HP Z840 Deconvolution Workstation

Roger Gaudry R-619

Applications
- 2D and 3D Deconvolution
- Image processing
- Image analysis
- Quantification
Processor
- 2 x Intel Xeon E5-2623 v4 2.60 GHz
RAM
- 128 GB (8x16GB) ECC DDR4 2400 MHz
Drives
- OS 512GB NVMe at 2500 MB/s
- Data Storage 4TB at 170 MB/s (2x2TB spanned volumes)
Video Card
- nVidia GTX 1080 with 8GB DDR5 dedicated memory
Monitor
- HP Z24n 24" IPS display native resolution 1920 x 1200
OS
- Windows 10 21H1
Softwares
- ImageJ
- FIJI
- Microsoft Office 2016
- NIS-Elements AR v5.02 64-bits
- NIS-Elements Offline Deconvolution v4.11 64-bits

Save your data
Close NIS-Elements
Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive

Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
At the end of each session, copy your data to your external drive and delete it from the local C: drive
You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

In any case, your files should be removed from the C: drive.

Change GPO
Computer Configuration → Administrative Templates → All Settings
- Do not display the lock screen Enabled
- Set an active Power plan Enabled High Performance
- Select the Power button action (plugged in) Enabled Take no action
- Specify a custom active powe plan Enabled
- Specify the unattended sleep time out (plugged in) Enabled 0
- Turn off the display (plugged in) Enabled 0
- Turn off the hard disk (plugged in) Enabled 0
User Configuration → Administrative Templates → All Settings
- Enable screen saver Disabled
- Desktop Wallpaper Enabled C:\Admin Files\Source Files\DesktopBG.jpg Fit
- Screen saver timeout Enabled 0
- Password protect the screen saver Disabled

Computer Configuration -> Administrative Templates -> Control Panel -> Personalization

Windows 10 UdeM Image installation
Change the 2nd network adapter configuration with fix IP to enable for computer to computer communication
Change sharing policies of public document folder
Change GPO to disable screen saver

HP Z840 Workstation
2 x Intel Xeon E5-2623 v4 2.60 GHz
128 GB (8x16GB) ECC DDR4 2400 MHz
Drives
- OS 512GB NVMe at 2500 MB/s
- Data Storage 4TB at 170 MB/s (2x2TB spanned volumes)
Video Card nVidia GTX 1080 with 8GB DDR5 dedicated memory
Monitor HP Z24n 24" IPS display native resolution 1920 x 1200
OS Windows 10 21H1

Deconvolution processing not giving expected results...

This usually happens when the image files do not contain the correct metadata.

Make sure to save the files with all available metadata on the microscope used for the acquisition.
This usually requires to save the files in the software proprietary format (.nd for NIS-Elements and Metamorph, .czi for Zen,...)
You can always add the correct calibration values after acquisition (pixel size in XY and Z, wavelength, Z-step)

Can I use this workstation remotely?

Yes and No. You can't start the software remotely but you can start the software locally and then connect remotely to make sure all is running smoothly. To do so you need to request permission to the platform manager.

Which deconvolution method should I use?

There is no single answer
If you have a single plan you should choose 2D-deconvolution
If you have a Z-stack you should pick 3D-deconvolution.
If you want to have a quick view of the result you should use Fast Deconvolution.
Quality wise, the best results are acheived using blind and Landweber. However these methods required a long processing time (several hours for big files)
For more information about deconvolution method refer to paragraph 1.2.2 Choosing the deconvolution Method of the NIS_Offline Deconvolution_Manual.pdf

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