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HP Z840 Deconvolution and Image Analysis Workstation

Roger Gaudry R-619

  • Applications

    • 2D and 3D Deconvolution

    • Image processing

    • Image Analysis

    • Quantification

  • Processor

    • 2 x Intel Xeon E5-2623 v4 2.60 GHz 

  • RAM

    • 128 GB (8x16GB) ECC DDR4 2400 MHz

  • Drives

    • 512 GB NVMe at 2500 MB/s OS
    • 4 TB (2x2TB) at 170 MB/s spanned volume for storage

  • Video Card

    • NVidia GTX 1080 with 8GB DDR5 dedicated memory

  • Monitor

    • HP Z24n 24" IPS display native resolution 1920 x 1200

  • OS
    • Windows 10 21H1
  • Softwares

    • ImageJ

    • FIJI

    • Microsoft Office 2016

    • NIS-Elements AR v5.02 64-bits

    • NIS Elements Offline Deconvolution v4.11 64-bits

  1. Turn on the computer
  2. Log in Windows using your UdM credentials
  3. Start-up NIS-Elements Offline Deconvolution
  1. Save your data
  2. Close NIS-Elements
  3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from the local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

In any case, your files should be removed from the C: drive.

  • Bring a network cable to connect directly to Ti2 microscope workstation
  • Windows 10 UdeM Image installation
  • Change the 2nd network adapter configuration with fix IP to enable for computer to computer communication
  • Change sharing policies of public document folder

Stand

  • Nikon Eclipse E600 upright Serial 725540

Light sources

  • Transmitted light Halogen 12V 100W Serial 01875599
  • Reflected light
    • Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
    • Bulb housing model LH M100C-1 Serial 039326

Condenser

  • Condenser Universal C-CU Serial 081901
  • Lens Dry NA 0.9
  • Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty

Objectives

  • 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
  • 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
  • 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
  • Empty
  • Empty
  • Empty
PositionNameBrandIDMagnificationNumerical ApertureImmersionTypeWorking distance (mm)Transmittance
(% [nm])		TechniqueCover glass thickness (mm)
110x/0.3 AirNikon10x/0.3 Air Plan Fluor Ph1 DLL10x0.3AirPlan Fluor16>75% [400-800]BF, Pol, PhC, Fluo0.17
2

40x/0.75 Air

Nikon40x/0.75 Air Plan Fluor Ph2 DLL40x

0.75

Air

Plan Fluor0.72>80% [400-750]BF, Pol, PhC, Fluo0.17
3

100x/1.3 Oil

Nikon100x/1.3 Oil Plan Fluor Ph3 DLL

100x

1.3
Oil
Plan Fluor0.2>75% [400-800]BF, Pol, PhC, Fluo0.17
4Empty









5Empty









6Empty









Filters

  • DAPI/Hoechst/AMCA

  • FITC/EGFP

  • TRITC/Rhodamine

  • TexasRed

PositionCube nameBrandIDExcitation FilterDichroic mirrorEmission Filter
1DAPI/Hoechst/AMCAChromaCube set 31000v2350/50x [325-375]400LP460/50m [435-485]
2FITC/EGFPChromaCube set 41001

480/40x [420-500]

505LP

535/50m [510-560]
3TRITC/RhodamineChroma

Cube set 41002c

545/30x [530-560]570LP620/60m [590-650]
4Texas RedChroma

Cube set 41004

560/55x [532-588]

595LP645/75m [607-682]
5Empty




6Empty




Detector

  • Color Camera Nikon DS-Ri2 Serial 701234

Workstation

  • HP Z440 Workstation
  • Intel Xeon E5-1630 v3 @ 3.7GHz
  • RAM 32 GB DDR4 2133 MHz  (4 x 8 GB)
  • OS 256GB SSD 550 MBs
  • 2TB HD Data Storage 150 MBs
  • Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
  • Monitor HP Z24i display 24' 1920x1200

Consumables

NIS-Elements shows an error: Camera Driver...

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

  • Turn off NIS-Elements
  • Turn on the camera (the microscope power bar and the camera switch on the top of the camera)
  • Check the USB conexion between the camera and the computer
  • Turn on NIS-Elements

Can I use this microscope to look at cell in a dish?

  • No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.

Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)

Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)


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