phase Contrast

  • Fluorescence

    • Interference Contrast (DIC)
    • Fluorescence
    • Widefield imaging
    • Live imaging
    • Time-lapse imaging

  • Light sources

    • Halogen lamp for transmitted light

    • X-Cite exacte for fluorescence

    • Objectives

      1. 4x/0.1 Air
      2. 10x/0.30 Air Ph1
      3. 20x/0.45 Air Ph1
      4. 40x/0.95 Air
      5. 60x/1.4 Oil Ph3
      6. 100x/1.45 Oil
    Expand
    titleObjectives complete specifications
    PositionNameBrandFull nameIdentifierWorking distance (mm)Transmittance
    (% [nm])TechniquesCover glass thickness (mm)14x/0.10 AirNikon

    Image AddedImage Added


    Tabs Container
    idInstrument Tabs
    titleNikon Ti2-E
    directionhorizontal


    Tabs Page
    idDescription
    titleDescription

    Nikon TE2000-E inverted microscope

    Bombardier Building, Room 3132
    Instrument awarded to Dr Steve Michnick by the Canadian Foundation for Innovation (CFI)
    Advanced Microscope Tier 1 usage price

    • Applications

      • Transmitted light, Bright-field, Phase Contrast, DIC

      • Fluorescence

      • Live imaging
      • Time-lapse imaging

    • Light sources

      • Halogen lamp for transmitted light

      • X-Cite exacte for fluorescence

    • Objectives

      1. 4x/0.1
    Plan

    MRL00042

    30>80% [400-900]BF, Fluo-210x
      1. Air
      2. 10x/0.30 Air Ph1
    Nikon
      1. 20x/0.
    30 Plan Fluor
      1. 45 Air Ph1
    DL

    MRH20101
    MRH20105

    15.2>80% [440-700]BF, PhC, Fluo1.2 !
      2. 40x/0.95 Air
      3. 60x/1.4 Oil Ph3
      4. 100x/1.45 Oil
    Expand
    titleObjectives complete specifications


    PositionNameBrandFull nameIdentifierWorking distance (mm)Transmittance
    (% [nm])    		TechniquesCover glass thickness (mm)
    14x/0.10 AirNikon4x/0.1 Plan

    MRL00042

    		30>80% [400-900
    320x/0.45 Air Ph1Nikon20x/0.45 Plan Fluor ELWD Ph1 ADL

    MRH48230
    MRH48220

    7.4>80% [350-870]BF, PhC, Fluo0-2440x/0.95 AirNikon40x/0.95 Plan Apo DIC M/N2

    MRD00400
    MRD70470

    0.14>80% [440-760
    ]BF, Fluo
    0.11
    -
    0.23
    5
    2
    60x
    10x/
    1.4 Oil Ph3
    0.30 Air Ph1Nikon
    60x
    10x/
    1
    0.
    4
    30 Plan
    Apo
    Ph3 DM

    MRD31600

    0.21
    Fluor Ph1 DL

    MRH20101
    MRH20105

    		15.2>80% [440-700]
     
    BF, PhC, Fluo
    0
    1.
    17
    2 !
    6
    3
    100x
    20x/
    1
    0.45
    Oil
    Air Ph1Nikon
    100x
    20x/
    1
    0.45 Plan
    Apo Lambda  OFN25 DIC N2

    MRD01905
    MRD71970

    Fluor ELWD Ph1 ADL

    MRH48230
    MRH48220

    		7.4
    0.13
    >80% [
    460
    350-
    720
    870]BF, PhC, Fluo0
    .17

     Also available

    PositionNameBrandFull nameIdentifierWorking distance (mm)Transmittance
    (% [nm])TechniquesCover glass thickness (mm)-40x/0.60 Air Ph2NikonPlan Fluor 40x/0.60 Ph2 DIC M ELWD 0-2

    MRH48430

    2.7-3.7
    -2
    440x/0.95 AirNikon40x/0.95 Plan Apo DIC M/N2

    MRD00400
    MRD70470

    		0.14>80% [440-760]BF, Fluo0.11-0.23
    560x/1.4 Oil Ph3Nikon60x/1.4 Plan Apo
    Ph3 DM

    MRD31600

    		0.21 
    >80% [350-850]
    BF, PhC, Fluo0
    -2
    .17
    -
    6
    40x
    100x/
    0
    1.
    60 Air
    45 OilNikon
    S Plan Fluor 40x/0.6 ELWD DIC N1

    MRH08430

    2.8-3.6
    100x/1.45 Plan Apo Lambda  OFN25 DIC N2

    MRD01905
    MRD71970

    		0.13>80% [460-720
    >80% [350-850
    ]BF, Fluo0
    -2-40x/0.90 AirNikonS Fluor 40x/0.90 DIC M/N2

    NA

    0.3NA
    BF, Fluo0.11-0.23-
    .17

     Also available

    PositionNameBrandFull nameIdentifierWorking distance (mm)Transmittance
    (% [nm])    		TechniquesCover glass thickness (mm)
    -40x/0.60 Air Ph2
    60x/0.70 Air
    NikonPlan Fluor
    60x
    40x/0.
    70 ELWD
    60 Ph2 DIC M
    /N1

    MRH08630

    ELWD 0-2

    MRH48430

    		2.7-3.7
    1.5-2.1
    >80% [
    380
    350-
    880
    850]BF, PhC, Fluo0
    .5
    -
    1.5
    2
    -
    60xA
    40x/
    1
    0.
    40 Oil
    60 AirNikonS Plan
    Apo 60xA
    Fluor 40x/
    1
    0.
    40 Oil
    6 ELWD DIC
    H
    N1

    MRD71670

    MRH08430

    		2.8-3.6
    0.21
    >80% [
    480
    350-
    700
    850]BF, Fluo0
    .17
    -2
    -
    60x
    40x/
    1
    0.
    4 OilMRD71670
    90 AirNikon
    Plan Apo VC 60x/1.40 Oil DIC N2
    S Fluor 40x/0.90 DIC M/N2

    NA


    		0.
    13>80% [480-700]
    3NA
    		BF, Fluo0.
    17
    11-0.23
    -
    100x
    60x/0.
    85
    70 AirNikon
    L
    Plan
    100x
    Fluor 60x/0.
    85 ODN 25

    MUE35900

    0.85 0-0.7-
    70 ELWD DIC M/N1

    MRH08630

    		1.5-2.1>80% [380-880]BF, Fluo0.5-1.5
    -60xA/1.40
    100x/1.4
    OilNikonPlan Apo
    100x
    60xA/1.
    4
    40 Oil DIC H
    MRD71970

    MRD71670

    		0.
    13
    21>80% [480-
    720
    700]BF, Fluo0.17

    • Filters

      1. Dichroic DAPI\FITC\TRITC

      2. Dichroic CFP\YFP\Cy5

      3. Dichroic CFP\YFP\mCherry

      4. DAPI
      5. Cy3n
      6. Cy5.5
    • Also available

      • CFP

      • FITC

      • YFP

      • Cy3.5

      • mCherry

      • Cy5

    Expand
    titleFilters complete specifications

    Filter cubes

    PositionNameBrandIDExcitation FilterDichroic mirrorEmission FilterComments1Dichroic
    DAPI\FITC\TRITCChroma86013 v286013bsTo be used in combination with excitation and emission filters
    -60x/1.4 OilNikonPlan Apo VC 60x/1.40 Oil DIC N2

    MRD71670

    		0.13>80% [480-700]BF, Fluo0.17
    -100x/0.85 AirNikonL Plan 100x/0.85 ODN 25

    MUE35900

    		0.85 
    		0-0.7
    -100x/1.4 OilNikonPlan Apo 100x/1.4 Oil DIC H

    MRD71970

    		0.13>80% [480-720]BF, Fluo0.17


    • Filters

      1. Dichroic DAPI\FITC\TRITC

      2. Dichroic CFP\YFP\Cy5

      3. Dichroic

    2

    Dichroic
    CFP\YFP\Cy5

    Chroma86008 v2

    86008bs

    To be used in combination with excitation and emission filters3Dichroic

      1. CFP\YFP\mCherry

    Chroma4DAPIChroma

    31000 v2

    AT350/50x

    400dclp

    D460/50m

    5Cy3nChroma

    SP102 v1

    HQ546/11xQ557lp

    HQ567/15m

    6Cy5.5Chroma

    41022

    HQ665/45x

    Q695lpHQ725/50m

     Excitation filters

    PositionNameBrandIDExcitation FilterMatching dichroic mirrorMatching
      1. DAPI

    89006 ET

    69008bsTo be used in combination with excitation and emission filters
      1. Cy3n
      2. Cy5.5
    • Also available

      • CFP

      • FITC

      • YFP

      • Cy3.5

      • mCherry

      • Cy5

    Expand
    titleFilters complete specifications

    Filter cubes

    PositionNameBrandIDExcitation FilterDichroic mirror
    Emission FilterComments
    1Dichroic
    DAPI\FITC\TRITC    		Chroma86013 v2
    Exc 1
    AT350/50x

    86013bs

     S457/17m

    		86013bs
    		To be used in combination with excitation and emission filters
    2

    Dichroic
    CFP\YFP\Cy5

    2CFP

    		Chroma86008 v2
    Exc 1S430/25x


    86008bs

    69008bsS465/30m3FITCChroma86013 v2 Exc 2

    S484/15x

    86013bs

    S517/30m


    		To be used in combination with excitation and emission filters
    3

    Dichroic
    CFP\YFP\mCherry

    		Chroma

    89006 ET


    		69008bs
    		To be used in combination with excitation and emission filters
    4DAPIChroma

    31000 v2

    AT350/50x

    400dclp

    D460

    4

    YFP

    Chroma86008 v2 Exc 2

    S510/20x

    86008bs
    69008bs

    S550

    /50m


    5
    TRITC
    Cy3nChroma
    86013 v2 Exc 3S555/25x

    86013bs

    SP102 v1

    		HQ546/11xQ557lp

    HQ567/15m

    S605/40m


    6
    mCherry
    Cy5.5Chroma
    89006 ET Exc 3

    ET572/35x

    69008bs

    ET632/60m

    41022

    HQ665/45x

    		Q695lpHQ725/50m


     Excitation

    7

    Cy5

    Chroma86008 v2 Exc 3

    S622/36x

    86008bs

    S700/75m8

    Empty

    Emission

    filters

    PositionNameBrandID
    Matching
    Excitation FilterMatching dichroic mirrorMatching Emission FilterComments
    1DAPIChroma86013 v2
    Em
    Exc 1AT350/50x

    86013bs

         		S457/17m
    2

    CFP

    		Chroma86008 v2
    Em
    Exc 1

    S430/25x

    86008bs
    69008bs

    		S465/30m
    3FITCChroma86013 v2
    Em
    Exc 2

    S484/15x

    86013bs

    S517/30m


    4

    YFP

    Semrock
    Chroma86008 v2 Exc 2
    FF01-550/49-26

    S510/20x

    86008bs
    69008bs

    F01-550/49-25

    S550/50m


    5TRITCChroma86013 v2
    Em
    Exc 3S555/25x

    86013bs

    S605/40m


    6

    mCherry

    Semrock
    Chroma89006 ET Exc 3
    FF01-641/75-25

    ET572/35x

    69008bs

    FF01-641/75-25

    ET632/60m


    7

    Cy5

    		Chroma86008 v2
    Em
    Exc 3

    S622/36x

    86008bs

    		S700/75m
    8

    Empty

    9

    Shutter

    10

    Shutter

     Also available








    Emission filters

    PositionNameBrandIDMatching Excitation Filter
    Dichroic
    Matching dichroic mirrorEmission FilterComments
    -
    1
    eCFP
    DAPIChroma

    49001 ET

    ET436/20x

    T455lp

    ET480/40m

    86013 v2 Em 1AT350/50x

    86013bs

         		S457/17m
    2

    CFP

    		Chroma86008 v2 Em 1

    S430/25x

    86008bs
    69008bs

    		S465/30m
    3
    -cGFPChroma

    31044 v2

    D436/20x

    455dclp

    D480/40m

    -
    FITCChroma

    41001

    HQ480/40x

    Q505lp

    HQ535/50m

    86013 v2 Em 2

    S484/15x

    86013bs

    S517/30m


    4
    -

    YFP

    Chroma

    49003 ET

    SemrockFF01-550/49-26

    S510

    ET500

    /20x

    T515lp

    86008bs

    ET535/30m

    69008bs

    F01-550/49-25

    Cy3.

    5TRITCChroma

    SP103 v1

    HQ581/10x

    Q593lp

    86013 v2 Em 3S555/25x

    86013bs

    S605

    HQ617

    /40m

    -

    6

    mCherry

    Chroma

    49008 ET

    ET560/40x

    T585lpxr

    ET630/75m

    SemrockFF01-641/75-25

    ET572/35x

    69008bs

    FF01-641/75-25


    7
    -

    Cy5

    		Chroma

    49006 ET

    ET620/60x

    T660lpxr

    86008 v2 Em 3

    S622/36x

    86008bs

    		S700
    ET700
    /75m

    • Detector

      • Hamamatsu ORCA Flash v2 CMOS Monochrome Camera 2048x2048 pixels, 16-bit, 30 images/s at full frame (100 images/s at full frame with camera link connector) Serial 002162

    Tabs Page
    idUser Guide
    titleUser Guide
    UI Expand
    expandedtrue
    titleStartup
    1. Turn on the computer (#1)
    2. Remove the cover from the microscope
    3. Turn on the X-Cite exacte light (#2)
    4. Turn on the microscope power bar (#3)
    5. Use your UdeM credentials to log in to Windows
    6. The first time you use the instrument, you need to import the microscope settings into the software. To do this follow the instructions First use protocol.
    7. Start the software NIS
    UI Expand
    titleShutdown
    1. Save your data
    2. If the oil objective was used, clean it with lens cleaner and lens paper (not Kimwipes)
    3. Select the lowest magnification objective
    4. Close the software NIS
    5. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
    6. Turn off the computer
    7. Turn off the X-Cite exacte light (#2)
    8. Turn off the microscope power bar (#3)
    9. Cover the microscope

    8

    Empty







    9

    Shutter







    10

    Shutter







     Also available

    PositionNameBrandIDExcitation FilterDichroic mirrorEmission FilterComments
    -eCFPChroma

    49001 ET

    ET436/20x

    T455lp

    ET480/40m


    -cGFPChroma

    31044 v2

    D436/20x

    455dclp

    D480/40m


    -FITCChroma

    41001

    HQ480/40x

    Q505lp

    HQ535/50m


    -YFPChroma

    49003 ET

    ET500/20x

    T515lp

    ET535/30m


    -Cy3.5Chroma

    SP103 v1

    HQ581/10x

    Q593lp

    HQ617/40m


    -mCherryChroma

    49008 ET

    ET560/40x

    T585lpxr

    ET630/75m


    -Cy5Chroma

    49006 ET

    ET620/60x

    T660lpxr

    ET700/75m


    • Detector

      • Hamamatsu ORCA Flash v2 CMOS Monochrome Camera 2048x2048 pixels, 16-bit, 30 images/s at full frame (100 images/s at full frame with camera link connector). Spectral response >40% between 400-850nm. Max 80% at 550nm.

        Image Added


    Tabs Page
    idUser Guide
    titleUser Guide
    UI Expand
    expandedtrue
    titleStartup
    1. If not already done, turn on the computer (#1) and use your UdeM credentials to log in to Windows
    2. Remove the cover from the microscope
    3. Turn on the X-Cite exacte light source (#2)
    4. Turn on the microscope power bar (#3)
    5. When using the software for the first time, it is necessary to import the microscope-specific settings before launching the acquisition software. Please refer to the First Use section below for instructions.
    6. Start NIS-Elements
    UI Expand
    titleFirst use

    When using the acquisition software for the first time, it is necessary to import the microscope-specific parameters into the software. This procedure is usually carried out during the training session.However, it is also possible to use it to reset the software if it is not displayed correctly, for example.

    Note

    Please note, this procedure will delete all your experiment protocols and restore the software to its original settings.

    1. If open, close NIS-Elements and wait until it is completely closed
    2. On the Desktop open the folder Softwares
    3. Open NIS Settings Utility
    4. Click on the Import tab
    5. Click on Browse
    6. Navigate to your Documents
    7. Select the file NIS Settings for Nikon TE2000E.bin
    8. Click Select
    9. Select all items
    10. Click Import
    11. Click OK
    12. Close NIS Settings Utility
    13. You can then open NIS-Elements
    UI Expand
    titleLoading samples

    This procedure puts the microscope in a safe configuration to load your sample. At the end the microscope will be ready for acquisition.

    UI Expand
    titleFirst focus
    1. If not already done, in NIS-Elements select 4x to select the 4x objective
      Info

      The 4x objective is the safest because it has the longest working distance (30mm). The sample will appear perfectly sharp long before the lens approaches it. It is recommended to always first focus with the safest lens. The objectives are parafocal, focusing with the safest objective will then allow you to easily find your sample with another objective. The 10x objective is also safe because its working distance is 15.2 mm.

    2. If not done already, press Escape to lower the objective to the lowest position
    3. Place the test slide on the microscope stage with the coverslip toward the objective
      Note
      titleImportant

      Always use the test slide to perform the first focus.

    4. If necessary, move the stage so that the sample is centered on the objective
    5. In NIS-Elements, select the optical configuration BF vis or the desired fluorescence (DAPI vis, ...) to activate the configuration
    6. Adjust the focus with the main dial while looking through the eyepieces until the image is perfectly sharp
      Note

      The focus can be found at Z = mm). The Z value can be found in the NIS Software XYZ Position tab.


    7. In NIS-Elements, select the optical configuration Off to turn off the illumination


    UI Expand
    titleSeconday focus
    Warning
    titleImportant

    First focus with the safest objective before selecting another lens and continuing with secondary focus.

    UI Expand
    titleFocusing with air objectives
    1. In NIS-Elements, click 10x, 20x or 40x to select the desired objective
      Info

      The 40x objective is the best Air objective because it has the greatest number of optical corrections (Plan Apochromat) and the largest numerical aperture (0.95).


    2. In NIS-Elements, select the optical configuration BF vis or the desired fluorescence (DAPI vis, ...) to activate the configuration
    3. Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp 
    4. Select the optical configuration Off to turn off the illumination
    5. Your sample is ready for acquisition!


    UI Expand
    titleFocusing with oil objectives

    After performing the first focus, in NIS-Elements:

    1. In NIS, click Escape, to lower the objectives

    2. Click 60x Oil or 100x Oil to select the desired objective. 

      Info

      The 100x objective has the greatest resolution (NA 1.45 vs 1.4)

    3. Place a single drop of oil on the objective
    4. In NIS-Elements, click again Escape to return the objective to its original position
    5. Select the optical configuration BF vis or the desired fluorescence (DAPI vis, ...) to activate the configuration
    6. Adjust the focus with the precision dial while looking through the eyepieces until the image is perfectly sharp 
    7. Select the optical configuration Off to turn off the illumination
    8. Your sample is ready for acquisition!
    Note
    titleImportant Reminders
  • Collect your samples, especially those in the microscope
    • Leave the microscope and workspace clean
    UI Expand
    titleStorage management
    • Files can be saved temporarily (during acquisition) to local C: drive (desktop)
    • At the end of each session, copy your data to your external drive and delete it from local C: drive
    • You can store your files on the D: drive (Data Storage). If you do, please create one folder per laboratory using the principal investigator's last name. Inside, create a folder per user using
    the following nomenclature (First Name_Last Name).
    Note
    titleImportant

    In any case, do not store your files on the C: drive.

    • the following nomenclature (First Name_Last Name).
    Note
    titleImportant

    In any case, do not store your files on the C: drive.

    UI Expand
    titleShutdown
    1. Save your data
    2. If the oil objective was used, clean it with lens cleaner and lens paper (not Kimwipes)
    3. Select the lowest magnification objective and press Escape to place the objectives in a safe position
    4. Close NIS-Elements
    5. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
    6. Turn off the computer
    7. Turn off the X-Cite exacte light (#2)
    8. Turn off the microscope power bar (#3)
    9. Cover the microscope
    Note
    titleImportant Reminders
    • Collect your samples, especially those in the microscope
    • Leave the microscope and workspace clean
    UI Expand
    titleFirst use
    AnchorFirstUseFirstUseWhen using the microscope for the first time, you need to import the microscope settings into the software. You will usually do this during the training session.
    This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.
    Note

    This process will delete all experiment protocols and reset the software to the original settings for this specific microscope.

  • If open, close the software NIS and wait until it is completely closed (up to 30 seconds)
  • On your Desktop open the Softwares folder
  • Open NIS Settings Utility
  • Click on the Import tab
  • Click on Browse
  • Navigate to your Documents
  • Select the file NIS Settings for Nikon TE2000E.bin
  • Click Select
  • Select all items
  • Click Import
  • Click OK
  • Close NIS Settings Utility
    • You can now reopen NIS
    Tabs Page
    idManuals
    titleManuals

    Available manuals

    Tabs Page
    idLog
    titleLog
    UI Expand
    expandedtrue
    titleTo do
    • Perfect Focus avec LED de 780 nm et avec filtre dans le trajet lumineux LP 750 nm (inadéquat pour le Cy5.5)

    • En lumière transmise, pour que le PFS fonctionne, il faut insérer 2 filtres IR dans le trajet lumineux: celui du PFS et celui du statif près de la lampe halogène

    UI Expand
    title2025-07-02 Insallation Bombardier 3132
    • Complete installation
    • Complete cleaning
    Tabs Page
    idTechnical Datasheet
    titleTechnical Datasheet

    Stand

    • Nikon TE2000-E inverted
    • Nikon T-HUBC Serial 750110
    • Nikon PFS T-PFS 810060
    • Nikon T-RCP Serial 750104

    Light sources

    • Transmitted Halogen light Nikon TE2-PS100W Serial 505320 12V
      • GIF Filter
      • Diffusion
      • Infra-red
      • Neutral color
      • Transmitted light shutter uniblitz VCM D1 Serial A607295
    • X-Cite exacte Model XCT10A 200 W Seria;l XCT10A-0337
      • EXFO Collimator 810-00030
      • Prior Shutter HF201HT Serial 7332
      • Prior 8 position motorized excitation filter wheel 61817
      • Nikon Manual Neutral density filters ND8 ND4
      • Manual Fluorescence field diaphragm
      • Manual Fluorescence shutter

    Condenser

    • Motorized condenser

    • Lens LWD NA 0.52

    • Filter turret 5 motorized positions

      1. A BF

    PhL
      1. DIC M
      2. Ph1
      3. Ph2
      4. Ph3

    Objectives

    • See description

    Stage

    • Prior Proscan 2 H30V4 Serial 62040
    • Remote control joystick Prior CS152v2 Serial R0772128
    •  Inserts
      • 1 slide
      • Multi-well plate
      • Adapter for 3cm dish

    Filters

    • See description

    Detector

    • Hamamtsu ORCA Flash V2 CMOS Model C11440-22CU Serial 002162 V2
    • Monochrome Camera 2048 x 2048 pixels
    • Pixel  6.5 um x 6.5 um
    • Force air cooled at -10C
    • Effective sensor area 13.312 mm (H) × 13.312 mm (V
    • Dark
    curent
    • current 0.06 electron/pixel/s
    • Reading noise 1.6 electrons (r.m.s) at standard scan
    • Dynamic range 1 : 18 000
    • Spectral response >40% between 400-850nm. Max 80% at 550nm

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    Workstation

    • HP Z800 Workstation
    • 2 x Intel Xeon X5650 @ 2.67GHz
    • RAM 24 GB DDR3 666 MHz  (6 x 4 GB)
    • OS 500 GB SSD 530 MB/s
    • 3 TB HD Data Storage (2 TB + 1 TB spanned volume) 130 MB/s
    • Video Card AMD FirePro V5800 1 GB dedicated memory
    • Monitor Dell 2407EFP-HC 1900x1200 60Hz
    • Software NIS-Elements AR v4.20.02 Build 988 HASP ID 4683DFE6
    • Core NIS-AR 2.3x STANDARD SUA Activation date 2010-07-07 SUA paid 2x SUA Expiration date 2013-08-07
    • Modules SRM ,Filter Wheel, ND (6 dimensions), Shutter, Stage XY axis

    Consumables

    • Liquid Light Guide
    excite
    • Excite exacte bulb
    • 12V halogen bulb


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    titleTroubleshooting & FAQ


    Troubleshooting

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    titleCamera Driver Failed

    This can happen when the acquisition software is turned on before the camera has fully initialized.

    • Turn off NIS-Elements
    Ensure the Initialize
    • Turn off the camera
    • Wait few seconds
    • Turn the camera back on
    • Wait until the camera has fully initialize (light at the back of the camera no longer blinks)
    • Start NIS-Elements

    FAQ

    UI Expand
    titleCan I use this microscope to look at cell in a dish?
    • Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
    • The objectives are optimized to image through thin glass bottom multi-well plates
    • You may also image specimen mounted between a slide and a 0.17mm thick coverslip
    • For long timelapse, be aware of photo-toxicity
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    idDemo Image
    titleNikon Ti2-E
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    idDemo Image
    titleDemo Image
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